Synchronization of HeLa Cells

Methods in Molecular Biology

Volumn 761, Issue , 2011, Pages 151-161

  Ma, Hoi Tang ^a   Poon, Randy Y C ^a  

^a HONG KONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Hong Kong)

Author keywords

Cell cycle; cyclin; cyclin dependent kinases; flow cytometry; synchronization

Indexed keywords

AGENTS AFFECTING CELL DIVISION; CYCLINE; MEVINOLIN; NOCODAZOLE; THYMIDINE;

ARTICLE; CELL CYCLE; CELL CYCLE G1 PHASE; CELL CYCLE G2 PHASE; CELL CYCLE S PHASE; DRUG EFFECT; FLOW CYTOMETRY; HELA CELL; HUMAN; METABOLISM; MITOSIS; TIME LAPSE IMAGING;

CELL CYCLE; CYCLINS; FLOW CYTOMETRY; G1 PHASE; G2 PHASE; HELA CELLS; HUMANS; LOVASTATIN; MITOSIS; MITOSIS MODULATORS; NOCODAZOLE; S PHASE; THYMIDINE; TIME-LAPSE IMAGING;

EID: 79960937456     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-182-6_10     Document Type: Chapter

References (14)

1. Skloot, R. (2010) The immortal life of Henrietta Lacks. New York: Random House.
2. McLaughlin-Drubin, M. E., and Munger, K. (2009) Oncogenic activities of human papil-lomaviruses. Virus Res. 143, 195–208.
3. Chaudhry, M. A., Chodosh, L. A., McKenna, W. G., and Muschel, R. J. (2002) Gene expression profiling of HeLa cells in G1 or G2 phases. Oncogene 21, 1934–1942.
4. Whitfield, M. L., Sherlock, G., Saldanha, A. J., Murray, J. I., Ball, C. A., Alexander, K. E. et al. (2002) Identification of genes periodically expressed in the human cell cycle and their expression in tumors. Mol. Biol. Cell 13, 1977–2000.
5. Zhou, J. Y., Ma, W. L., Liang, S., Zeng, Y., Shi, R., Yu, H. L., et al. (2009) Analysis of microRNA expression profiles during the cell cycle in synchronized HeLa cells. BMB Rep. 42, 593–598.
6. Chen, X., Simon, E. S., Xiang, Y., Kachman, M., Andrews, P. C., and Wang, Y. (2010) Quantitative proteomics analysis of cell cycle-regulated Golgi disassem-bly and reassembly. J. Biol. Chem. 285, 7197–7207.
7. Wahl, A. F., and Donaldson, K. L. (2001) Centrifugal elutriation to obtain synchronous populations of cells. Curr. Protoc. Cell Biol. Chapter 8, Unit 8.5.
8. Banfalvi, G. (2008) Cell cycle synchronization of animal cells and nuclei by centrifugal elutriation. Nat. Protoc. 3, 663–673.
9. Vassilev, L. T., Tovar, C., Chen, S., Knezevic, D., Zhao, X., Sun, H., et al. (2006) Selective small-molecule inhibitor reveals critical mitotic functions of human CDK1. Proc. Natl. Acad. Sci. USA 103, 10660–10665.
10. Terasima, T., and Tolmach, L. J. (1963) Growth and nucleic acid synthesis in synchronously dividing populations of HeLa cells. Exp. Cell Res. 30, 344–362.
11. Keyomarsi, K., Sandoval, L., Band, V., and Pardee, A. B. (1991) Synchronization of tumor and normal cells from G1 to multiple cell cycles by lovastatin. Cancer Res. 51, 3602–3609.
12. Javanmoghadam-Kamrani, S., and Keyomarsi, K. (2008) Synchronization of the cell cycle using lovastatin. Cell Cycle 7, 2434–2440.
13. Ma, H. T., Tsang, Y. H., Marxer, M., and Poon, R. Y. C. (2009) Cyclin A2-cyclin-dependent kinase 2 cooperates with the PLK1-SCFbeta-TrCP1-EMI1-anaphase-promoting complex/cyclosome axis to promote genome reduplication in the absence of mitosis. Mol. Cell Biol. 29, 6500–6514.
14. Mikulski, S. M., Viera, A., Darzynkiewicz, Z., and Shogen, K. (1992) Synergism between a novel amphibian oocyte ribonuclease and lovastatin in inducing cytostatic and cytotoxic effects in human lung and pancreatic carcinoma cell lines. Br. J. Cancer 66, 304–310.