메뉴 건너뛰기




Volumn 145, Issue 2, 2007, Pages 96-105

A real-time RT-PCR assay for detection and absolute quantitation of Citrus tristeza virus in different plant tissues

Author keywords

CTV; Melting curve analysis; Mild isolates; Severe isolates; Standard curve; SYBR Green

Indexed keywords

GENOMIC RNA;

EID: 34548294734     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2007.05.011     Document Type: Article
Times cited : (85)

References (68)
  • 1
    • 34547699092 scopus 로고    scopus 로고
    • Class prediction of closely related plant varieties using gene expression profiling
    • Ancillo G., Gadea J., Forment J., Guerri J., and Navarro L. Class prediction of closely related plant varieties using gene expression profiling. J. Exp. Bot. 58 (2007) 1927-1933
    • (2007) J. Exp. Bot. , vol.58 , pp. 1927-1933
    • Ancillo, G.1    Gadea, J.2    Forment, J.3    Guerri, J.4    Navarro, L.5
  • 2
    • 0002071576 scopus 로고
    • Influencia del sustrato y de la fertilización en el crecimiento de plantas de lima Mejicana (Citrus aurantifolia (Christm.) Swing) cultivadas en invernadero
    • Arregui J.M., Ballester J.F., Pina J.A., and Navarro L. Influencia del sustrato y de la fertilización en el crecimiento de plantas de lima Mejicana (Citrus aurantifolia (Christm.) Swing) cultivadas en invernadero. INIA/Ser. Agríc. 19 (1982) 61-82
    • (1982) INIA/Ser. Agríc. , vol.19 , pp. 61-82
    • Arregui, J.M.1    Ballester, J.F.2    Pina, J.A.3    Navarro, L.4
  • 3
    • 0344334028 scopus 로고    scopus 로고
    • New defective RNAs from Citrus tristeza virus: evidence for a replicase-driven template switching mechanism in their generation
    • Ayllón M.A., López C., Navas-Castillo J., Mawassi M., Dawson W.O., Guerri J., Flores R., and Moreno P. New defective RNAs from Citrus tristeza virus: evidence for a replicase-driven template switching mechanism in their generation. J. Gen. Virol. 80 (1999) 817-821
    • (1999) J. Gen. Virol. , vol.80 , pp. 817-821
    • Ayllón, M.A.1    López, C.2    Navas-Castillo, J.3    Mawassi, M.4    Dawson, W.O.5    Guerri, J.6    Flores, R.7    Moreno, P.8
  • 4
    • 0034745326 scopus 로고    scopus 로고
    • Polymorphism of the 5′ terminal region of Citrus tristeza virus (CTV) RNA: incidence of three sequence types in isolates of different origin and pathogenicity
    • Ayllón M.A., López C., Navas-Castillo J., Garnsey S.M., Guerri J., Flores R., and Moreno P. Polymorphism of the 5′ terminal region of Citrus tristeza virus (CTV) RNA: incidence of three sequence types in isolates of different origin and pathogenicity. Arch. Virol. 146 (2001) 27-40
    • (2001) Arch. Virol. , vol.146 , pp. 27-40
    • Ayllón, M.A.1    López, C.2    Navas-Castillo, J.3    Garnsey, S.M.4    Guerri, J.5    Flores, R.6    Moreno, P.7
  • 5
    • 1942453734 scopus 로고    scopus 로고
    • cis-Acting elements at opposite ends of the Citrus tristeza virus genome differ in initiation and termination of subgenomic RNAs
    • Ayllón M.A., Gowda S., Satyanarayana T., and Dawson W.O. cis-Acting elements at opposite ends of the Citrus tristeza virus genome differ in initiation and termination of subgenomic RNAs. Virology 322 (2004) 41-50
    • (2004) Virology , vol.322 , pp. 41-50
    • Ayllón, M.A.1    Gowda, S.2    Satyanarayana, T.3    Dawson, W.O.4
  • 6
    • 33645717474 scopus 로고    scopus 로고
    • Variations in two gene sequences of Citrus tristeza virus after host passage
