Multifocal animated imaging of changes in cellular oxygen and calcium concentrations and membrane potential within the intact adult mouse carotidbody ex vivo

American Journal of Physiology - Cell Physiology

Volumn 301, Issue 2, 2011, Pages

  Wotzlaw, Christoph ^a   Bernardini, André ^a   Berchner Pfannschmidt, Utta ^a   Papkovsky, Dmitri ^b   Acker, Helmut ^a   Fandrey, Joachim ^a  

^a UNIVERSITY OF DUISBURG ESSEN   (Germany)

^b UNIVERSITY COLLEGE CORK   (Ireland)

Author keywords

Carotid body type I cells; Oxygen sensing

Indexed keywords

CALCIUM; OXYGEN; TYROSINE 3 MONOOXYGENASE;

ANIMAL TISSUE; ARTICLE; CALCIUM CELL LEVEL; CAROTID BODY; CELL HYPOXIA; CELL MEMBRANE DEPOLARIZATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; EX VIVO STUDY; IMMUNOFLUORESCENCE; IMMUNOHISTOCHEMISTRY; MALE; MEMBRANE POTENTIAL; MOUSE; NONHUMAN; OSCILLATION; OXYGEN SENSING; PRIORITY JOURNAL; TISSUE OXYGENATION;

ANILINE COMPOUNDS; ANIMALS; CALCIUM; CALCIUM SIGNALING; CAROTID BODY; CELL HYPOXIA; FEMALE; FLUORESCEINS; FLUORESCENT ANTIBODY TECHNIQUE; FLUORESCENT DYES; IMMUNOHISTOCHEMISTRY; KINETICS; MEMBRANE POTENTIALS; MICE; MICE, INBRED C57BL; MICROSCOPY, FLUORESCENCE; OSCILLOMETRY; OXYGEN; TYROSINE 3-MONOOXYGENASE; VOLTAGE-SENSITIVE DYE IMAGING;

EID: 79961083401     PISSN: 03636143     EISSN: 15221563     Source Type: Journal    
DOI: 10.1152/ajpcell.00508.2010     Document Type: Article

References (30)

