Development of a 16S rRNA gene primer and PCR-Restriction fragment length Polymorphism method for rapid detection of members of the genus megasphaera and species-level identification

Applied and Environmental Microbiology

Volumn 77, Issue 15, 2011, Pages 5533-5535

  Ohnishi, Akihiro ^a   Abe, Shinko ^a   Nashirozawa, Shiho ^a   Shimada, Sayaka ^a   Fujimoto, Naoshi ^a   Suzuki, Masaharu ^a  

^a TOKYO UNIVERSITY OF AGRICULTURE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

16S RRNA GENE; DIRECT DETECTION; ENVIRONMENTAL SAMPLE; FRAGMENT LENGTHS; HUMAN HEALTH; RAPID DETECTION; RENEWABLE ENERGIES;

GENES;

RNA;

BACTERIAL DNA; PRIMER DNA; RNA 16S;

BACTERIUM; DETECTION METHOD; ENERGY EFFICIENCY; FOOD; FRAGMENTATION; GENETIC ANALYSIS; IDENTIFICATION METHOD; POLYMERASE CHAIN REACTION; POLYMORPHISM; RENEWABLE RESOURCE;

ARTICLE; GENETICS; ISOLATION AND PURIFICATION; MEGASPHAERA; METHODOLOGY; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SEQUENCE ALIGNMENT;

DNA PRIMERS; DNA, BACTERIAL; MEGASPHAERA; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; RNA, RIBOSOMAL, 16S; SEQUENCE ALIGNMENT;

MEGASPHAERA;

EID: 79961037694     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00359-11     Document Type: Article

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