    • Ayllón M.A., Rubio L., Sentandreu V., Moya A., Guerri J., and Moreno P. Variations in two gene sequences of Citrus tristeza virus after host passage. Virus Genes 32 (2006) 119-128
    • (2006) Virus Genes , vol.32 , pp. 119-128
    • Ayllón, M.A.1    Rubio, L.2    Sentandreu, V.3    Moya, A.4    Guerri, J.5    Moreno, P.6
  • 8
    • 0002309273 scopus 로고
    • The epidemiology and control of Citrus tristeza disease
    • Plumb R.T., and Thresh J.M. (Eds), Blackwell Scientific Publications, Oxford, UK
    • Bar-Joseph M., Roistacher C.N., and Garnsey S.M. The epidemiology and control of Citrus tristeza disease. In: Plumb R.T., and Thresh J.M. (Eds). Plant Virus Epidemiology (1983), Blackwell Scientific Publications, Oxford, UK 61-72
    • (1983) Plant Virus Epidemiology , pp. 61-72
    • Bar-Joseph, M.1    Roistacher, C.N.2    Garnsey, S.M.3
  • 9
    • 0036176474 scopus 로고    scopus 로고
    • The detection of Tomato spotted wilt virus (TSWV) in individual thrips vectors using real time fluorescent RT-PCR (TaqMan)
    • Boonham N., Smith P., Walsh K., Tame J., Morris J., Spence N., Benison J., and Barker I. The detection of Tomato spotted wilt virus (TSWV) in individual thrips vectors using real time fluorescent RT-PCR (TaqMan). J. Virol. Methods 101 (2002) 37-48
    • (2002) J. Virol. Methods , vol.101 , pp. 37-48
    • Boonham, N.1    Smith, P.2    Walsh, K.3    Tame, J.4    Morris, J.5    Spence, N.6    Benison, J.7    Barker, I.8
  • 12
    • 0033775615 scopus 로고    scopus 로고
    • Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
    • Bustin S.A. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J. Mol. Endocrinol. 25 (2000) 169-193
    • (2000) J. Mol. Endocrinol. , vol.25 , pp. 169-193
    • Bustin, S.A.1
  • 13
    • 0036668567 scopus 로고    scopus 로고
    • Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems
    • Bustin S.A. Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. J. Mol. Endocrinol. 29 (2002) 23-39
    • (2002) J. Mol. Endocrinol. , vol.29 , pp. 23-39
    • Bustin, S.A.1
  • 14
    • 0003111832 scopus 로고
    • Comparison of different immunosorbent assays for Citrus tristeza virus (CTV) using CTV-specific monoclonal and polyclonal antibodies
    • Brlansky R.H., Lee R.F., and Timmer L.W. (Eds). IOCV, Riverside, CA
    • Cambra M., Camarasa E., Gorris M.T., Garnsey S.M., and Carbonell E. Comparison of different immunosorbent assays for Citrus tristeza virus (CTV) using CTV-specific monoclonal and polyclonal antibodies. In: Brlansky R.H., Lee R.F., and Timmer L.W. (Eds). Proceedings of the11th Conference of the International Organization of Citrus Virologists. IOCV, Riverside, CA (1991) 38-45
    • (1991) Proceedings of the11th Conference of the International Organization of Citrus Virologists , pp. 38-45
    • Cambra, M.1    Camarasa, E.2    Gorris, M.T.3    Garnsey, S.M.4    Carbonell, E.5
  • 17
    • 0036308577 scopus 로고    scopus 로고
    • A novel class of large and infectious defective RNAs of Citrus tristeza virus
    • Che X., Mawassi M., and Bar-Joseph M. A novel class of large and infectious defective RNAs of Citrus tristeza virus. Virology 298 (2002) 133-145
    • (2002) Virology , vol.298 , pp. 133-145
    • Che, X.1    Mawassi, M.2    Bar-Joseph, M.3
  • 18
    • 0037664351 scopus 로고    scopus 로고
    • Defective RNAs of Citrus tristeza virus analogous to Crinivirus genomic RNAs
    • Che X., Dawson W.O., and Bar-Joseph M. Defective RNAs of Citrus tristeza virus analogous to Crinivirus genomic RNAs. Virology 310 (2003) 298-309
