A single amino acid change in the transmembrane domain of the VirB8 protein affects dimerization, interaction with VirB10 and Brucella suis virulence

FEBS Letters

Volumn 585, Issue 15, 2011, Pages 2431-2436

  Andrieux, Leslie ^a,b   Bourg, Gisèle ^a,b   Pirone, Audrey ^a,b   O'Callaghan, David ^a,b   Patey, Gilles ^a,b  

^a CHU Nîmes   (France)

^b UNIV MONTPELLIER   (France)

Author keywords

Bacterial two hybrid; Brucella; Type 4 secretion system; VirB10; VirB8

Indexed keywords

BACTERIAL PROTEIN; HETERODIMER; HOMODIMER; UNCLASSIFIED DRUG; VIRB10 PROTEIN; VIRB8 PROTEIN;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL VIRULENCE; BACTERIUM MUTANT; BRUCELLA SUIS; CONTROLLED STUDY; DIMERIZATION; NONHUMAN; PHENOTYPE; POINT MUTATION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROTEIN STABILITY; RHIZOBIUM RADIOBACTER; TYPE IV SECRETION SYSTEM; WILD TYPE;

AMINO ACID SUBSTITUTION; BRUCELLA SUIS; GENETIC VARIATION; MEMBRANE PROTEINS; PROTEIN BINDING; PROTEIN MULTIMERIZATION; RHIZOBIUM RADIOBACTER; VIRULENCE; VIRULENCE FACTORS;

AGROBACTERIUM TUMEFACIENS; BACTERIA (MICROORGANISMS); BRUCELLA; BRUCELLA MELITENSIS BIOVAR SUIS;

EID: 79960895152     PISSN: 00145793     EISSN: 18733468     Source Type: Journal    
DOI: 10.1016/j.febslet.2011.07.004     Document Type: Article

References (25)

