Inhibition of Human Papillomavirus Expression Using DNAzymes

Methods in Molecular Biology

Volumn 764, Issue , 2011, Pages 317-335

  Benítez Hess, María Luisa ^a   Reyes Gutiérrez, Pablo ^a   Alvarez Salas, Luis Marat ^a  

^a DEPARTAMENTO DE FÍSICA   (Mexico)

Author keywords

10 23 DNAzyme; antisense; antisense DNA; catalytic DNA; DNA; DNA structure; DNAzyme; DNAzyme expression; papillomavirus

Indexed keywords

ANTISENSE OLIGONUCLEOTIDE; DEOXYRIBOZYME; MESSENGER RNA; OLIGODEOXYRIBONUCLEOTIDE; SINGLE STRANDED DNA; VIRUS RNA;

APOPTOSIS; ARTICLE; CELL SURVIVAL; CHEMISTRY; DRUG EFFECT; ENZYME ACTIVE SITE; FEMALE; GENETIC TRANSFECTION; GROWTH, DEVELOPMENT AND AGING; HUMAN; HUMAN PAPILLOMAVIRUS TYPE 16; METABOLISM; METHODOLOGY; MOLECULARLY TARGETED THERAPY; PAPILLOMAVIRUS INFECTION; POLYACRYLAMIDE GEL ELECTROPHORESIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; THIN LAYER CHROMATOGRAPHY; TUMOR CELL LINE; UTERINE CERVIX TUMOR; VIROLOGY; VIRUS REPLICATION;

APOPTOSIS; CATALYTIC DOMAIN; CELL LINE, TUMOR; CELL SURVIVAL; CHROMATOGRAPHY, THIN LAYER; DNA, CATALYTIC; DNA, SINGLE-STRANDED; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FEMALE; HUMAN PAPILLOMAVIRUS 16; HUMANS; MOLECULAR TARGETED THERAPY; OLIGODEOXYRIBONUCLEOTIDES; OLIGONUCLEOTIDES, ANTISENSE; PAPILLOMAVIRUS INFECTIONS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; RNA, VIRAL; TRANSFECTION; UTERINE CERVICAL NEOPLASMS; VIRUS REPLICATION;

HUMAN PAPILLOMAVIRUS; PAPILLOMAVIRIDAE;

EID: 79960969165     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-188-8_21     Document Type: Chapter

References (35)

