Molecular detection and epidemiology of extended-spectrum beta-lactamase genes prevalent in clinical isolates of Klebsiella pneumoniae and E coli from Trinidad and Tobago

West Indian Medical Journal

Volumn 59, Issue 6, 2010, Pages 591-596

  Akpaka, P E ^a   Legall, B ^a   Padman, J ^b  

^a UNIVERSITY OF THE WEST INDIES   (Trinidad and Tobago)

^b MCMASTER UNIVERSITY   (Canada)

Author keywords

Extended spectrum beta lactamase; Molecular diagnostics; Multiplex polymerase chain reaction

Indexed keywords

AMIKACIN; CARBAPENEM; CEFTAZIDIME; CLAVULANIC ACID; EXTENDED SPECTRUM BETA LACTAMASE; AMINOGLYCOSIDE; BETA LACTAMASE; BETA LACTAMASE TEM 3; BETA-LACTAMASE TEM-3; CARBAPENEM DERIVATIVE;

ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIUM DETECTION; BACTERIUM ISOLATION; CONTROLLED STUDY; EPSILOMETER TEST; ESCHERICHIA COLI; FEMALE; HUMAN; KLEBSIELLA PNEUMONIAE; MINIMUM INHIBITORY CONCENTRATION; MOLECULAR CLONING; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; PULSED FIELD GEL ELECTROPHORESIS; TRINIDAD AND TOBAGO; DRUG EFFECT; ENZYMOLOGY; ESCHERICHIA COLI INFECTION; GENETICS; ISOLATION AND PURIFICATION; KLEBSIELLA INFECTION; MALE; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY;

AMINOGLYCOSIDES; BETA-LACTAMASES; CARBAPENEMS; ELECTROPHORESIS, GEL, PULSED-FIELD; ESCHERICHIA COLI; ESCHERICHIA COLI INFECTIONS; FEMALE; HUMANS; KLEBSIELLA INFECTIONS; KLEBSIELLA PNEUMONIAE; MALE; MICROBIAL SENSITIVITY TESTS; PHENOTYPE; POLYMERASE CHAIN REACTION; TRINIDAD AND TOBAGO;

EID: 79960941978     PISSN: 00433144     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (26)