    • (2003) Virology , vol.310 , pp. 298-309
    • Che, X.1    Dawson, W.O.2    Bar-Joseph, M.3
  • 19
    • 0002130413 scopus 로고
    • Mutation rates and rapid evolution of RNA viruses
    • Morse S.S. (Ed), Raven Press, New York
    • Domingo E., and Holland J.J. Mutation rates and rapid evolution of RNA viruses. In: Morse S.S. (Ed). Evolutionary Biology of Viruses (1994), Raven Press, New York 171-180
    • (1994) Evolutionary Biology of Viruses , pp. 171-180
    • Domingo, E.1    Holland, J.J.2
  • 20
    • 0037566471 scopus 로고    scopus 로고
    • Improvement of Barley yellow dwarf virus-PAV detection in single aphids using a fluorescent real time RT-PCR
    • Fabre F., Kervarrec C., Mieuzet L., Riault A.V., and Jacquot E. Improvement of Barley yellow dwarf virus-PAV detection in single aphids using a fluorescent real time RT-PCR. J. Virol. Methods 110 (2003) 51-60
    • (2003) J. Virol. Methods , vol.110 , pp. 51-60
    • Fabre, F.1    Kervarrec, C.2    Mieuzet, L.3    Riault, A.V.4    Jacquot, E.5
  • 22
    • 0001522220 scopus 로고
    • Seedling yellows, an unreported virus disease of citrus
    • Fraser L. Seedling yellows, an unreported virus disease of citrus. Agr. Gaz. N.S. Wales 63 (1952) 125-131
    • (1952) Agr. Gaz. N.S. Wales , vol.63 , pp. 125-131
    • Fraser, L.1
  • 23
    • 0032894224 scopus 로고    scopus 로고
    • Quantitative RT-PCR: pitfalls and potential
    • Freeman W.M., Walker S.J., and Vrana K.E. Quantitative RT-PCR: pitfalls and potential. Biotechniques 26 (1999) 112-125
    • (1999) Biotechniques , vol.26 , pp. 112-125
    • Freeman, W.M.1    Walker, S.J.2    Vrana, K.E.3
  • 25
    • 0035919640 scopus 로고    scopus 로고
    • Characterization of the cis-acting elements controlling subgenomic mRNAs of Citrus tristeza virus: production of positive- and negative-stranded 3′-terminal and positive-stranded 5′-terminal RNAs
    • Gowda S., Satyanarayana T., Ayllón M.A., Albiach-Martí M.R., Mawassi M., Rabindran S., Garnsey S.M., and Dawson W.O. Characterization of the cis-acting elements controlling subgenomic mRNAs of Citrus tristeza virus: production of positive- and negative-stranded 3′-terminal and positive-stranded 5′-terminal RNAs. Virology 286 (2001) 134-151
    • (2001) Virology , vol.286 , pp. 134-151
    • Gowda, S.1    Satyanarayana, T.2    Ayllón, M.A.3    Albiach-Martí, M.R.4    Mawassi, M.5    Rabindran, S.6    Garnsey, S.M.7    Dawson, W.O.8
  • 26
    • 0037213279 scopus 로고    scopus 로고
    • Transcription strategy in a Closterovirus: a novel 5′-proximal controller element of Citrus tristeza virus produces 5′ and 3′-terminal subgenomic RNAs and differs from 3′open reading frame controller elements
    • Gowda S., Ayllón M.A., Satyanarayana T., Bar-Joseph M., and Dawson W.O. Transcription strategy in a Closterovirus: a novel 5′-proximal controller element of Citrus tristeza virus produces 5′ and 3′-terminal subgenomic RNAs and differs from 3′open reading frame controller elements. J. Virol. 77 (2003) 340-352
    • (2003) J. Virol. , vol.77 , pp. 340-352
    • Gowda, S.1    Ayllón, M.A.2    Satyanarayana, T.3    Bar-Joseph, M.4    Dawson, W.O.5
  • 27
    • 0002433812 scopus 로고
    • Variability among Spanish Citrus tristeza virus isolates revealed by double-stranded-RNA analysis
    • Guerri J., Moreno P., Muñoz N., and Martínez M.E. Variability among Spanish Citrus tristeza virus isolates revealed by double-stranded-RNA analysis. Plant Pathol. 40 (1991) 38-44
    • (1991) Plant Pathol. , vol.40 , pp. 38-44
