Detection, discrimination and absolute quantitation of Tomato spotted wilt virus isolates using real time RT-PCR with TaqMan ®MGB probes

Journal of Virological Methods

Volumn 176, Issue 1-2, 2011, Pages 32-37

  Debreczeni, D E ^a   Ruiz Ruiz, S ^a,d   Aramburu, J ^b   Lopez C ^c   Belliure, B ^a,e   Galipienso, L ^b   Soler, S ^c   Rubio, L ^a  

^a INSTITUTO VALENCIANO DE INVESTIGACIONES AGRARIAS IVIA   (Spain)

^b IRTA   (Spain)

^c UNIVERSITAT POLITÈCNICA DE VALÈNCIA   (Spain)

^d Universitat Politècnica de València   (Spain)

^e UNIVERSITY OF ALICANTE   (Spain)

Author keywords

Diagnosis; Quantitative RT PCR; Tospovirus; TSWV

Indexed keywords

VIRUS RNA;

ARTICLE; CONTROLLED STUDY; DATURA; DISEASE CARRIER; FRANKLINIELLA OCCIDENTALIS; GENETIC CONSERVATION; GENETIC VARIABILITY; GENOMIC M SEGMENT; GENOTYPE; MIXED INFECTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PEPPER; PRIORITY JOURNAL; QUANTITATIVE ASSAY; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA TRANSCRIPTION; SEQUENCE ALIGNMENT; TOMATO; TOMATO SPOTTED WILT; TOSPOVIRUS; VIRUS DETECTION; VIRUS GENOME; VIRUS ISOLATION;

ANIMALS; CAPSICUM; DATURA; DNA PROBES; GENOTYPE; INSECT VECTORS; INSECTS; LYCOPERSICON ESCULENTUM; PLANT DISEASES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; TAQ POLYMERASE; TOSPOVIRUS;

FRANKLINIELLA OCCIDENTALIS; TOMATO SPOTTED WILT VIRUS; TOSPOVIRUS;

EID: 79960582119     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2011.05.027     Document Type: Article

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