Expression of green fluorescent protein and its application in pathogenesis studies of serotype 2 Streptococcus suis

Journal of Microbiological Methods

Volumn 56, Issue 3, 2004, Pages 401-412

  Lun, Shichun ^a   Willson, P J ^a  

^a UNIVERSITY OF SASKATCHEWAN   (Canada)

Author keywords

Flow cytometry; Gram positive bacteria; Non invasive visible marker; Pathogenesis; Phagocyte association

Indexed keywords

GREEN FLUORESCENT PROTEIN;

ALLELE; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BACTERIAL STRAIN; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; EPIFLUORESCENCE MICROSCOPY; FLUORESCENCE ACTIVATED CELL SORTING; GENETIC TRANSFORMATION; LIGHT; MICROBIOLOGICAL EXAMINATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PATHOGENESIS; PHAGOCYTE; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; SEROTYPE; SHUTTLE VECTOR; STREPTOCOCCUS INFECTION; STREPTOCOCCUS SUIS; SWINE;

POSIBACTERIA; STREPTOCOCCUS; STREPTOCOCCUS SUIS; SUS SCROFA; UNIDENTIFIED SHUTTLE VECTOR;

EID: 1142309598     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2003.11.012     Document Type: Article

References (30)

1. Acebo P., Nieto C., Corrales M., Espinosa M., Lopez P. Quantitative detection of Streptococcus pneumoniae cells harbouring single or multiple copies of the gene encoding the green fluorescent protein. Microbiology. 146:2000;1267-1273.
2. Aspiras M., Kazmerzak K., Kolenbrander P., McNab R., Hardegen N., Jenkinson H. Expression of green fluorescent protein in Streptococcus gordonii DL1 and its use as a species-specific marker in coadhesion with Streptococcus oralis 34 in saliva-conditioned biofilms in vitro. Appl. Environ. Microbiol. 66:2000;4074-4083.
3. Brazeau C., Gottschalk M., Vincelette S., Martineau-Doize B. In vitro phagocytosis and survival of Streptococcus suis capsular type 2 inside murine macrophages. Microbiology. 142:1996;1231-1237.
4. Burg N., Pillinger M. The neutrophil: function and regulation in innate and humoral immunity. Clin. Immunol. 99:2001;7-17.
5. Busque P., Higgins R., Senechal S., Marchand R., Quessy S. Simultaneous flow cytometric measurement of Streptococcus suis phagocytosis by polymorphonuclear and mononuclear blood leukocytes. Vet. Microbiol. 63:1998;229-238.
6. Celli J., Finlay B. Bacterial avoidance of phagocytosis. Trends Microbiol. 10:2002;232-237.
7. Chalfie M., Tu Y., Euskirchen G., Ward W., Prasher D. Green fluorescent protein as a marker for gene expression. Science. 263:1994;802-805.
8. Chanter N., Jones P., Alexander T. Meningitis in pigs caused by Streptococcus suis - a speculative review. Vet. Microbiol. 36:1993;39-55.
9. Cormack B., Valdivia R., Falkow S. FACS-optimized mutants of the green fluorescent protein (GFP). Gene. 173:1996;33-38.
10. du Manoir J., Albright B., Stevenson G., Thompson S., Mitchell G., Clark M., Caswell J. Variability of neutrophil and pulmonary alveolar macrophage function in swine. Vet. Immunol. Immunopathol. 89:2002;175-186.
11. Gomis S., Godson D., Beskorwayne T., Wobeser G., Potter A. Modulation of phagocytic function of bovine mononuclear phagocytes by Haemophilus somnus. Microb. Pathog. 22:1997;13-21.
12. Gottschalk M., Higgins R., Jacques M., Beaudoin M., Henrichsen J. Characterization of six new capsular types (23 through 28) of Streptococcus suis. J. Clin. Microbiol. 29:1991;2590-2594.
13. Gottschalk M., Higgins R., Jacques M., Dubreuil D. Production and characterization of two Streptococcus suis capsular type 2 mutants. Vet. Microbiol. 30:1992;59-71.
14. Gottschalk M., Lacouture S., Dubreuil J. Characterization of Streptococcus suis capsular type 2 haemolysin. Microbiology. 141:1995;189-195.
15. Hansen M., Palmer R.J., Udsen C., White D., Molin S. Assessment of GFP fluorescence in cells of Streptococcus gordonii under conditions of low pH and low oxygen concentration. Microbiology. 147:2001;1383-1391.
16. Heim R., Prasher D., Tsien R. Wavelength mutations and posttranslational autoxidation of green fluorescent protein. Proc. Natl. Acad. Sci. U. S. A. 91:1994;12501-12504.
17. Heinzelmann M., Gardner S., Mercer-Jones M., Roll A., Polk H.J. Quantification of phagocytosis in human neutrophils by flow cytometry. Microbiol. Immunol. 43:1999;505-512.
18. Higgins R., Gottschalk M., Boudreau M., Lebrun A., Henrichsen J. Description of six new capsular types (29-34) of Streptococcus suis. J. Vet. Diagn. Invest. 7:1995;405-406.
19. Lun S., Perez-Casal J., Connor W., Willson P. Role of suilysin in pathogenesis of Streptococcus suis capsular serotype 2. Microb. Pathog. 34:2003;27-37.
20. Rodriguez M., Van der Pol W., Van de Winkel J. Flow cytometry-based phagocytosis assay for sensitive detection of opsonic activity of pneumococcal capsular polysaccharide antibodies in human sera. J. Immunol. Methods. 252:2001;33-44.
21. Salasia S., Lammler C., Herrmann G. Properties of a Streptococcus suis isolate of serotype 2 and two capsular mutants. Vet. Microbiol. 45:1995;151-156.
22. Salles M., Perez-Casal J., Willson P., Middleton D. Changes in the leucocyte subpopulations of the palatine tonsillar crypt epithelium of pigs in response to Streptococcus suis type 2 infection. Vet. Immunol. Immunopathol. 87:2002;51-63.
23. Scott K., Mercer D., Glover L., Flint H. The green fluorescent protein as a visible marker for lactic acid bacteria in complex ecosystems. FEMS Microbiol. Ecol. 26:1998;219-230.
24. Somkuti G., Steinberg D. Promoter activity of the pER341-borne ST(Phsp) in heterologous gene expression in Escherichia coli and Streptococcus thermophilus(1). FEMS Microbiol. Lett. 179:1999;431-436.
25. Staats J., Feder I., Okwumabua O., Chengappa M. Streptococcus suis: past and present. Vet. Res. Commun. 21:1997;381-407.
26. Tsien R. The green fluorescent protein. Ann. Rev. Biochem. 67:1998;509-544.
27. Valdivia R., Hromockyj A., Monack D., Ramakrishnan L., Falkow S. Applications for green fluorescent protein (GFP) in the study of host-pathogen interactions. Gene. 173:1996;47-52.
28. White-Owen C., Alexander J., Sramkoski R., Babcock G. Rapid whole-blood microassay using flow cytometry for measuring neutrophil phagocytosis. J. Clin. Microbiol. 30:1992;2071-2076.
29. Williams A., Blakemore W. Monocyte-mediated entry of pathogens into the central nervous system. Neuropathol. Appl. Neurobiol. 16:1990;377-392.
30. Zimmer M. Green fluorescent protein (GFP): applications, structure, and related photophysical behavior. Chem. Rev. 102:2002;759-781.