    • Guerri, J.1    Moreno, P.2    Muñoz, N.3    Martínez, M.E.4
  • 28
    • 0346655258 scopus 로고    scopus 로고
    • Comparison of RiboGreen and 18S rRNA quantitation for normalizing real-time RT-PCR expression analysis
    • Hashimoto J.G., Beadles-Bohling A.S., and Wiren K.M. Comparison of RiboGreen and 18S rRNA quantitation for normalizing real-time RT-PCR expression analysis. Biotechniques 36 (2004) 54-59
    • (2004) Biotechniques , vol.36 , pp. 54-59
    • Hashimoto, J.G.1    Beadles-Bohling, A.S.2    Wiren, K.M.3
  • 31
    • 22744455834 scopus 로고    scopus 로고
    • Genetic marker analysis of a global collection of isolates of Citrus tristeza virus: characterization and distribution of CTV genotypes and association with symptoms
    • Hilf M.E., Mavrodieva V.A., and Garnsey S.M. Genetic marker analysis of a global collection of isolates of Citrus tristeza virus: characterization and distribution of CTV genotypes and association with symptoms. Phytopathology 95 (2005) 909-917
    • (2005) Phytopathology , vol.95 , pp. 909-917
    • Hilf, M.E.1    Mavrodieva, V.A.2    Garnsey, S.M.3
  • 33
    • 0037118218 scopus 로고    scopus 로고
    • Improved detection of Sugarcane yellow leaf virus using a real-time fluorescent (TaqMan) RT-PCR assay
    • Korimbocus J., Coates D., Barker I., and Boonham N. Improved detection of Sugarcane yellow leaf virus using a real-time fluorescent (TaqMan) RT-PCR assay. J. Virol. Methods 103 (2002) 109-120
    • (2002) J. Virol. Methods , vol.103 , pp. 109-120
    • Korimbocus, J.1    Coates, D.2    Barker, I.3    Boonham, N.4
  • 34
    • 25444475785 scopus 로고    scopus 로고
    • A standard curve based method for relative real time PCR data processing
    • Larionov A., Krause A., and Miller W. A standard curve based method for relative real time PCR data processing. BMC Bioinformatics 6 (2005) 62
    • (2005) BMC Bioinformatics , vol.6 , pp. 62
    • Larionov, A.1    Krause, A.2    Miller, W.3
  • 37
    • 84986976552 scopus 로고
    • Separation and interference of strains from a Citrus tristeza virus isolate evidenced by biological activity and double-stranded RNA (dsRNA) analysis
    • Moreno P., Guerri J., Ballester-Olmos J.F., Albiach R., and Martínez M.E. Separation and interference of strains from a Citrus tristeza virus isolate evidenced by biological activity and double-stranded RNA (dsRNA) analysis. Plant Pathol. 42 (1993) 35-41
    • (1993) Plant Pathol. , vol.42 , pp. 35-41
    • Moreno, P.1    Guerri, J.2    Ballester-Olmos, J.F.3    Albiach, R.4    Martínez, M.E.5
  • 39
    • 0034014532 scopus 로고    scopus 로고
    • Detection of Potato mop top virus and Tobacco rattle virus using a multiplex real-time fluorescent reverse-transcription polymerase chain reaction assay
    • Mumford R., Walsh K., Barker I., and Boonham N. Detection of Potato mop top virus and Tobacco rattle virus using a multiplex real-time fluorescent reverse-transcription polymerase chain reaction assay. Phytopathology 90 (2000) 448-453
    • (2000) Phytopathology , vol.90 , pp. 448-453
    • Mumford, R.1    Walsh, K.2    Barker, I.3    Boonham, N.4
  • 40
    • 0342546481 scopus 로고    scopus 로고
    • A new procedure to differentiate Citrus tristeza virus isolates by hybridisation with digoxigenin-labelled cDNA probes
    • Narváez G., Skander B.S., Ayllón M.A., Rubio L., Guerri J., and Moreno P. A new procedure to differentiate Citrus tristeza virus isolates by hybridisation with digoxigenin-labelled cDNA probes. J. Virol. Methods 85 (2000) 83-92
