Quantification of Transendothelial Migration Using Three-Dimensional Confocal Microscopy

Methods in Molecular Biology

Volumn 769, Issue , 2011, Pages 167-190

  Cain, Robert J ^b   d’Água, Bárbara Borda ^b   Ridley, Anne J ^a  

^a KING'S COLLEGE LONDON   (United Kingdom)

^b NONE

Author keywords

Cancer; Confocal; ECM; Endothelium; HUVEC; Imaging; Leukocyte; Quantification; Transmigration

Indexed keywords

COLLAGEN TYPE 1;

ARTICLE; CELL CULTURE; CELL MIGRATION; CHEMISTRY; COCULTURE; COMPUTER INTERFACE; CONFOCAL MICROSCOPY; CULTURE TECHNIQUE; CYTOLOGY; ENDOTHELIUM CELL; HUMAN; METHODOLOGY; PHYSIOLOGY; THREE DIMENSIONAL IMAGING; TRANSENDOTHELIAL AND TRANSEPITHELIAL MIGRATION; TUMOR CELL LINE; VASCULAR ENDOTHELIUM;

CELL CULTURE TECHNIQUES; CELL LINE, TUMOR; CELL MIGRATION ASSAYS; CELLS, CULTURED; COCULTURE TECHNIQUES; COLLAGEN TYPE I; ENDOTHELIAL CELLS; ENDOTHELIUM, VASCULAR; HUMANS; IMAGING, THREE-DIMENSIONAL; MICROSCOPY, CONFOCAL; TRANSENDOTHELIAL AND TRANSEPITHELIAL MIGRATION; USER-COMPUTER INTERFACE;

EID: 79960895254     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-207-6_12     Document Type: Chapter

References (19)

1. Engelhardt, B., and Wolburg, H. (2004) Minireview: Transendothelial migration of leukocytes: through the front door or around the side of the house?, Eur J Immunol 34, 2955–2963.
2. Millan, J., and Ridley, A. J. (2005) Rho GTPases and leucocyte-induced endothelial remodelling, Biochem J 385, 329–337.
3. Nourshargh, S., and Marelli-Berg, F. M. (2005) Transmigration through venular walls: a key regulator of leukocyte phenotype and function, Trends Immunol 26, 157–165.
4. Blankenberg, S., Barbaux, S., and Tiret, L. (2003) Adhesion molecules and atherosclerosis, Atherosclerosis 170, 191–203.
5. Tarrant, T. K., and Patel, D. D. (2006) Chemokines and leukocyte trafficking in rheu-matoid arthritis, Pathophysiology 13, 1–14.
6. Hooper, S., Marshall, J. F., and Sahai, E. (2006) Tumor cell migration in three dimensions, Methods Enzymol 406, 625–643.
7. Sabeh, F., Shimizu-Hirota, R., and Weiss, S. J. (2009) Protease-dependent versus-independent cancer cell invasion programs: three-dimensional amoeboid movement revisited, J Cell Biol 185, 11–19.
8. Sahai, E. (2007) Illuminating the metastatic process, Nat Rev Cancer 7, 737–749.
9. Vega, F. M., and Ridley, A. J. (2008) Rho GTPases in cancer cell biology, FEBS Lett 582, 2093–2101.
10. Chiang, A. C., and Massague, J. (2008) Molecular basis of metastasis, N Engl J Med 359, 2814–2823.
11. Nguyen, D. X., Bos, P. D., and Massague, J. (2009) Metastasis: from dissemination to organ-specific colonization, Nat Rev Cancer 9, 274–284.
12. Carman, C. V., Sage, P. T., Sciuto, T. E., de la Fuente, M. A., Geha, R. S., Ochs, H. D., Dvorak, H. F., Dvorak, A. M., and Springer, T. A. (2007) Transcellular diapedesis is initiated by invasive podosomes, Immunity 26, 784–797.
13. Muller, W. A. (2009) Mechanisms of transendothelial migration of leukocytes, Circ Res 105, 223–230.
14. Milan, J., Charalambous, C., Elhag, R., Chen, T. C., Li, W., Guan, S., Hofman, F. M., and Zidovetzki, R. (2006) Multiple signaling pathways are involved in endothelin-1-induced brain endothelial cell migration, Am J Physiol Cell Physiol 291, C155–164.
15. Cernuda-Morollon, E., Gharbi, S., and Millan, J. Discriminating between the paracellular and transcellular routes of diapedesis, Methods Mol Biol 616, 69–82.
16. Mierke, C. T., Zitterbart, D. P., Kollmannsberger, P., Raupach, C., Schlotzer-Schrehardt, U., Goecke, T. W., Behrens, J., and Fabry, B. (2008) Breakdown of the endothelial barrier function in tumor cell transmigration, Biophys J 94, 2832–2846.
17. Khuon, S., Liang, L., Dettman, R. W., Sporn, P. H., Wysolmerski, R. B., and Chew, T. L. (2010) Myosin light chain kinase mediates transcellular intravasation of breast cancer cells through the underlying endothelial cells: a three-dimensional FRET study, J Cell Sci 123, 431–440.
18. Roth, S. J., Carr, M. W., Rose, S. S., and Springer, T. A. (1995) Characterization of transendothelial chemotaxis of T lymphocytes, J Immunol Methods 188, 97–116.
19. Ding, Z., Xiong, K., and Issekutz, T. B. (2000) Regulation of chemokine-induced transendothelial migration of T lymphocytes by endothelial activation: differential effects on naive and memory T cells, J Leukoc Biol 67, 825–833.