Development of a highly sensitive genus-specific quantitative reverse transcriptase real-time PCR assay for detection and quantitation of plasmodium by amplifying RNA and DNA of the 18S rRNA genes

Journal of Clinical Microbiology

Volumn 49, Issue 8, 2011, Pages 2946-2953

  Kamau, Edwin ^a   Tolbert, LaDonna S ^a   Kortepeter, Luke ^a   Pratt, Michael ^a   Nyakoe, Nancy ^b   Muringo, Linda ^b   Ogutu, Bernard ^b   Waitumbi, John N ^b   Ockenhouse, Christian F ^a  

^a WALTER REED ARMY INSTITUTE OF RESEARCH   (United States)

^b KENYA MEDICAL RESEARCH INSTITUTE   (Kenya)

Author keywords

[No Author keywords available]

Indexed keywords

NUCLEIC ACID; PROTOZOAL DNA; PROTOZOAL RNA;

ARTICLE; CONTROLLED STUDY; GENE AMPLIFICATION; GENUS; INTERMETHOD COMPARISON; LIMIT OF DETECTION; MICROORGANISM DETECTION; MICROSCOPY; NONHUMAN; PLASMODIUM; POPULATION DENSITY; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA 18S GENE; RNA GENE; SENSITIVITY ANALYSIS;

GENES, RRNA; HUMANS; MALARIA; MOLECULAR DIAGNOSTIC TECHNIQUES; PARASITOLOGY; PLASMODIUM; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 18S; SENSITIVITY AND SPECIFICITY;

EID: 79960937709     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00276-11     Document Type: Article

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