Evaluation of oral antiseptic rinsing before sputum collection to reduce contamination of mycobacterial cultures

Journal of Clinical Microbiology

Volumn 49, Issue 8, 2011, Pages 3058-3060

  Peres, Renata L ^a   Palaci, Moisés ^a   Loureiro, Rafaela B ^a   Dietze, Reynaldo ^a   Johnson, John L ^b   Golub, Jonathan E ^c,d   Ruffino Netto, A ^e   Maciel, Ethel L ^a  

^a FEDERAL UNIVERSITY OF ESPÍRITO SANTO   (Brazil)

^b UNIVERSITY HOSPITALS OF CLEVELAND   (United States)

^c JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d JOHNS HOPKINS BLOOMBERG SCHOOL OF PUBLIC HEALTH   (United States)

^e UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

CETYLPYRIDINIUM SALT; CHLORHEXIDINE GLUCONATE;

ADULT; ARTICLE; BACTERIAL GROWTH; BACTERIUM CONTAMINATION; BACTERIUM CULTURE; BACTERIUM DETECTION; CONTROLLED STUDY; CULTURE MEDIUM; HUMAN; LUNG TUBERCULOSIS; MAJOR CLINICAL STUDY; MOUTH HYGIENE; MYCOBACTERIUM TUBERCULOSIS; PRIORITY JOURNAL; RANDOMIZED CONTROLLED TRIAL; SPUTUM EXAMINATION;

ADOLESCENT; ADULT; ANTI-INFECTIVE AGENTS, LOCAL; CETYLPYRIDINIUM; CHLORHEXIDINE; HUMANS; MIDDLE AGED; MOUTHWASHES; MYCOBACTERIUM TUBERCULOSIS; SPUTUM; TUBERCULOSIS; YOUNG ADULT;

EID: 79960965717     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00541-11     Document Type: Article

References (17)

1. Alisjahbana, B., et al. 2005. Better patient instruction for sputum sampling can improve microscopic tuberculosis diagnosis. Int. J. Tuberc. Lung Dis. 9:814-817.
2. Barros, V. M. R., I. Y. Ito, R. V. P. Azevedo, D. Morello, and P. A. Rosatelli. 1998. Estudo comparativo da eficiência de três métodos de anti-sepsia intrabucal na redução do número de estreptococos do sulco gengival. Rev. Odontol. Univ. Sao Paulo 12:201-206.
3. Denton, G. W. 1991. Chlorhexidine, p. 274-289. In S. S. Block (ed.), Disinfection, sterilization and preservation, 4th ed. Lea and Fibiger, Philadelphia, PA.
4. Grossman, E., G. Reiter, and O. P. Styrzenberg. 1986. Six-month study of effects of a chlorhexidine mouthrinse on gingivitis in adults. J. Periodontal Res. 343:33-43.
5. Kent, P. T., and G. P. Kubica. 1985. Public health mycobacteriology: a guide for the level III laboratory. Department of Health and Human Services, Centers for Disease Control, Atlanta, GA.
6. Khan, M. S., O. Dar, C. Sismanidis, K. Shah, and P. Godfrey-Faussett. 2007. Improvement of tuberculosis case detection and reduction of discrepancies between men and women by simple sputum-submission instructions: a pragmatic randomised controlled trial. Lancet 369:1955-1960. (Pubitemid 46874579)
7. Kudoh, S., and T. Kudoh. 1974. A simple technique for culturing tubercle bacilli. Bull. World Health Organ. 51:71-82.
8. Maciel, E. L. N., et al. 2009. Guided sputum sample collection and culture contamination rates in the diagnosis of pulmonary TB. J. Bras. Pneumol. 35:460-463.
9. McDonnell, G., and A. D. Russell. 1999. Antiseptics and disinfectants: activity, action, and resistance. Clin. Microbiol. Rev. 12:147-179.
10. Muzanye, G., K. Morgan, J. Johnson, and H. Mayanja-Kizza. 2009. Impact of mouth rinsing before sputum collection on culture contamination. Afr. Health Sci. 9:200.
11. Pardini, M., et al. 2005. Cetyl-pyridinium chloride is useful for isolation of Mycobacterium tuberculosis from sputa subjected to long-term storage. J. Clin. Microbiol. 43:442-444.
12. Peres, R. L., et al. 2009. Comparison of two concentrations of NALC-NaOH for decontamination of sputum for mycobacterial culture. Int. J. Tuberc. Lung Dis. 13:1572-1575.
13. Roldán, S., et al. 2004. Comparative effects of different chlorhexidine mouthrinse formulations on volatile sulphur compounds and salivary bacterial counts. J. Clin. Periodontol. 31:1128-1134.
14. Russell, A. D., and I. Ahonkhai. 1982. Antibacterial activity of a new thiatetracycline antibiotic, thiacycline, in comparison with tetracycline, doxycycline and minocycline. J. Antimicrob. Chemother. 9:445-449.
15. Russell, A. D., S. A. Hammond, and J. R. Morgan. 1986. Bacterial resistance to antiseptics and disinfectants. J. Hosp. Infect. Rev. 7:213-225.
16. Russell, A. D. 1996. Activity of biocides against mycobacteria. J. Appl. Bacteriol. 81:87S-101S.
17. Smithwick, R. W., C. B. Stratigos, and H. L. David. 1975. Use of cetylpyridinium chloride and sodium chloride for the decontamination of sputum specimens that are transported to the laboratory for the isolation of Mycobacterium tuberculosis. J. Clin. Microbiol. 1:411-413.