Functional molecular morphology of anterior pituitary cells, from hormone production to intracellular transport and secretion

Medical Molecular Morphology

Volumn 44, Issue 2, 2011, Pages 63-70

  Matsuno, Akira ^a,b   Mizutani, Akiko ^a   Okinaga, Hiroko ^b   Takano, Koji ^c   Yamada, So ^a   Yamada, Shoko M ^a   Nakaguchi, Hiroshi ^a   Hoya, Katsumi ^a   Murakami, Mineko ^a   Takeuchi, Masato ^d   Sugaya, Mutsumi ^d   Itoh, Johbu ^e   Takekoshi, Susumu ^e   Osamura, R Yoshiyuki ^f  

^a TEIKYO UNIVERSITY CHIBA MEDICAL CENTER   (Japan)

^b TEIKYO HEISEI UNIVERSITY   (Japan)

^c UNIVERSITY OF TOKYO HOSPITAL   (Japan)

^d TEIKYO UNIVERSITY   (Japan)

^e TOKAI UNIVERSITY   (Japan)

^f INTERNATIONAL UNIVERSITY OF HEALTH AND WELFARE   (Japan)

Author keywords

Enhanced yellow fluorescein protein; Intracellular transport; mRNA; Pituitary hormone; rab3B; Secretion; SNARE protein

Indexed keywords

DIAMINOBENZIDINE; MESSENGER RNA; N ETHYLMALEIMIDE SENSITIVE FACTOR; PROLACTIN; SNARE PROTEIN; SOMATOSTATIN; SYNAPTOSOMAL ASSOCIATED PROTEIN 25;

ELECTRON MICROSCOPY; GROWTH HORMONE RELEASE; HORMONE SYNTHESIS; HYPOPHYSIS CELL; HYPOPHYSIS FUNCTION; IMMUNOHISTOCHEMISTRY; PRIORITY JOURNAL; REVIEW;

ANIMALS; BACTERIAL PROTEINS; BIOLOGICAL TRANSPORT; CELL LINE; EXOCYTOSIS; GROWTH HORMONE; HUMANS; IMAGING, THREE-DIMENSIONAL; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; LUMINESCENT PROTEINS; MICROSCOPY, CONFOCAL; MICROSCOPY, ELECTRON; PITUITARY GLAND; QA-SNARE PROTEINS; QUANTUM DOTS; RAB3 GTP-BINDING PROTEINS; RATS; RNA, MESSENGER; SYNAPTOSOMAL-ASSOCIATED PROTEIN 25;

EID: 79960595164     PISSN: 18601480     EISSN: 18601499     Source Type: Journal    
DOI: 10.1007/s00795-011-0545-4     Document Type: Review

References (66)

