Isolation of Centrosomes from Cultured Cells

Methods in Molecular Biology

Volumn 777, Issue , 2011, Pages 107-116

  Reber, Simone ^a  

^a MAX PLANCK INSTITUTE OF MOLECULAR CELL BIOLOGY AND GENETICS   (Germany)

Author keywords

Centrosome; Microtubule dynamics; Microtubules; Xenopus egg extracts; tubulin

Indexed keywords

TUBULIN;

ANIMAL; ARTICLE; CENTROSOME; CHEMISTRY; CHO CELL; CRICETULUS; DENSITY GRADIENT CENTRIFUGATION; HAMSTER; METABOLISM; MICROTUBULE; WESTERN BLOTTING; XENOPUS;

ANIMALS; BLOTTING, WESTERN; CENTRIFUGATION, DENSITY GRADIENT; CENTROSOME; CHO CELLS; CRICETINAE; CRICETULUS; MICROTUBULES; TUBULIN; XENOPUS;

EID: 79960923721     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-252-6_8     Document Type: Chapter

References (17)

1. Boveri, T (1901) Über die Natur der Centrosomen. Jena. Z. Med. Naturw. 28, 1–220.
2. Nigg EA (2006) Origins and consequences of centrosome aberrations in human cancers. Int J Cancer. 119:2717–2.
3. Wigge PA, Jensen ON, Holmes S, Soues S, Mann M and Kilmartin JV (1998) Analysis of the Saccharomyces spindle pole by matrix assisted laser desorption/ionization (MALDI) mass spectrometry. J. Cell Biol. 141:967–977.
4. Palazzo RE, Vogel JM (1999) Isolation of centrosomes from Spisula solidissima oocytes. Methods Cell Biol. 61:35–56.
5. Thompson-Coffe C, Coffe G, Schatten H, Mazia D, Schatten G. (1996) Cold-treated centrosome: Isolation of centrosomes from mitotic sea urchin eggs, production of an anti-centrosomal antibody, and novel ultrastructural imaging. Cell Motility and the Cytoskeleton 33:197–207.
6. Moritz M and Alberts BM (1999) Isolation of centrosomes from Drosophila embryos. Methods Cell Biol. 61:1–12.
7. Wiese C, Mayers JR, and Albee AJ (2009) Analysis of Centrosome Function and Microtubule Dynamics by Time-Lapse Microscopy in Xenopus Egg Extracts. In: Gavin RH (ed) Cytoskeleton Methods and Protocols, Methods in Molecular Biology, vol. 586.
8. Mitchison TJ, Kirschner MW (1986) Isolation of mammalian centrosomes Methods Enzymol. 134:261–8.
9. Bornens M, Paintrand M, Berges J, Marty MC, Karsenti E (1987) Structural and chemical characterization of isolated centrosomes. Cell Motil Cytoskeleton. 8(3):238–49.
10. Moudjou, M. and Bornens, M. (1998) Method of centrosome isolation from cultured animal cells. In: Cells, JE (ed), Cell Biology, a Laboratory Handbook, Second Edition, Academic Press Inc., San Diego, Vol. 2, pp. 111–119.
11. Bornens M, Moudjou M. (1999) Studying the composition and function of centrosomes in vertebrates. Methods Cell Biol. 61:13–34.
12. Blackburn GR, Barrau MD and dewey WC (1978) Partial purification of centrosomes from Chinese hamster ovary cells. Experimental Cell Research 113:183–187
13. Castoldi M, Popov AV (2003) Purification of brain tubulin through two cycles of polymerization-depolymerization in a high-molarity buffer. Protein Expr Purif. 32(1):83–8.
14. Hyman A, Drechsel D, Kellogg D, Salser S, Sawin K, Steffen P, Wordeman L, Mitchison T (1991) Preparation of modified tubulins. Methods Enzymol. 196:478–85.
15. Murray AW (1991) Cell cycle extracts. In: Kay BK and Peng HB (eds) Xenopus laevis: Practical uses in cell and molecular biology. Academic Press Inc., San Diego, Methods Cell Biol. Vol. 36. pp. 581–605.
16. Powers M, Evans EK, Yang J, Kornbluth S. (2001) Preparation and use of interphase Xenopus egg extracts. Curr Protoc Cell Biol; Chapter 11:Unit 11.10.
17. Kinoshita K, Arnal I, Desai A, Drechsel DN, Hyman AA. (2001) Reconstitution of physiological microtubule dynamics using purified components. Science 294(5545):1340–3.