1. Christie, P.J., Atmakuri, K., Krishnamoorthy, V., Jakubowski, S. and Cascales, E. (2005) Biogenesis, architecture, and function of bacterial type IV secretion systems. Annu. Rev. Microbiol. 59, 451-485. (Pubitemid 41507439)
2. Cascales, E. and Christie, P.J. (2003) The versatile bacterial type IV secretion systems. Nat. Rev. Microbiol. 1, 137-149.
3. O'Callaghan, D., Cazevieille, C., Allardet, S.A., Boschiroli, M.L., Bourg, G., Foulongne, V., Frutos, P., Kulakov, Y. and Ramuz, M. (1999) A homologue of the Agrobacterium tumefaciens VirB and Bordetella pertussis Ptl type IV secretion systems is essential for intracellular survival of Brucella suis. Mol. Microbiol. 33, 1210-1220. (Pubitemid 29433626)
4. Sieira, R., Comerci, D.J., Sanchez, D.O. and Ugalde, R.A. (2000) A homologue of an operon required for DNA transfer in Agrobacterium is required in Brucella abortus for virulence and intracellular multiplication. J. Bacteriol. 182, 4849-4855. (Pubitemid 30641808)
5. Boschiroli, M.L., Foulongne, V. and O'Callaghan, D. (2001) Brucellosis: a worldwide zoonosis. Curr. Opin. Microbiol. 4, 58-64. (Pubitemid 32126898)
6. Pappas, G., Akritidis, N., Bosilkovski, M. and Tsianos, E. (2005) Brucellosis. N. Engl. J. Med. 352, 2325-2336.
7. Backert, S., Fronzes, R. and Waksman, G. (2008) VirB2 and VirB5 proteins: specialized adhesins in bacterial type-IV secretion systems? Trends Microbiol. 16, 409-413.
8. Fronzes, R., Schafer, E., Wang, L., Saibil, H.R., Orlova, E.V. and Waksman, G. (2009) Structure of a type IV secretion system core complex. Science 323, 266-268.
9. Chandran, V., Fronzes, R., Duquerroy, S., Cronin, N., Navaza, J. and Waksman, G. (2009) Structure of the outer membrane complex of a type IV secretion system. Nature 462, 1011-1015.
10. Kumar, R.B., Xie, Y.H. and Das, A. (2000) Subcellular localization of the Agrobacterium tumefaciens T-DNA transport pore proteins: VirB8 is essential for the assembly of the transport pore. Mol. Microbiol. 36, 608-617. (Pubitemid 30258845)
11. Kumar, R.B. and Das, A. (2001) Functional analysis of the Agrobacterium tumefaciens T-DNA transport pore protein VirB8. J. Bacteriol. 183, 3636-3641. (Pubitemid 32510432)
12. Terradot, L., Bayliss, R., Oomen, C., Leonard, G.A., Baron, C. and Waksman, G. (2005) Structures of two core subunits of the bacterial type IV secretion system, VirB8 from Brucella suis and ComB10 from Helicobacter pylori. Proc. Natl. Acad. Sci. USA 102, 4596-4601. (Pubitemid 40397157)
13. Bailey, S., Ward, D., Middleton, R., Grossmann, J.G. and Zambryski, P.C. (2006) Agrobacterium tumefaciens VirB8 structure reveals potential protein-protein interaction sites. Proc. Natl. Acad. Sci. USA 103, 2582-2587.
14. Paschos, A., Patey, G., Sivanesan, D., Gao, C., Bayliss, R., Waksman, G., O'Callaghan, D. and Baron, C. (2006) Dimerization and interactions of Brucella suis VirB8 with VirB4 and VirB10 are required for its biological activity. Proc. Natl. Acad. Sci. USA 103, 7252-7257. (Pubitemid 43727819)
15. Patey, G., Qi, Z., Bourg, G., Baron, C. and O'Callaghan, D. (2006) Swapping of periplasmic domains between Brucella suis VirB8 and a pSB102 VirB8 homologue allows heterologous complementation. Infect. Immun. 74, 4945-4949. (Pubitemid 44157286)
16. Schneiker, S., Keller, M., Droge, M., Lanka, E., Puhler, A. and Selbitschka, W. (2001) The genetic organization and evolution of the broad host range mercury resistance plasmid pSB102 isolated from a microbial population residing in the rhizosphere of alfalfa. Nucleic Acids Res. 29, 5169-5181. (Pubitemid 34065436)
17. Bourg, G., Sube, R., O'Callaghan, D. and Patey, G. (2009) Interactions between Brucella suis VirB8 and its homolog TraJ from the plasmid pSB102 underline the dynamic nature of type IV secretion systems. J. Bacteriol. 191, 2985-2992.
18. Ho, S.N., Hunt, H.D., Horton, R.M., Pullen, J.K. and Pease, L.R. (1989) Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77, 51-59. (Pubitemid 19125653)
19. Johnson, R.M., Hecht, K. and Deber, C.M. (2007) Aromatic and cation-π interactions enhance helix-helix association in a membrane environment. Biochemistry 46, 92008-99214.
20. Boschiroli, M.L., Ouahrani-Bettache, S., Foulongne, V., Michaux-Charachon, S., Bourg, G., Allardet-Servent, A., Cazevieille, C., Liautard, J.P., Ramuz, M. and O'Callaghan, D. (2002) The Brucella suis virB operon is induced intracellularly in macrophages. Proc. Natl. Acad. Sci. USA 99, 1544-1549. (Pubitemid 34136529)
21. Waksman, G. and Fronzes, R. (2010) Molecular architecture of bacterial type IV secretion systems. Trends Biochem. Sci. 35, 691-698.
22. Baron, C., Domke, N., Beinhofer, M. and Hapfelmeier, S. (2001) Elevated temperature differentially affects virulence, VirB protein accumulation, and T-pilus formation in different Agrobacterium tumefaciens and Agrobacterium vitis strains. J. Bacteriol. 183, 6852-6861. (Pubitemid 33064199)
23. Cascales, E. and Christie, P.J. (2004) Definition of a bacterial type IV secretion pathway for a DNA substrate. Science 304, 1170-1173. (Pubitemid 38661856)
24. Vergunst, A.C., Schrammeijer, B., den-Dulk-Ras, A., de-Vlaam, C.M.T., Regensburg, T.T. and Hooykaas, P.J.J. (2000) VirB/D4-dependent protein translocation from Agrobacterium into plant cells. Science 290, 979-982.
25. Sivanesan, D. and Baron, C. (2011) The dimer interface of Agrobacterium tumefaciens VirB8 is important for type IV secretion system function, stability, and association of VirB2 with the core complex. J. Bacteriol. 193, 2097-2106.