1. A. Matsuno H. Utsunomiya Y. Ohsugi S. Takekoshi N. Sanno R.Y. Osamura K. Nagao A. Tamura T. Nagashima 1996 Simultane ous ultrastructural identification of growth hormone and its messenger ribonucleic acid using combined immunohistochemistry and non-radioisotopic in situ hybridization: a technical note Histochem J 28 703 707 8950599 10.1007/BF02409007 1:STN:280: DyaK2s7gslyktg%3D%3D (Pubitemid 26390866)
2. A. Matsuno Y. Ohsugi H. Utsunomiya S. Takekoshi S. Munakata K. Nagao R.Y. Osamura A. Tamura T. Nagashima 1998 An improved ultrastructural double-staining method of rat growth hormone and its mRNA using LR White resin: a technical note Histochem J 30 105 109 10192551 10.1023/A:1003283016889 1:CAS:528:DyaK1cXisVCns7g%3D (Pubitemid 28150191)
3. A. Matsuno T. Nagashima R.Y. Osamura K. Watanabe 1998 Application of ultrastructural in situ hybridization combined with immunohistochemistry to pathophysiological studies of pituitary cell: technical review Acta Histochem Cytochem 31 259 265 (Pubitemid 28490474)
4. A. Matsuno T. Nagashima S. Takekoshi H. Utsunomiya N. Sanno R.Y. Osamura K. Watanabe A. Tamura A. Teramoto 1998 Ultrastructural simultaneous identification of growth hormone and its messenger ribonucleic acid Endocr J 45 suppl S101 S104 9790240 10.1507/endocrj.45.Suppl-S101 1:CAS:528: DyaK1cXks1WksL8%3D
5. A. Matsuno J. Itoh R.Y. Osamura K. Watanabe T. Nagashima 1999 Electron microscopic and confocal laser scanning microscopic observation of subcellular organelles and pituitary hormone mRNA: application of ultrastructural in situ hybridization and immunohistochemistry to the pathophysiological studies of pituitary cells Endocr Pathol 10 199 211 12114700 10.1007/BF02738881 1:CAS:528:DyaK1MXotVCitrc%3D
6. A. Matsuno T. Nagashima Y. Ohsugi H. Utsunomiya S. Takekoshi S. Munakata K. Nagao R.Y. Osamura K. Watanabe 2000 Electron microscopic observation of intracellular expression of mRNA and its protein product: technical review on ultrastructural in situ hybridization and its combination with immunohistochemistry Histol Histopathol 15 261 268 10668215 1:CAS:528: DC%2BD3cXhs1yiurg%3D (Pubitemid 30044309)
7. R.Y. Osamura Y. Itoh A. Matsuno 2000 Application of plastic embedding to electron microscopic immunocytochemistry and in situ hybridization in observations of production and secretion of peptide hormones J Histochem Cytochem 48 885 892 10858265 10.1177/002215540004800701 1:CAS:528: DC%2BD3cXksFyjt7g%3D
8. R.Y. Osamura S. Tahara R. Kurotani N. Sanno A. Matsuno A. Teramoto 2000 Contributions of immunohistochemistry and in situ hybridization to the functional analysis of pituitary adenomas J Histochem Cytochem 48 445 458 10727285 10.1177/002215540004800401 1:CAS:528:DC%2BD3cXisVyms70%3D (Pubitemid 30210347)
9. D.J. Arndt-Jovin M. Robert-Nicoud S.J. Kaufman T.M. Jovin 1985 Fluorescence digital imaging microscopy in cell biology Science 230 247 256 4048934 10.1126/science.4048934 1:STN:280:DyaL28%2FhvVyhtA%3D%3D (Pubitemid 16225655)
