Measuring Inflammatory Cell Migration in the Zebrafish

Methods in Molecular Biology

Volumn 769, Issue , 2011, Pages 261-275

  Elks, Philip M ^b   Loynes, Catherine A ^b   Renshaw, Stephen A ^a  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

^b NONE

Author keywords

In vivo; Inflammation; Migration; Neutrophil; Zebrafish

Indexed keywords

GREEN FLUORESCENT PROTEIN; RECOMBINANT PROTEIN;

ANIMAL; ARTICLE; BIOSYNTHESIS; CELL TRACKING; COMPUTER PROGRAM; CONFOCAL MICROSCOPY; FIN (ORGAN); FLUORESCENCE MICROSCOPY; GENETICS; IMMUNOLOGY; LARVA; METHODOLOGY; NEUTROPHIL; NEUTROPHIL CHEMOTAXIS; PATHOLOGY; PHYSIOLOGY; TIME LAPSE IMAGING; TRANSGENIC ANIMAL; ZEBRA FISH;

ANIMAL FINS; ANIMALS; ANIMALS, GENETICALLY MODIFIED; CELL TRACKING; CHEMOTAXIS, LEUKOCYTE; GREEN FLUORESCENT PROTEINS; LARVA; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; NEUTROPHILS; RECOMBINANT PROTEINS; SOFTWARE; TIME-LAPSE IMAGING; ZEBRAFISH;

DANIO RERIO;

EID: 79960927721     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-207-6_18     Document Type: Chapter

References (16)

1. Lieschke, G.J., Oates, A.C., Crowhurst, M.O., Ward, A.C., Layton, J.E. (2001) Morphologic and functional characterization of granulocytes and macrophages in embryonic and adult zebrafish. Blood 98, 3087–96.
2. Lieschke, G.J., Currie, P.D. (2007) Animal models of human disease: zebrafish swim into view. Nat Rev Genet 8, 353–67.
3. Trede, N.S., Langenau, D.M., Traver, D., Look, A.T., Zon, L.I. (2004) The use of zebrafish to understand immunity. Immunity 20, 367–79.
4. Carradice, D., Lieschke, G.J. (2008) Zebrafish in hematology: sushi or science? Blood 111, 3331–42.
5. Renshaw, S.A., Loynes, C.A., Trushell, D.M., Elworthy, S., Ingham, P.W., Whyte, M.K. (2006) A transgenic zebrafish model of neu-trophilic inflammation. Blood 108, 3976–8.
6. Nasevicius, A., Ekker, S.C. (2000) Effective targeted gene ‘knockdown’ in zebrafish. Nat Genet 26, 216–20.
7. Savill, J.S., Wyllie, A.H., Henson, J.E., Walport, M.J., Henson, P.M., Haslett, C. (1989) Macrophage phagocytosis of aging neutrophils in inflammation. Programmed cell death in the neutrophil leads to its recognition by macrophages. J Clin Invest 83, 865–75.
8. Martin, R.T., Bartman, T. (2009) Analysis of heart valve development in larval zebrafish. Dev Dyn 238, 1796–802.
9. Brown, S.B., Tucker, C.S., Ford, C., Lee, Y., Dunbar, D.R., Mullins, J.J. (2007) Class III antiarrhythmic methanesulfonanilides inhibit leukocyte recruitment in zebrafish. J Leukoc Biol 82, 79–84.
10. Redd, M.J., Cooper, L., Wood, W., Stramer, B., Martin, P. (2004) Wound healing and inflammation: embryos reveal the way to perfect repair. Philos Trans R Soc Lond B Biol Sci 359, 777–84.
11. Herbomel, P., Thisse, B., Thisse, C. (2001) Zebrafish early macrophages colonize cephalic mesenchyme and developing brain, retina, and epidermis through a M-CSF receptor-depen-dent invasive process. Dev Biol 238, 274–88.
12. Loynes, C.A., Martin, J.S., Robertson, A., Trushell, D.M., Ingham, P.W., Whyte, M.K., Renshaw, S.A. Pivotal Advance: Pharmacological manipulation of inflammation resolution during spontaneously resolving tissue neutro-philia in the zebrafish. J Leukoc Biol 87, 203–12.
13. Elks, P.M., van Eeden, F.J., Dixon, G., Wang, X., Reyes-Aldasoro, C.C., Ingham, P.W., Whyte, M.K.B., Walmsley, S.R., and Renshaw, S.A. (2011) Activation of Hif-1alpha delays inflammation resolution by reducing neutrophil apoptosis and reverse migration in a zebrafish inflammation model. Blood. In press.
14. Nusslein-Volhard C, D.R. (2002) Zebrafish: A Practical Approach. Oxford University Press, Oxford.
15. Ellett, F., Pase, L., Hayman, J.W., Andrian-opoulos, A., and Lieschke, G.J. (2010) mpeg1 promoter transgenes direct macrophage-lineage expression in zebrafish. Blood. In press.
16. Gray, C., Loynes, C.A., Whyte, M.K.B., Crossman, D.C., Renshaw, S.A., and Chico, T.J.A. (2011) Simultaneous intravital imaging of macrophage and neutrophil behaviour during inflammation using a novel transgenic zebrafish. Thrombosis and Haemostasis 105.