Molecular cloning and analysis of the UDP-Glucose Pyrophosphorylase in Streptococcus equi subsp. zooepidemicus

Molecular Biology Reports

Volumn 38, Issue 4, 2011, Pages 2751-2760

  Ma, Zhe ^a   Fan, Hong Jie ^a   Lu, Cheng Ping ^a  

^a NANJING AGRICULTURAL UNIVERSITY   (China)

Author keywords

Function; S. equi subsp. zooepidemicus; Structure; UDP Glucose Pyrophosphorylase

Indexed keywords

ENZYME ANTIBODY; HYALURONIC ACID; UNCLASSIFIED DRUG; URIDINE TRIPHOSPHATE GLUCOSE 1 PHOSPHATE URIDYLYLTRANSFERASE; URIDINE TRIPHOSPHATE GLUCOSE 1 PHOSPHATE URIDYLYLTRANSFERASE ANTIBODY; OLIGONUCLEOTIDE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL METABOLISM; BACTERIAL VIRULENCE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME PURIFICATION; GENE AMPLIFICATION; GENE LOCATION; GENE OVEREXPRESSION; GENOME SIZE; GROWTH CURVE; MOLECULAR CLONING; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY; STREPTOCOCCUS EQUI ZOOEPIDEMICUS; TEMPERATURE; UGPASE GENE; AMINO ACID SEQUENCE; BIOSYNTHESIS; CLUSTER ANALYSIS; DNA SEQUENCE; ENZYMOLOGY; GENETICS; IMMUNOBLOTTING; KINETICS; METABOLISM; MOLECULAR GENETICS; PATHOGENICITY; PH; PHYLOGENY; STREPTOCOCCUS EQUI; VIRULENCE;

BACTERIA (MICROORGANISMS); STREPTOCOCCUS EQUI SUBSP. ZOOEPIDEMICUS;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; CLUSTER ANALYSIS; HYALURONIC ACID; HYDROGEN-ION CONCENTRATION; IMMUNOBLOTTING; KINETICS; MOLECULAR SEQUENCE DATA; OLIGONUCLEOTIDES; PHYLOGENY; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; STREPTOCOCCUS EQUI; TEMPERATURE; UTP-GLUCOSE-1-PHOSPHATE URIDYLYLTRANSFERASE; VIRULENCE;

EID: 79960968986     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-010-0420-8     Document Type: Article

References (26)