10. D.J. Arndt-Jovin M. Robert-Nicoud T.M. Jovin 1990 Probing DNA structure and function with a multi-wavelength fluorescence confocal laser microscope J Microsc 157 61 72 2105397 1:CAS:528:DyaK3cXhvFCksrg%3D (Pubitemid 20261950)
11. J.G. Bauman J.A. Bayer H. van Dekken 1990 Fluorescent in-situ hybridization to detect cellular RNA by flow cytometry and confocal microscopy J Microsc 157 73 81 1688948 1:CAS:528:DyaK3cXhvFCltr8%3D (Pubitemid 20261951)
12. P. Hozak J.T. Novak K. Smetana 1989 Three-dimensional reconstructions of nucleolus-organizing regions in PHA-stimulated human lymphocytes Biol Cell 66 225 233 2605368 10.1016/0248-4900(89)90175-5 1:STN:280:DyaK3c7gsF2isQ%3D%3D (Pubitemid 19245709)
13. J. Itoh R.Y. Osamura K. Watanabe 1992 Subcellular visualization of light microscopic specimens by laser scanning microscopy and computer analysis: a new application of image analysis J Histochem Cytochem 40 955 967 1607644 10.1177/40.7.1607644 1:CAS:528:DyaK38Xks1Grs7g%3D
14. J. Itoh N. Sanno A. Matsuno Y. Itoh K. Watanabe R.Y. Osamura 1997 Application of confocal laser scanning microscopy (CLSM) to visualize prolactin (PRL) and PRL mRNA in the normal and estrogen-treated rat pituitary glands using non-fluorescent probes Microsc Res Tech 39 157 167 9361267 10.1002/(SICI)1097- 0029(19971015)39:2<157::AID-JEMT7>3.0.CO;2-I 1:CAS:528:DyaK2sXntlGjsrc%3D (Pubitemid 27475457)
15. J. Itoh A. Matsuno Y. Yamamoto K. Kawai A. Serizawa K. Watanabe Y. Itoh R.Y. Osamura 2001 Confocal laser scanning microscopic imaging of subcellular organelles, mRNA, protein products, and the microvessel environment Acta Histochem Cytochem 34 285 297 10.1267/ahc.34.285 1:CAS:528:DC%2BD3MXotlCrsb8%3D (Pubitemid 32930776)
16. E. Michel J.A. Parsons 1990 Histochemical and immunocytochemical localization of prolactin receptors on Nb2 lymphoma cells: applications of confocal microscopy J Histochem Cytochem 38 965 973 2355177 10.1177/38.7.2355177 1:CAS:528:DyaK3cXltFWqsLw%3D (Pubitemid 20186201)
17. J.M. Robinson B.E. Batten 1989 Detection of diaminobenzidine reactions using scanning laser confocal reflectance microscopy J Histochem Cytochem 37 1761 1765 2555410 10.1177/37.12.2555410 1:CAS:528:DyaK3cXjt1yjuw%3D%3D (Pubitemid 20002739)
18. T. Takamatsu S. Fujita 1988 Microscopic tomography by laser scanning microscopy and its three-dimensional reconstruction J Microsc 149 167 174 3398041 1:STN:280:DyaL1c3os1Oiuw%3D%3D
19. W. Tao R.J. Walter M.W. Berns 1988 Laser-transected microtubules exhibit individuality of regrowth, however most free new ends of the microtubules are stable J Cell Biol 107 1025 1035 3047148 10.1083/jcb.107.3.1025 1:STN:280:DyaL1czjtFSrug%3D%3D
20. J.G. White W.B. Amos M. Fordham 1987 An evaluation of confocal versus conventional imaging of biological structures by fluorescence light microscopy J Cell Biol 105 41 48 3112165 10.1083/jcb.105.1.41 1:STN:280:DyaL2s3otVekug%3D%3D
21. M. Bruchez Jr M. Moronne P. Gin S. Weiss A.P. Alivisatos 1998 Semiconductor nanocrystals as fluorescent biological labels Science 281 2013 2016 9748157 10.1126/science.281.5385.2013 1:CAS:528:DyaK1cXmtlKgurw%3D (Pubitemid 28475485)