1. Bright GR, Agani FH, Haque U, Overholt JL, Prabhakar NR. Heterogeneityin cytosolic calcium responses to hypoxia in carotid body cells. Brain Res 706: 297-302, 1996.
2. Buckler KJ. TASK-like potassium channels and oxygen sensing in thecarotid body. Respir Physiol Neurobiol 157: 55-64, 2007.
3. Buckler KJ, Williams BA, Honore E. An oxygen-, acid- and anaestheticsensitiveTASK-like background potassium channel in rat arterial chemoreceptorcells. J Physiol 525: 135-142, 2000.
4. Carroll JL, Agarwal A. Development of ventilatory control in infants. Paediatr Respir Rev 11: 199-207, 2010.
5. Conchello JA, Lichtman JW. Optical sectioning microscopy. Nat Methods2: 920-931, 2005.
6. Dmitriev RI, Zhdanov AV, Ponomarev GV, Yashunski DV, Papkovsky DB. Intracellular oxygen-sensitive phosphorescent probes based oncell-penetrating peptides. Anal Biochem 398: 24-33, 2010.
7. Donnelly DF. Orthodromic spike generation from electrical stimuli in therat carotid body: implications for the afferent spike generation process. JPhysiol 580: 275-284, 2007.
8. Donnelly DF, Kholwadwala D. Hypoxia decreases intracellular calciumin adult rat carotid body glomus cells. J Neurophysiol 67: 1543-1551,1992.
9. He L, Liu X, Chen J, Dinger B, Stensaas L, Fidone S. Modulation ofchronic hypoxia-induced chemoreceptor hypersensitivity by NADPH oxidasesubunits in rat carotid body. J Appl Physiol 108: 1304-1310, 2010.
10. +-channelin the mouse carotid body. Respir Physiol Neurobiol 172: 122-128, 2010.
11. Kameda Y, Nishimaki T, Takeichi M, Chisaka O. Homeobox genehoxa3 is essential for the formation of the carotid body in the mouseembryos. Dev Biol 247: 197-209, 2002.
12. Kemp PJ, Telezhkin V, Wilkinson WJ, Mears R, Hanmer SB, Gadeberg HC, Muller CT, Riccardi D, Brazier SP. Enzyme-linked oxygensensing by potassium channels. Ann NY Acad Sci 1177: 112-118, 2009.
13. + channel in ratcarotid body glomus cells. J Physiol 587: 2963-2975, 2009.
14. Lahiri S, Ehleben W, Acker H. Chemoreceptor discharges and cytochromeredox changes of the rat carotid body: role of heme ligands. ProcNatl Acad Sci USA 96: 9427-9432, 1999.
15. Li Q, Sun B, Wang X, Jin Z, Zhou Y, Dong L, Jiang LH, Rong W. Acrucial role for hydrogen sulfide in oxygen sensing via modulating largeconductance calcium-activated potassium channels. Antioxid Redox Signal12: 1179-1189, 2010.
16. Ortega-Saenz P, Levitsky KL, Marcos-Almaraz MT, Bonilla-Henao V, Pascual A, Lopez-Barneo J. Carotid body chemosensory responses inmice deficient of TASK channels. J Gen Physiol 135: 379-392, 2010.
17. Pang L, Eyzaguirre C. Different effects of hypoxia on the membranepotential and input resistance of isolated and clustered carotid bodyglomus cells. Brain Res 575: 167-173, 1992.
18. Pang L, Eyzaguirre C. Hypoxia affects differently the intracellular pH ofclustered and isolated glomus cells of the rat carotid body. Brain Res 623:349-355, 1993.
19. Perez-Garcia MT, Colinas O, Miguel-Velado E, Moreno-Dominguez A, Lopez-Lopez JR. Characterization of the Kv channels of mousecarotid body chemoreceptor cells and their role in oxygen sensing. JPhysiol 557: 457-471, 2004.
20. Perocchi F, Gohil VM, Girgis HS, Bao XR, McCombs JE, Palmer AE, Mootha VK. MICU1 encodes a mitochondrial EF hand protein requiredfor Ca(2+) uptake. Nature 467: 291-296, 2010.
21. Pierson R, Johnson H, Harris G, Keefe H, Paulsen JS, Andreasen NC, Magnotta VA. Fully automated analysis using BRAINS: AutoWorkup. Neuroimage 54: 328-336, 2011.
22. Ross FA, Rafferty JN, Dallas ML, Ogunbayo O, Ikematsu N, Mc-Clafferty H, Tian L, Widmer H, Rowe IC, Wyatt CN, Shipston MJ, Peers C, Hardie DG, Evans AM. Selective expression in carotid bodytype I cells of a single splice variant of the large conductance calcium- andvoltage-activated potassium channel confers regulation by AMP-activatedprotein kinase. J Biol Chem 286: 11929-11936, 2011.
23. Sakadzic S, Roussakis E, Yaseen MA, Mandeville ET, Srinivasan VJ, Arai K, Ruvinskaya S, Devor A, Lo EH, Vinogradov SA, Boas DA. Two-photon high-resolution measurement of partial pressure of oxygen incerebral vasculature and tissue. Nat Methods 7: 755-759, 2010.
24. Schneggenburger R, Neher E. Presynaptic calcium and control of vesiclefusion. Curr Opin Neurobiol 15: 266-274, 2005.
25. Streller T, Huckstorf C, Pfeiffer C, Acker H. Unusual cytochromea(592) with low PO2 affinity correlates as putative oxygen sensor with ratcarotid body chemoreceptor discharge. FASEB J 16: 1277-1279, 2002.
26. Weir EK, Lopez-Barneo J, Buckler KJ, Archer SL. Acute oxygensensingmechanisms. N Engl J Med 353: 2042-2055, 2005.
27. Wotzlaw C, Berchner-Pfannschmidt U, Fandrey J, Acker H. Twophotonimaging of cellular activities in oxygen sensing tissues. MicroscMicronanal 14: 519-525, 2008.
28. Wotzlaw C, Otto T, Berchner-Pfannschmidt U, Metzen E, Acker H, Fandrey J. Optical analysis of the HIF-1 complex in living cells by FRETand FRAP. FASEB J 21: 700-707, 2007.
29. Yi L, Morgan JT, Ragsdale SW. Identification of a thiol/disulfide redoxswitch in the human BK channel that controls its affinity for heme and CO. J Biol Chem 285: 20117-20127, 2010.
30. Zhang M, Buttigieg J, Nurse CA. Neurotransmitter mechanisms mediatinglow-glucose signalling in cocultures and fresh tissue slices of ratcarotid body. J Physiol 578: 735-750, 2007.