Microbial culture and checkerboard DNA-DNA hybridization assessment of bacteria in root canals of primary teeth pre- and post-endodontic therapy with a calcium hydroxide/chlorhexidine paste

International Journal of Paediatric Dentistry

Volumn 21, Issue 5, 2011, Pages 353-360

  Ito, Izabel Yoko ^a,b   Junior, Fumio Matoba ^b   Paula Silva, Francisco Wanderley Garcia ^b   Da Silva, Léa Assed Bezerra ^b   Leonardo, Mário Roberto ^b   Nelson Filho, Paulo ^b  

^a Department of Clinical Analysis   (Brazil)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; BIOMEDICAL AND DENTAL MATERIALS; CALCIUM HYDROXIDE; CHLORHEXIDINE;

AEROBIC BACTERIUM; ANAEROBIC BACTERIUM; ANALYSIS OF VARIANCE; ARTICLE; BACTERIAL COUNT; BACTEROIDES; CHI SQUARE DISTRIBUTION; CHILD; DECIDUOUS TOOTH; DRUG EFFECT; ENDODONTICS; FEMALE; GRAM NEGATIVE BACTERIUM; HUMAN; MALE; METHODOLOGY; MICROBIOLOGY; NONPARAMETRIC TEST; NUCLEIC ACID HYBRIDIZATION; PRESCHOOL CHILD; STREPTOCOCCUS; TOOTH PERIAPICAL DISEASE; TOOTH PULP; TOOTH PULP DISEASE;

ANALYSIS OF VARIANCE; BACTERIA, AEROBIC; BACTERIA, ANAEROBIC; BACTEROIDES; CALCIUM HYDROXIDE; CHI-SQUARE DISTRIBUTION; CHILD; CHILD, PRESCHOOL; CHLORHEXIDINE; COLONY COUNT, MICROBIAL; DENTAL PULP CAVITY; DENTAL PULP NECROSIS; DNA, BACTERIAL; FEMALE; GRAM-NEGATIVE BACTERIA; HUMANS; MALE; NUCLEIC ACID HYBRIDIZATION; PERIAPICAL PERIODONTITIS; ROOT CANAL IRRIGANTS; ROOT CANAL PREPARATION; STATISTICS, NONPARAMETRIC; STREPTOCOCCUS; TOOTH, DECIDUOUS;

EID: 79960847686     PISSN: 09607439     EISSN: 1365263X     Source Type: Journal    
DOI: 10.1111/j.1365-263X.2011.01131.x     Document Type: Article

References (39)