22. C. Wang M. Shim P. Guyot-Sionnest 2001 Electrochromic nanocrystal quantum dots Science 291 2390 2392 11264530 10.1126/science.291.5512.2390 1:CAS:528:DC%2BD3MXit1KntLc%3D (Pubitemid 32231796)
23. W.C. Chan D.J. Maxwell X. Gao R.E. Bailey M. Han S. Nie 2002 Luminescent quantum dots for multiplexed biological detection and imaging Curr Opin Biotechnol 13 40 46 11849956 10.1016/S0958-1669(02)00282-3 1:CAS:528: DC%2BD38XhtF2itr0%3D (Pubitemid 34146546)
24. X. Gao W.C. Chan S. Nie 2002 Quantum-dot nanocrystals for ultrasensitive biological labeling and multicolor optical encoding J Biomed Opt 7 532 537 12421118 10.1117/1.1506706 1:CAS:528:DC%2BD38XotFyqsLk%3D (Pubitemid 35459933)
25. X. Gao S. Nie 2003 Molecular profiling of single cells and tissue specimens with quantum dots Trends Biotechnol 21 371 373 12948664 10.1016/S0167-7799(03)00209-9 1:CAS:528:DC%2BD3sXms1yhtrY%3D (Pubitemid 37025770)
26. M. Han X. Gao J.Z. Su S. Nie 2001 Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules Nat Biotechnol 19 631 635 11433273 10.1038/90228 1:CAS:528:DC%2BD3MXkvFGjtrg%3D (Pubitemid 32625432)
27. S. Pathak S.K. Choi N. Arnheim M.E. Thompson 2001 Hydroxylated quantum dots as luminescent probes for in situ hybridization J Am Chem Soc 123 4103 4104 11457171 10.1021/ja0058334 1:CAS:528:DC%2BD3MXisVOrsbs%3D (Pubitemid 32899504)
28. Y. Xiao P.E. Barker 2004 Semiconductor nanocrystal probes for human metaphase chromosomes Nucleic Acids Res 32 e28 14960711 10.1093/nar/gnh024
29. A. Matsuno J. Itoh S. Takekoshi T. Nagashima R.Y. Osamura 2005 Three-dimensional imagings of the intracellular localization of growth hormone and prolactin and their mRNA using nanocrystal (Quantum dot) and confocal laser scanning microscopy techniques J Histochem Cytochem 53 833 838 15995141 10.1369/jhc.4A6577.2005 1:CAS:528:DC%2BD2MXlvVWisbw%3D (Pubitemid 40923458)
30. A. Matsuno J. Itoh S. Takekoshi T. Nagashima R.Y. Osamura 2005 Two- or three- dimensional imagings of simultaneous visualization of rat pituitary hormone and its mRNA: comparison between electron microscopy and confocal laser scanning microscopy with semiconductor nanocrystals (Quantum dots) Acta Histochem Cytochem 38 253 256 10.1267/ahc.38.253 1:CAS:528:DC%2BD2MXhtFWnsb%2FF (Pubitemid 41380959)
31. A. Matsuno J. Itoh S. Takekoshi Y. Itoh Y. Ohsugi H. Katayama T. Nagashima R.Y. Osamura 2003 Dynamics of subcellular organelles, growth hormone, rab3b, SNAP-25, and syntaxin in rat pituitary cells caused by growth hormone releasing hormone and somatostatin Microsc Res Tech 62 232 239 14506689 10.1002/jemt.10364 1:CAS:528:DC%2BD3sXosF2mtbs%3D (Pubitemid 37169755)
32. A. Matsuno J. Itoh S. Takekoshi T. Nagashima R.Y. Osamura 2003 Functional and morphological analyses of rab proteins and the soluble N-ethylmaleimide- sensitive factor attachment protein receptor (SNARE) system in the secretion of pituitary hormones Acta Histochem Cytochem 36 501 506 10.1267/ahc.36.501 1:CAS:528:DC%2BD2cXhtlGntrs%3D (Pubitemid 38186115)