1. Baum, D. A., and Silverman, S. K. (2008) Deoxyribozymes: useful DNA catalysts in vitro and in vivo. Cell Mol. Life Sci. 65, 2156–2174.
2. Santoro, S. W., and Joyce, G. F. (1997) A general purpose RNA-cleaving DNA enzyme. Proc. Natl. Acad. Sci. USA 94, 4262–4266.
3. Santoro, S. W., and Joyce, G. F. (1998) Mechanism and utility of an RNA-cleaving DNA enzyme. Biochemistry 37, 13330–13342.
4. Bhindi, R., Fahmy, R. G., Lowe, H. C., Chesterman, C. N., Dass, C. R., Cairns, M. J., Saravolac, E. G., Sun, L. Q., and Khachigian, L. M. (2007) Brothers in arms. DNA enzymes, short interfering RNA, and the emerging wave of small-molecule nucleic acid-based gene-silencing strategies. Am. J. Pathol. 171, 1079–1088.
5. Dass, C. R., Choong, P. F., and Khachigian, L. M. (2008) DNAzyme technology and cancer therapy: cleave and let die. Mol. Cancer Ther. 7, 243–251.
6. Alvarez-Salas, L. M., and DiPaolo, J. A. (2007) Molecular approaches to cervical cancer therapy. Curr. Drug Discov. Technol. 4, 208–219.
7. Faulhammer, D., and Famulok, M. (1997) Characterization and divalent metal-ion dependence of in vitro selected deoxyribozymes which cleave DNA/RNA chimeric oligonucleotides. J. Mol. Biol. 269, 188–202.
8. Cairns, M. J., King, A., and Sun, L. Q. (2000) Nucleic acid mutation analysis using catalytic DNA. Nucleic Acids Res. 28, E9.
9. Yuan, B. F., Xue, Y., Luo, M., Hao, Y. H., and Tan, Z. (2007) Two DNAzymes targeting the telomerase mRNA with large difference in Mg2+ concentration for maximal cat- alytic activity. Int. J. Biochem. Cell Biol. 39, 1119–1129.
10. Takamori, K., Kubo, T., Zhelev, Z., Rumiana, B., Ohba, H., Doi, K., and Fujii, M. (2005) Suppression of bcr/abl chimeric gene by conjugate DNA enzymes in human cells. Nucl. Acids Symp. Ser. (Oxf.) 49, 333–334.
11. Reyes-Gutierrez, P., and Alvarez-Salas, L. M. (2009) Cleavage of HPV-16 E6/E7 mRNA mediated by modified 10-23 deoxyribozymes. Oligonucleotides 19, 233–242.
12. Pirisi, L., Yasumoto, S., Feller, M., Doniger, J., and DiPaolo, J. A. (1987) Transformation of human fibroblasts and keratinocytes with human papillomavirus type 16 DNA. J. Virol. 61, 1061–1066.
13. Durst, M., Dzarlieva-Petrusevska, R. T., Boukamp, P., Fusenig, N. E., and Gissmann, L. (1987) Molecular and cytogenetic analysis of immortalized human primary keratinocytes obtained after transfection with human papillomavirus type 16 DNA. Oncogene 1, 251–256.
14. Alvarez-Salas, L. M., Benitez-Hess, M. L., and DiPaolo, J. A. (2003) Advances in the development of ribozymes and antisense oligodeoxynucleotides as antiviral agents for human papillomaviruses. Antivir. Ther. 8, 265–278.
15. Broker, T. R. (1987) Structure and genetic expression of papillomaviruses. Obstet. Gynecol. Clin. North Am. 14, 329–348.
16. Smotkin, D., Prokoph, H., and Wettstein, F. O. (1989) Oncogenic and nononcogenic human genital papillomaviruses generate the E7 mRNA by different mechanisms. J. Virol. 63, 1441–1447.
17. Schneider-Gadicke, A., and Schwarz, E. (1986) Different human cervical carcinoma cell lines show similar transcription patterns of human papillomavirus type 18 early genes. EMBO J. 5, 2285–2292.
18. DiPaolo, J. A., Popescu, N. C., Alvarez, L., and Woodworth, C. D. (1993) Cellular and molecular alterations in human epithelial cells transformed by recombinant human papillomavirus DNA. Crit. Rev. Oncog. 4, 337–360.
19. Duensing, S., and Munger, K. (2002) The human papillomavirus type 16 E6 and E7 oncoproteins independently induce numerical and structural chromosome instability. Cancer Res. 62, 7075–7082.
20. Alvarez-Salas, L. M., Cullinan, A. E., Siwkowski, A., Hampel, A., and DiPaolo, J. A. (1998) Inhibition of HPV-16 E6/E7 immortalization of normal keratinocytes by hairpin ribozymes. Proc. Natl. Acad. Sci. USA 95, 1189–1194.
21. Alvarez-Salas, L. M., Arpawong, T. E., and DiPaolo, J. A. (1999) Growth inhibition of cervical tumor cells by antisense oligodeoxynucleotides directed to the human papillomavirus type 16 E6 gene. Antisense Nucl. Acid Drug Dev. 9, 441–450.
22. Butz, K., Denk, C., Ullmann, A., Scheffner, M., and Hoppe-Seyler, F. (2000) Induction of apoptosis in human papillomaviruspositive cancer cells by peptide aptamers targeting the viral E6 oncoprotein. Proc. Natl. Acad. Sci. USA 97, 6693–6697.
23. Jiang, M., and Milner, J. (2002) Selective silencing of viral gene expression in HPV-positive human cervical carcinoma cells treated with siRNA, a primer of RNA interference. Oncogene 21, 6041–6048.
24. Romani, A. M., and Scarpa, A. (2000) Regulation of cellular magnesium. Front Biosci. 5, D720–D734.
25. Alvarez-Salas, L. M. (2008) Nucleic acids as therapeutic agents. Curr. Top Med. Chem. 8, 1379–1404.
26. Santoro, S. W., Joyce, G. F., Sakthivel, K., Gramatikova, S., and Barbas, C. F. (2000) RNA cleavage by a DNA enzyme with extended chemical functionality. J. Am. Chem. Soc. 122, 2433–2439.
27. Cieslak, M., Niewiarowska, J., Nawrot, M., Koziolkiewicz, M., Stec, W. J., and Cierniewski, C. S. (2002) DNAzymes to beta 1 and beta 3 mRNA down-regulate expression of the targeted integrins and inhibit endothelial cell capillary tube formation in fibrin and matrigel. J. Biol. Chem. 277, 6779–6787.
28. ′-O-methylribonucleotide)-effectors. Nucl. Acids Symp. Ser. (Oxf.) 52, 525–526.
29. Vester, B., Hansen, L. H., Lundberg, L. B., Babu, B. R., Sorensen, M. D., Wengel, J., and Douthwaite, S. (2006) Locked nucleoside analogues expand the potential of DNAzymes to cleave structured RNA targets. BMC Mol. Biol. 7, 19.
30. Guapillo, M. R., Marquez-Gutiérrez, M. A., Benitez-Hess, M. L., and Alvarez-Salas, L. M. (2006) A bacterial reporter system for the evaluation of antisense oligodeoxynucleotides directed against human papillomavirus type 16 (HPV-16). Arch. Med. Res. 37, 584–592.
31. Marquez-Gutierrez, M. A., Benitez-Hess, M. L., DiPaolo, J. A., and Alvarez-Salas, L. M. (2007) Effect of combined antisense oligodeoxynucleotides directed against the human papillomavirus type 16 on cervical carcinoma cells. Arch. Med. Res. 38, 730–738.
32. Aquino-Jarquin, G., Benitez-Hess, M. L., DiPaolo, J. A., and Alvarez-Salas, L. M. (2008) A triplex ribozyme expression system based on a single hairpin ribozyme. Oligonucleotides 18, 213–224.
33. DeYoung, M. B., Siwkowski, A., and Hampel, A. (1997) Determination of catalytic parameters for hairpin ribozymes. Methods Mol. Biol. 74, 209–220.
34. Baker, C. C., Phelps, W. C., Lindgren, V., Braun, M. J., Gonda, M. A., and Howley, P. M. (1987) Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines. J. Virol. 61, 962–971.
35. Yee, C., Krishnan-Hewlett, I., Baker, C. C., Schlegel, R., and Howley, P. M. (1985) Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119, 361–366.