1. Weissbom AE, Liu Q, Rumley MK (1994) UTP: alpha-D-glucose-l-phosphate uridylytransferase of Escheichia coli: isolation and DNA sequence of the galU gene and purification of the enzyme. J Bacteriol 176:2611-2618
2. Kleczkowski LA, Martz F, Wilczynska M (2005) Factors affecting oligomerization status of udp-glucose pyrophosphorylase. Phytochemistry 66(24):2815-2821
3. Martz F, Wilczynska M, Kleczkowski LA (2002) Oligomerization status, with the monomer as active species, defines catalytic efficiency of UDP-glucose pyrophosphorylase. Biochem J 367(Pt 1):295-300
4. Gandhi TK, Zhong J, Mathivanan S, Karthick L, Chandrika KN, Mohan SS, Sharma S, Pinkert S, Nagaraju S, Periaswamy B, Mishra G, Nandakumar K, Shen B, Deshpande N, Nayak R, Sarker M, Boeke JD, Parmigiani G, Schultz J, Bader JS, Pandey A (2006) Analysis of the human protein interactome and comparison with yeast, worm and fly interaction datasets. Nat Genet 38(3):285-293
5. Crater DL, Dougherty BA, van de Rijn I (1995) Molecular characterization of hasC from an operon required for hyaluronic acid synthesis in group A streptococci. Demonstration of UDPglucose pyrophosphorylase activity. J Biol Chem 270(48): 28676-28680
6. Las Heras A, Vela AI, Fernandez E, Legaz E, Dominguez L, Fernandez-Garayzabal JF (2002) Unusual outbreak of clinical mastitis in dairy sheep caused by Streptococcus equi subsp. Zooepidemicus. J Clin Microbiol 40(3):1106-1108
7. Salasia SI, Wibawan IW, Pasaribu FH, Abdulmawjood A, Lammler C (2004) Persistent occurrence of a single Streptococcus equi subsp. Zooepidemicus clone in the pig and monkey population in indonesia. J Vet Sci 5(3):263-265
8. Ural O, Tuncer I, Dikici N, Aridogan B (2003) Streptococcus zooepidemicus meningitis and bacteraemia. Scand J Infect Dis 35(3):206-207
9. Mao Y, Fan H, Lu C (2008) Immunoproteomic assay of extracellular proteins in Streptococcus equi ssp. Zooepidemicus. FEMS Microbiol Lett 286(1):103-109
10. Lai X, Wu J, Chen S, Zhang X, Wang H (2008) Expression, purification, and characterization of a functionally active Mycobacterium tuberculosis UDP-glucose pyrophosphorylase. Protein Expr Purif 61(1):50-56
11. Arnold KBL, Kopp J, Schwede T (2006) The swiss-model workspace: a web-based environment for protein structure homology modelling. Bioinformatics 22:195-201
12. Kiefer FAK, Kunzli M, Bordoli L, Schwede T (2009) The swissmodel repository and associated resources. Nucleic Acids Res 37:D387-D392
13. Peitsch MC (1995) Protein modeling by e-mail. Bio/Technology 13:658-660
14. Lun S, Willson PJ (2004) Expression of green fluorescent protein and its application in pathogenesis studies of serotype 2 Streptococcus suis. J Microbiol Methods 56(3):401-412
15. Zhang J, Hao N, Chen GQ (2006) Effect of expressing polyhydroxybutyrate synthesis genes (phbcab) in Streptococcus zooepidemicus on production of lactic acid and hyaluronic acid. Appl Microbiol Biotechnol 71(2):222-227
16. Silveira EDA-FM, Guimaraes LA, da Silva FR, Carneiro VT (2009) Selection of reference genes for quantitative real-time PCR expression studies in the apomictic and sexual grass Brachiaria brizantha. BMC Plant Biol 9(1):84
17. Carey CMKM, Thompson S (2009) Escherichia coli o157:H7 stress and virulence gene expression on romaine lettuce using comparative real-time PCR. J Microbiol Methods 77(2):235-242
18. Gao JGA, Scott JR, Churchward G (2005) Binding of the global response regulator protein covR to the sag promoter of Streptococcus pyogenes reveals a new mode of covR-DNA interaction. J Biol Chem 280(47):38948-38956
19. Blank LMHP, Nielsen LK (2008) Evolution of the hyaluronic acid synthesis (has) operon in Streptococcus zooepidemicus and other pathogenic streptococci. J Mol Evol 67(1):13-22
20. Geisler M, Wilczynska M, Karpinski S, Kleczkowski LA (2004) Toward a blueprint for UDP-glucose pyrophosphorylase structure/ function properties: Homology-modeling analyses. Plant Mol Biol 56(5):783-794
21. Gottschalk LM, Bon EP, Nobrega R (2008) Lignin peroxidase from Streptomyces viridosporus T7A: enzyme concentration using ultrafiltration. Appl Biochem Biotechnol 147(1-3):23-32
22. Jensen PR, Hammer K (1998) The sequence of spacers between the consensus sequences modulates the strength of prokaryotic promoters. Appl Environ Microbiol 64(1):82-87
23. Chen WY, Marcellin E, Hung J, Nielsen LK (2009) Hyaluronan molecular weight is controlled by UDP-N-acetylglucosamine concentration in Streptococcus zooepidemicus. J Biol Chem 284(27):18007-18014
24. Chassaing DAF (2007) The lmo1078 gene encoding a putative UDP-glucose pyrophosphorylase is involved in growth of Listeria monocytogenes at low temperature. FEMS Microbiol Lett 275(1):31-37
25. Wibawan IW, Pasaribu FH, Utama IH, Abdulmawjood A, Lammler C (1999) The role of hyaluronic acid capsular material of Streptococcus equi subsp. Zooepidemicus in mediating adherence to Hela cells and in resisting phagocytosis. Res Vet Sci 67(2):131-135
26. Vilches S, Canals R, Wilhelms M, Salo MT, Knirel YA, Vinogradov E, Merino S, Tomas JM (2007) Mesophilic aeromonas UDP-glucose pyrophosphorylase (galU) mutants show two types of lipopolysaccharide structures and reduced virulence. Microbiology 153(Pt 8):2393-2404