33. A. Matsuno A. Mizutani J. Itoh S. Takekoshi T. Nagashima H. Okinaga K. Takano R.Y. Osamura 2005 Establishment of stable GH3 cell line expressing enhanced yellow fluorescein protein-growth hormone fusion protein J Histochem Cytochem 53 1177 1180 15923356 10.1369/jhc.5B6708.2005 1:CAS:528: DC%2BD2MXhtVersbrK (Pubitemid 41262987)
34. A. Matsuno J. Itoh A. Mizutani S. Takekoshi R.Y. Osamura H. Okinaga F. Ide S. Miyawaki T. Uno S. Asano J. Tanaka H. Nakaguchi M. Sasaki M. Murakami 2008 Co-transfection of EYFP-GH and ECFP-rab3B in an experimental pituitary GH3 cell: a role of rab3B in secretion of GH through porosome Folia Histochem Cytobiol 46 419 421 19141391 10.2478/v10042-008-0069-5 1:CAS:528: DC%2BC3cXhtVSlurbL
35. T.D. Lacoste X. Michalet F. Pinaud D.S. Chemla A.P. Alivisatos S. Weiss 2000 Ultrahigh-resolution multicolor colocalization of single fluorescent probes Proc Natl Acad Sci U S A 97 9461 9466 10931959 10.1073/pnas.170286097 1:CAS:528:DC%2BD3cXmtVens7k%3D (Pubitemid 30650797)
36. X. Michalet F. Pinaud T.D. Lacoste M. Dahan M.P. Bruchez A.P. Alivisatos S. Weiss 2001 Properties of fluorescent semiconductor nanocrystals and their application to biological labeling Single Mol 4 261 276 10.1002/1438- 5171(200112)2:4<261::AID-SIMO261>3.0.CO;2-P (Pubitemid 34065866)
37. A. Matsuno J. Itoh Y. Itoh R.Y. Osamura H. Katayama T. Nagashima 2001 Histopathological analyses of silent pituitary somatotroph adenomas using immunohistochemistry, in situ hybridization and confocal laser scanning microscopic observation Pathol Res Pract 197 13 20 11209812 10.1078/0344-0338- 00003 1:STN:280:DC%2BD3M7lsFOltw%3D%3D (Pubitemid 32110895)
38. Matsuno A, Itoh J, Nagashima T, Osamura RY, Watanabe K (2001) PROTOCOLS 10: confocal laser scanning microscopy. In: Lloyd RV (ed) Morphology methods: cell and molecular biology techniques. Humana Press, Totowa, NJ, pp 165-180
39. J. Itoh K. Yasumura T. Takeshita H. Ishikawa H. Kobayashi K. Ogawa K. Kawai A. Serizawa R.Y. Osamura 2000 Three-dimensional imaging of tumor angiogenesis Anal Quant Cytol Histol 22 85 90 10696466 1:STN:280: DC%2BD3c7mtFCmtA%3D%3D (Pubitemid 30106512)
40. J. Itoh K. Kawai A. Serizawa K. Yasumura K. Ogawa R.Y. Osamura 2000 A new approach to three-dimensional reconstructed imaging of hormone-secreting cells and their microvessel environments in rat pituitary glands by confocal laser scanning microscopy J Histochem Cytochem 48 569 578 10727298 10.1177/002215540004800414 1:CAS:528:DC%2BD3cXisVymsLg%3D
41. J. Itoh K. Kawai A. Serizawa Y. Yamamoto K. Ozawa A. Matsuno K. Watanabe R.Y. Osamura 2001 Three-dimensional imaging of hormone-secreting cells and their microvessel environments in estrogen-induced prolactinoma of the rat pituitary gland by confocal laser scanning microscopy Appl Immunohistochem Mol Morphol (AIMM) 9 364 370 10.1097/00022744-200112000-00013 1:STN:280: DC%2BD38%2FjtFOmsw%3D%3D (Pubitemid 33098148)
42. J. Itoh K. Yasumura K. Ogawa K. Kawai A. Serizawa Y. Yamamoto R.Y. Osamura 2003 Three-dimensional (3D) imaging of tumor angiogenesis and its inhibition: evaluation of tumor vasculartargeting agent efficacy in the DMBA-induced rat breast cancer model by confocal laser scanning microscopy (CLSM) Acta Histochem Cytochem 36 27 36 10.1267/ahc.36.27 (Pubitemid 36548488)