1. Bush K, Jacoby GA, Medeiros AA. A functional classification scheme for beta-lactamases and its correlation with molecular structure. Antimicrob Agents Chemother 1995; 39: 1211-33.
2. Pfaller MA, Jones RN for the MYSTIC Study Group (Americas). MYSTIC (Meropenem Yearly Susceptibility Test Information Collection) results from the Americas: resistance implications in the treatment of serious infections. J Antimicrob Chemother 2000, 46: 25-37.
3. Goossens H, for the MYSTIC Study Group (European Centres). MYSTIC (Meropenem Yearly Susceptibility Test Information Collection) results from Europe: comparison of antibiotic susceptibilities between countries and centre types. J Antimicrob Chemother 2000, 46: 39-52.
4. Hernández JR, Martínez-Martínez L, Cantón R, Coque TM, Pascual A, and Spanish Group for Nosocomial Infections (GEIH). National Study of Escherichia coli and Klebsiella pneumoniae producing Extended-Spectrum β-lactamases in Spain. Antimicrob Agents Chemother 2005; 49: 2122-5.
5. Edelstein M, Pimkin M, Palagin I, Edelstein I, Stratchounski L. Prevalence and Molecular Epidemiology of CTX-M Extended-Spectrum B-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in Russian Hospitals. Antimicrob Agents Chemother 2003; 47: 3724-32.
6. Essack SY. Treatment options for extended-spectrum beta-lactamaseproducers. FEMS Microbial Lett 2000; 190: 181-4.
7. Knothe H, Shah P, Krcmery V, Antal M, Mitsuhashi S. Transferable resistance to cefotaxime, cefoxitin, cefamandole and cefuroxime in clinical isolates of Klebsiella pneumoniae and Serratia marcescens. Infection 1983; 11: 315-7.
8. Medeiros AA. Beta-lactamases. Br Med Bull 1984; 40: 18-27.
9. Kliebe C, Nies BBA, Meyer JF, Tolxdorff-Neutzling RM, Wiedemann B. Evolution of plasmid-coded resistance to broad-spectrum cephalosporins. Antimicrob Agents Chemother 1985; 28: 302-07.
10. Bauernfeind A, Grimm H, Schweighart S. A new plasmidic cefotaximase in a clinical isolate of Escherichia coli. Infection 1990; 18: 294-8.
11. st Century: Characterization, Epidemiology and Detection of this important Resistance threat. Clin Microbiol Rev 2001; 14: 933-51.
12. Cherian BP, Singh N, Charles W, Prabhakar P. Extended spectrum β-lactamase producing Salmonella enteritidis in Trinidad & Tobago. Emerg Infect Dis 1999; 5: 181-2.
13. Akpaka PE, Swanston WH. Occurrence and phenotypic detection of extended-spectrum β-Lactamases in clinical isolates of Klebsiella pneumoniae and Escherichia coli at a tertiary hospital in Trinidad & Tobago. Brazilian J Infect Dis 2008; 12: 516-20.
14. Clinical and Laboratory Standard Institute. Performance standards for antimicrobial susceptibility testing: sixteenth informational supplement. 2006 CLSI document M100-S16. CLSI, Wayne, Pa.
15. blaCTX-M genes in Enterobacteriaceae. APMIS 2007; 115: 1400-8.
16. Paterson DL, Hujer KM, Hujer AM, Yeiser B, Bonomo MD, the International Klebsiella study group et al. Extended spectrum blactamases in Klebsiella pneumoniae bloodstream isolates from seven countries: dominance and widespread prevalence of SHV-and CTX-Mtype b-lactamases. Antimicrob Agents Chemother 2003: 47: 3553-60.
17. Boyd DA, Tyler S, Christianson S, McGeer A, Muller MP, the Canadian nosocomial infection surveillance program, Health Canada et al. Complete nucleotide sequence of a 92-kilobase plasmid harbouring the CTX-M-15 extended-spectrum beta-lactamase involved in an outbreak in long-term-care facilities in Toronto, Canada. Antimicrob Agents Chemother 2004; 48: 3758-64.
18. Woodford N, Fagan EJ, Ellington MJ. Multiplex PCR for rapid detection of genes encoding CTX-M extended-spectrum B-lactamases. J Antimicrobial Chemotherapy 2006; 57: 154-5.
19. Kaufmann ME. Pulsed-field gel electrophoresis. Methods Mol Med 1998; 15: 17-31.
20. Tenover FC, Abreit RD, Goering RV, Michelsen PA, Murray BE, Persing DH et al. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 1995; 33: 2233-9.
21. Bonnet R. Growing group of extended-spectrum B-lactamases: the CTX-M enzymes. Antimicrob Agents Chemother 2004; 48: 1-14.
22. Komatsu M, Ikeda N, Aihara M, Nakamachi Y, Kinoshita S, Yamasaki K et al. Hospital outbreak of MEN-1-derived extended spectrum betalactamase-producing Klebsiella pneumoniae. J Infect Chemother 2001; 7: 94-101.
23. Pinto Pereira LM, Phillips M, Ramlal H, Teemul K, Prabhakar P. Third generation cephalosporin use in a tertiary hospital in Port of Spain, Trinidad: need for antibiotic policy. BMC Infect Dis 2004, 4: 59. doi 10.1186/1471-2334-4-59.
24. Gupta V, Datta P. Extended-spectrum beta-lactamases in community isolates from North India: frequency and predisposing factors. Int J Infect Dis 2007; 11: 88-9.
25. Minarini LA, Gales AC, Palazzo IC, Darini AL. Prevalence of Community-occurring Extended Spectrum beta-Lactamase-Producing Enterobacteriaceae in Brazil. Curr Microbiol 2007; 54: 335-41.
26. Pena C, Pujol M, Ardanuy C, Ricart A, Pallares R, Linares J et al. Epidemiology and successful control of a large outbreak due to Klebsiella pneumoniae producing extended-spectrum beta-lactamases. Antimicrob Agents Chemother 1998; 42: 53-8.