Evaluation of the ReSSQ assay in relation to the COBAS AMPLICOR CMV MONITOR test and an in-house nested PCR method for detection of cytomegalovirus DNA

Journal of Clinical Microbiology

Volumn 43, Issue 8, 2005, Pages 4057-4063

  Wirgart, Benita Zweygberg ^a   Andersson, Pia ^a   Grillner, Lena ^a  

^a KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS DNA;

ARTICLE; CONTROLLED STUDY; CYTOMEGALOVIRUS; DIAGNOSTIC ACCURACY; DIAGNOSTIC KIT; DNA ISOLATION; HUMAN; IMMUNOASSAY; LABORATORY TEST; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; VALIDATION PROCESS; VIRUS DETECTION;

CYTOMEGALOVIRUS; DNA, VIRAL; HUMANS; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC;

CUCUMBER MOSAIC VIRUS; CYTOMEGALOVIRUS;

EID: 23744432783     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.43.8.4057-4063.2005     Document Type: Article

References (19)

1. Boeckh, M., and G. Boivin. 1998. Quantitation of cytomegalovirus: methodologic aspects and clinical applications. Clin. Microbiol. Rev. 11:533-554.
2. Caliendo, A. M., K. St. George, J. Allega, A. C. Bullotta, L. Gilbane, and C. R. Rinaldo. 2002. Distinguishing cytomegalovirus (CMV) infection and disease with CMV nucleic acid tests. J. Clin. Microbiol. 40:1581-1586.
3. Demidov, V. V., V. N. Potaman, M. D. Frank-Kamenetskii, M. Egholm, O. Buchardt, S. H. Sönnichsen, and P. E. Nielsen. 1994. Stability of peptide nucleic acids in human serum and cellular extracts. Biochem. Pharmacol. 48:1310-1313.
4. Egholm, M., O. Buchardt, L. Christensen, C. Behrens, S. M. Freier, D. A. Driver, R. H. Berg, S. K. Kim, B. Nordén, and P. E. Nielsen. 1993. PNA hybridizes to complementary oligonucleotides obeying the Watson-Crick hydrogen-bonding rules. Nature 365:566-568.
5. Herrmann, B., V. C. Larsson, C. J. Rubin, F. Sund, B. M. Eriksson, J. Arvidson, Z. Yun, K. Bondesson, and J. Blomberg. 2004. Comparison of a duplex quantitative real-time PCR assay and COBAS Amplicor CMV Monitor test for detection of cytomegalovirus. J. Clin. Microbiol. 42:1909-1914.
6. Iyer, M., J. C. Norton, and D. R. Corey. 1995. Accelerated hybridization of oligonucleotides to duplex DNA. J. Biol. Chem. 270:14712-14717.
7. Khan, G., H. O. Kangro, P. J. Coates, and R. B. Heath. 1994. Inhibitory effects of urine on the polymerase chain reaction for cytomegalovirus DNA. J. Clin. Pathol. 44:360-365.
8. Najioullah, F., D. Trouvenot, and B. Lina. 2001. Development of a real-time PCR procedure including an internal control for the measurement of HCMV viral load. J. Virol. Methods 92:55-64.
9. Schuurman, R., A. M. van Loon, M. de Vos, R. Reid, P. E. Klapper, and G. M. Cleator on behalf of the European Concerted Action on Quality Control for Molecular Diagnosis. 2002. Summary of results: first EU-QCCA human cytomegalovirus proficiency panel. [Online.] http://www.qcmd.org /Index2.htm.
10. Sia, I. G., J. A. Wilson, C. M. Groettum, M. J. Espy, T. F. Smith, and C. V. Paya. 2000. Cytomegalovirus (CMV) DNA load predicts relapsing CMV infection after solid organ transplantation. J. Infect. Dis. 181:717-720.
11. Sia, I. G., J. A. Wilson, M. J. Espy, C. V. Paya, and T. F. Smith. 2000. Evaluation of the COBAS AMPLICOR CMV Monitor test for detection of viral DNA in specimens taken from patients after liver transplantation. J. Clin. Microbiol. 38:600-606.
12. Spector, S. A., R. Wong, K. Hsia, M. Pilcher, and M. J. Stempien. 1998. Plasma cytomegalovirus (CMV) DNA load predicts CMV disease and survival in AIDS patients. J. Clin. Investig. 101:497-502.
13. Spector, S. A., K. Hsia, M. Crager, M. Pilcher, S. Cabral, and M. J. Stempein. 1999. Cytomegalovirus (CMV) DNA load is an independent predictor of CMV disease and survival in advanced AIDS. J. Virol. 73:7027-7030.
14. Svanvik, N., G. Westman, W. Dongyuan, and M. Kubista. 2000. Light-Up probes: thiazole orange-conjugated peptide nucleic acid for detection of target nucleic acid in homogeneous solution. Anal. Biochem. 281:26-35.
15. Tomac, S., M. Sarkar, T. Ratilainen, P. Wittung, P. E. Nielsen, B. Nordén, and A. Gräslund. 1996. Ionic effects on the stability of peptide nucleic acid complexes. J. Am. Chem. Soc. 118:5544-5552.
16. Tong, C. Y. W., L. E. Cuevas, H. Williams, and A. Bakran. 2000. Comparison of two commercial methods for measurement of cytomegalovirus load in blood samples after renal transplantation. J. Clin. Microbiol. 38:1209-1213.
17. Yun, Z., I. Lewensohn-Fuchs, P. Ljungman, L. Ringholm, J. Jonsson, and J. Albert. 2003. A real-time TaqMan PCR for routine quantitation of cytomegalovirus DNA in crude leukocyte lysates from stem cell transplant patients. J. Virol. Methods 110:73-79.
18. Zweygberg Wirgart, B., M. Brytting, A. Linde, B. Wahren, and L. Griller. 1998. Sequence variation within three important cytomegalovirus gene regions in isolates from four different patient populations. J. Clin. Microbiol. 36:3662-3669.
19. Zweygberg Wirgart, B., K. Claesson, B.-M. Eriksson, M. Brundin, G. Tufvesson, T. Tötterman, and L. Griller. 1996. Cytomegalovirus (CMV) DNA amplification from plasma compared with CMV pp65 antigen (ppUL83) detection in leucocytes for early diagnosis of symptomatic CMV infection in kidney transplant patients. J. Clin. Diagn. Virol. 7:99-110.