43. T. Noda S. Kaidzu M. Kikuchi T. Yashiro 2001 Topographic affinities of hormone-producing cells in the rat anterior pituitary gland Acta Histochem Cytochem 34 313 319 10.1267/ahc.34.313 (Pubitemid 33030599)
44. J.M. Robinson B.E. Batten 1989 Detection of diaminobenzidine reactions using scanning laser confocal reflectance microscopy J Histochem Cytochem 37 1761 1765 2555410 10.1177/37.12.2555410 1:CAS:528:DyaK3cXjt1yjuw%3D%3D (Pubitemid 20002739)
45. R. Baba M. Yamami Y. Sakuma M. Fujita S. Fujimoto 2005 Relationship between glucose transporter and changes in the absorptive system in small intestinal absorptive cells during the weaning process Med Mol Morphol 38 47 53 16158180 10.1007/s00795-004-0275-y (Pubitemid 351543892)
46. T. Matsuoka M. Kobayashi T. Sugimoto K. Araki 2005 An immunocytochemical study of regeneration of gastric epithelia in rat experimental ulcers Med Mol Morphol 38 233 242 16378232 10.1007/s00795-005-0297-0 (Pubitemid 351543860)
47. R.Y. Osamura N. Egashira M. Yamazaki S. Miyai S. Takekoshi H. Kajiwara N. Kumai S. Umemura M. Yasuda N. Sanno A. Teramoto 2003 Mechanisms for production and secretion of hormones in physiologic and pathologic conditions Acta Histochem Cytochem 36 99 103 10.1267/ahc.36.99 1:CAS:528:DC%2BD3sXjvVSns7k%3D (Pubitemid 36554126)
48. P.M. Lledo P. Vernier J.D. Vincent W.T. Mason R. Zorec 1993 Inhibition of Rab3B expression attenuates Ca(2+)-dependent exocytosis in rat anterior pituitary cells Nature (Lond) 364 540 544 10.1038/364540a0 1:CAS:528: DyaK3sXlsFKrsLg%3D (Pubitemid 23273101)
49. A. Mizoguchi 1994 Rab3A-RabGDI-Rabphilin-3A system regulating membrane fusion machinery in the synapse and the growth cone Acta Histochem Cytochem 27 117 126 1:CAS:528:DyaK2MXosVWnsw%3D%3D (Pubitemid 2136297)
50. K. Tasaka N. Masumoto J. Mizuki Y. Ikebuchi M. Ohmichi H. Kurachi A. Miyake Y. Murata 1998 Rab3B is essential for GnRHinduced gonadotrophin release from anterior pituitary cells J Endocrinol 157 267 274 9659290 10.1677/joe.0.1570267 1:CAS:528:DyaK1cXkvV2jsr4%3D (Pubitemid 28238030)
51. S. Tahara N. Sanno A. Teramoto R.Y. Osamura 1999 Expression of Rab3, a Ras-related GTP-binding protein, in human nontumorous pituitaries and pituitary adenomas Mod Pathol 12 627 634 10392640 1:STN:280:DyaK1MzhvFyisw%3D%3D (Pubitemid 29279717)
52. D.T. Hess T.M. Slater M.C. Wilson J.H. Skene 1992 The 25 kDa synaptosomal-associated protein SNAP-25 is the major methioninerich polypeptide in rapid axonal transport and a major substrate for palmitoylation in adult CNS J Neurosci 12 4634 4641 1281490 1:CAS:528:DyaK3sXls1ygsQ%3D%3D
53. G.A. Oyler G.A. Higgins R.A. Hart E. Battenberg M. Billingsley F.E. Bloom M.C. Wilson 1989 The identification of a novel synaptosomal-associated protein, SNAP-25, differentially expressed by neuronal subpopulations J Cell Biol 109 3039 3052 2592413 10.1083/jcb.109.6.3039 1:CAS:528:DyaK3cXhvFGlsLo%3D (Pubitemid 20011928)
54. M.K. Bennett N. Calakos R.H. Scheller 1992 Syntaxin: a synaptic protein implicated in docking of synaptic vesicles at presynaptic active zones Science 257 255 259 1321498 10.1126/science.1321498 1:CAS:528:DyaK3sXisFWrurk%3D