1. Kakehashi S, Stanley HR, Fitzgerald RJ. The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats. Oral Surg Oral Med Oral Pathol 1965; 20: 340-349.
2. Nair PN, Henry S, Cano V, Vera J. Microbial status of apical root canal system of human mandibular first molars with primary apical periodontitis after "one-visit" endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2005; 99: 231-252.
3. Siqueira-Junior JF, Rôças IN. The microbiota of acute apical abscesses. J Dent Res 2009; 88: 61-65.
4. Siqueira-Junior JF, Rôças IN. Uncultivated phylotypes and newly named species associated with primary and persistent endodontic infections. J Clin Microbiol 2005; 43: 3314-3319.
5. Assed S, Ito IY, Leonardo MR, Silva LAB, Lopatin DE. Anaerobic microorganisms in root canals of human teeth with chronic apical periodontitis detected by indirect immunofluorescence. Endod Dent Traumatol 1996; 12: 66-69.
6. Onçag O, Hosgor M, Hilmioglu S, Zekioglu O, Eronat C, Burhanoglu D. Comparison of antibacterial and toxic effects of various root canal irrigants. Int Endod J 2003; 36: 423-432.
7. Siqueira-Junior JF, Rôças IN, Alves FR, Santos KR. Selected endodontic pathogens in the apical third of infected root canals: a molecular investigation. J Endod 2004; 30: 638-643.
8. Rôças IN, Siqueira-Junior JF. Occurrence of two newly named oral treponemes -Treponema parvum and Treponema putidum- in primary endodontic infections. Oral Microbiol Immunol 2005; 20: 372-375.
9. Siqueira-Junior JF, Rôças IN, Souto R, Uzeda M, Colombo AP. Actinomyces species, Streptococci, and Enterococcus faecalis in primary root canal infections. J Endod 2002; 28: 168-172.
10. Souza CA, Teles RP, Souto R, Chaves MA, Colombo AP. Endodontic therapy associated with calcium hydroxide as an intracanal dressing: microbiologic evaluation by the checkerboard DNA-DNA hybridization technique. J Endod 2005; 31: 79-83.
11. Sato T, Hoshino E, Uematsu H, Noda T. Predominant obligate anaerobes in necrotic pulps of human deciduous teeh. Microb Ecol Health Dis 1993; 6: 269-275.
12. Pazelli LC, de Freitas AC, Ito IY, de Souza-Gugelmin MC, Medeiros AS, Nelson-Filho P. Prevalência de microrganismos em canais radiculares de dentes decíduos de humanos com necrose pulpar e lesão periapical crônica. Pesqui Odontol Bras 2003; 17: 367-371.
13. Ruviére DB, Leonardo MR, da Silva LA, Ito IY, Nelson-Filho P. Assessment of the microbiota in root canals of human primary teeth by checkerboard DNA-DNA hybridization. J Dent Child 2007; 74(2): 118-123.
14. Peters LB, van Winkelhoff AJ, Buijs JF, Wesselink PR. Effects of instrumentation, irrigation and dressing with calcium hydroxide on infection in pulpless teeth with periapical bone lesions. Int Endod J 2002; 35: 13-21.
15. Zerella JA, Fouad AF, Spangberg LS. Effectiveness of a calcium hydroxide and chlorhexidine digluconate mixture as disinfectant during retreatment of failed endodontic cases. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2005; 100: 756-761.
16. Paula-Silva FW, da Silva LA, Kapila YL. Matrix metalloproteinase expression in teeth with apical periodontitis is differentially modulated by the modality of root canal treatment. J Endod 2010; 36(2): 231-237.
17. Sjogren U, Figdor D, Persson S, Sundqvist G. Influence of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis. Int Endod J 1997; 30: 297-306.
18. Tang G, Samaranayake LP, Yip HK. Molecular evaluation of residual endodontic microorganisms after instrumentation, irrigation and medication with either calcium hydroxide or Septomixine. Oral Dis 2004; 10: 389-397.
19. Oliveira LD, Leao MV, Carvalho CA et al. In vitro effects of calcium hydroxide and polymyxin B on endotoxins in root canals. J Dent 2005; 33: 107-114.
20. Rosenthal S, Spangberg L, Safavi K. Chlorhexidine substantivity in root canal dentin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2004; 98: 488-492.
21. White RR, Hays GL, Janer LR. Residual antimicrobial activity after canal irrigation with chlorhexidine. J Endod 1997; 23: 229-231.
22. Evans MD, Baumgartner JC, Khemaleelakul SU, Xia T. Efficacy of calcium hydroxide: chlorhexidine paste as an intracanal medication in bovine dentin. J Endod 2003; 29: 338-339.
23. Podbielski A, Spahr A, Haller B. Additive antimicrobial activity of calcium hydroxide and chlorhexidine on common endodontic bacterial pathogens. J Endod 2003; 29: 340-345.
24. Basrani B, Ghanem A, Tjaderhane L. Physical and chemical properties of chlorhexidine and calcium hydroxide-containing medications. J Endod 2004; 30: 413-417.
25. Dammaschke T, Schneider U, Stratmann U, Yoo JM, Schafer E. Effect of root canal dressings on the regeneration of inflamed periapical tissue. Acta Odontol Scand 2005; 63: 143-152.
26. Ferreira CM, da Silva Rosa OP, Torres SA, Ferreira FB, Bernardinelli N. Activity of endodontic antibacterial agents against selected anaerobic bacteria. Braz Dent J 2002; 13: 118-122.
27. Soares JA, Leonardo MR, da Silva LA, Tanomaru Filho M, Ito IY. Effect of rotary instrumentation and of the association of calcium hydroxide and chlorhexidine on the antisepsis of the root canal system in dogs. Braz Oral Res 2006; 20(2): 120-126.
28. Silva RA, Assed S, Nelson-Filho P, Silva LA, Consolaro A. Subcutaneous tissue response of isogenic mice to calcium hydroxide-based pastes with chlorhexidine. Braz Dent J 2009; 20(2): 99-106.
29. Silva RA, Leonardo MR, da Silva LA, Faccioli LH, de MedeirosAI. Effect of a calcium hydroxide-based paste associated to chlorhexidine on RAW 264.7 macrophage cell line culture. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008; 106(5): e44-e51.
30. Silva LA, Nelson-Filho P, Faria G, Souza-Gugelmin MC, Ito IY. Bacterial profile in primary teeth with necrotic pulp and periapical lesions. Braz Dent J 2006; 17(2): 144-148.
31. Leonardo MR, Tanomaru Filho M, Silva LA, Nelson Filho P, Bonifácio KC, Ito IY. In vivo antimicrobial activity of 2% chlorhexidine used as a root canal irrigating solution. J Endod 1999; 25(3): 167-171.
32. Leonardo MR, Guillén-Carías MG, Pécora JD, Ito IY, Silva LAB. Er:YAG laser: antimicrobial effects in the root canals of dogs' teeth with pulp necrosis and chronic periapical lesions. Photomed Laser Surg 2005; 23: 295-299.
33. Silva LA, Leonardo MR, Oliveira DS et al. Histopathological evaluation of root canal filling materials for primary teeth. Braz Dent J 2010; 21(1): 38-45.
34. Queiroz AM, Nelson-Filho P, Silva LA, Assed S, Silva RA, Ito IY. Antibacterial activity of root canal filling materials for primary teeth: zinc oxide and eugenol cement, Calen paste thickened with zinc oxide, Sealapex and EndoREZ. Braz Dent J 2009; 20(4): 290-296.
35. Siqueira JF Jr, Rôças IN. PCR-based identification of Treponema maltophilum, T amylovorum, T medium, and T lecithinolyticum in primary root canal infections. Arch Oral Biol 2003; 48: 495-502.
36. Silva LA, Fernandes PM, da Silva RA, Nelson-Filho P, Rocha LB, Rossi MA. SEM Study of apical morphological alterations in primary teeth with vital and necrotic pulps. Ultrastruct Pathol 2009; 33(5): 183-188.
37. Rocha CT, Rossi MA, Leonardo MR, Rocha LB, Nelson-Filho P, Silva LA. Biofilm on the apical region of roots in primary teeth with vital and necrotic pulps with or without radiographically evident apical pathosis. Int Endod J 2008; 41(8): 664-669.
38. Sjögren U, Figdor D, Spångberg L, Sundqvist G. The antimicrobial effect of calcium hydroxide as a short-term intracanal dressing. Int Endod J 1991; 24: 119-125.
39. Nerwich A, Figdor D, Messer HH. pH changes in root dentin over a 4-week period following root canal dressing with calcium hydroxide. J Endod 1993; 19(6): 302-306.