55. N. Calakos M.K. Bennett K.E. Peterson R.H. Scheller 1994 Protein-protein interactions contributing to the specificity of intracellular vesicular trafficking Science 263 1146 1149 8108733 10.1126/science.8108733 1:CAS:528:DyaK2cXis1eqsbc%3D (Pubitemid 24105962)
56. T. Sollner M.K. Bennett S.W. Whiteheart R.H. Scheller J.E. Rothman 1993 A protein assembly-disassembly pathway in vitro that may correspond to sequential steps of synaptic vesicle docking, activation, and fusion Cell 75 409 418 8221884 10.1016/0092-8674(93)90376-2 1:CAS:528:DyaK3sXms1Ols7o%3D (Pubitemid 23335067)
57. T. Sollner S.W. Whiteheart M. Brunner H. Erdjument-Bromage S. Geromanos P. Tempst J.E. Rothman 1993 SNAP receptors implicated in vesicle targeting and fusion Nature (Lond) 362 318 324 10.1038/362318a0 1:CAS:528:DyaK3sXisFSjtLo%3D (Pubitemid 23101303)
58. E.P. Garcia E. Gatti M. Butler J. Burton P. De Camilli 1994 A rat brain Sec1 homologue related to Rop and UNC18 interacts with syntaxin Proc Natl Acad Sci U S A 91 2003 2007 8134339 10.1073/pnas.91.6.2003 1:CAS:528: DyaK2cXkt12nsLo%3D (Pubitemid 24092030)
59. Y. Hata C.A. Slaughter T.C. Sudhof 1993 Synaptic vesicle fusion complex contains unc-18 homologue bound to syntaxin Nature (Lond) 366 347 351 10.1038/366347a0 1:CAS:528:DyaK2cXpvFOiuw%3D%3D (Pubitemid 23349362)
60. J. Pevsner S.C. Hsu R.H. Scheller 1994 n-Sec1: a neural-specific syntaxin-binding protein Proc Natl Acad Sci U S A 91 1445 1449 8108429 10.1073/pnas.91.4.1445 1:CAS:528:DyaK2cXitlymtLk%3D (Pubitemid 24059029)
61. J. Pevsner S.C. Hsu J.E. Braun N. Calakos A.E. Ting M.K. Bennett R.H. Scheller 1994 Specificity and regulation of a synaptic vesicle docking complex Neuron 13 353 361 8060616 10.1016/0896-6273(94)90352-2 1:CAS:528: DyaK2MXlvVyjtg%3D%3D (Pubitemid 24275093)
62. G. Jacobsson B. Meister 1996 Molecular components of the exocytotic machinery in the rat pituitary gland Endocrinology 137 5344 5356 8940356 10.1210/en.137.12.5344 1:CAS:528:DyaK28Xnt1Gmtbc%3D (Pubitemid 26423095)
63. E. Salinas J.L. Quintanar J.A. Reig 1999 Immunohistochemical study of Syntaxin-1 and SNAP-25 in the pituitaries of mouse, guinea pig and cat Acta Physiol Pharmacol Ther Latinoam 49 61 64 10797841 1:STN:280: DC%2BD3c3lvVWktA%3D%3D
64. J.L. Quintanar E. Salinas 2002 Effect of hypothyroidism on synaptosomal-associated protein of 25 kDa and syntaxin-1 expression in adenohypophyses of rat J Endocrinol Invest 25 754 758 12398231 1:CAS:528:DC%2BD38Xos1Wgtb8%3D (Pubitemid 35173946)
65. R.Y. Tsien 1998 The green fluorescent protein Annu Rev Biochem 67 509 544 9759496 10.1146/annurev.biochem.67.1.509 1:CAS:528:DyaK1cXlsFOmsb8%3D (Pubitemid 28411137)
66. C. Magoulas L. McGuinness N. Balthasar D.F. Carmignac A.K. Sesay K.E. Mathers H. Christian L. Candeil X. Bonnefont P. Mollard I.C.A.F. Robinson 2000 A secreted fluorescent reporter targeted to pituitary growth hormone cells in transgenic mice Endocrinology 141 4681 4689 11108283 10.1210/en.141.12.4681 1:CAS:528:DC%2BD3cXosVSqtro%3D (Pubitemid 32055148)