    • (2000) J. Virol. Methods , vol.85 , pp. 83-92
    • Narváez, G.1    Skander, B.S.2    Ayllón, M.A.3    Rubio, L.4    Guerri, J.5    Moreno, P.6
  • 42
    • 0027420976 scopus 로고
    • A method combining immunocapture and PCR amplification in a microtiter plate for the detection of plant viruses and subviral pathogens
    • Nolasco G., de Blas C., Torres V., and Ponz F. A method combining immunocapture and PCR amplification in a microtiter plate for the detection of plant viruses and subviral pathogens. J. Virol. Methods 45 (1993) 201-218
    • (1993) J. Virol. Methods , vol.45 , pp. 201-218
    • Nolasco, G.1    de Blas, C.2    Torres, V.3    Ponz, F.4
  • 43
    • 0033559111 scopus 로고    scopus 로고
    • New device and method for capture, reverse transcription and nested PCR in a single closed-tube
    • Olmos A., Cambra M., Esteban O., Gorris M.T., and Terrada E. New device and method for capture, reverse transcription and nested PCR in a single closed-tube. Nucleic Acids Res. 27 (1999) 1564-1565
    • (1999) Nucleic Acids Res. , vol.27 , pp. 1564-1565
    • Olmos, A.1    Cambra, M.2    Esteban, O.3    Gorris, M.T.4    Terrada, E.5
  • 44
    • 13244257766 scopus 로고    scopus 로고
    • SYBR green-based real-time quantitative PCR assay for detection of West Nile virus circumvents false-negative results due to strain variability
    • Papin J.F., Vahrson W., and Dittmer D.P. SYBR green-based real-time quantitative PCR assay for detection of West Nile virus circumvents false-negative results due to strain variability. J. Clin. Microbiol. 42 (2004) 1511-1518
    • (2004) J. Clin. Microbiol. , vol.42 , pp. 1511-1518
    • Papin, J.F.1    Vahrson, W.2    Dittmer, D.P.3
  • 45
    • 0001338515 scopus 로고
    • A monoclonal antibody which discriminates strains of Citrus tristeza virus
    • Permar T.A., Garnsey S.M., Gumpf D.J., and Lee R.F. A monoclonal antibody which discriminates strains of Citrus tristeza virus. Phytopathology 80 (1990) 224-228
    • (1990) Phytopathology , vol.80 , pp. 224-228
    • Permar, T.A.1    Garnsey, S.M.2    Gumpf, D.J.3    Lee, R.F.4
  • 46
    • 0002748647 scopus 로고    scopus 로고
    • Development and validation of an externally standardised quantitative insulin like growth factor-1 (IGF-1) RT-PCR using Light-Cycler SYBR Green 1 technology
    • Meuer S., Wittwer C., and Nakagawara K.I. (Eds), Springer, Heidelberg, Germany
    • Pfaffl M. Development and validation of an externally standardised quantitative insulin like growth factor-1 (IGF-1) RT-PCR using Light-Cycler SYBR Green 1 technology. In: Meuer S., Wittwer C., and Nakagawara K.I. (Eds). Rapid Cycle Real-time PCR: Methods and Applications (2001), Springer, Heidelberg, Germany 281-291
    • (2001) Rapid Cycle Real-time PCR: Methods and Applications , pp. 281-291
    • Pfaffl, M.1
  • 47
    • 22144495696 scopus 로고    scopus 로고
    • Quantitative detection of Cucumber vein yellowing virus in susceptible and partially resistant plants using real-time PCR
    • Picó B., Sifres A., and Nuez F. Quantitative detection of Cucumber vein yellowing virus in susceptible and partially resistant plants using real-time PCR. J. Virol. Methods 128 (2005) 14-20
    • (2005) J. Virol. Methods , vol.128 , pp. 14-20
    • Picó, B.1    Sifres, A.2    Nuez, F.3
  • 48
    • 0034838268 scopus 로고    scopus 로고
    • LightCycler multiplex PCR for the laboratory diagnosis of common viral infections of the central nervous system
    • Read S.J., Mitchell J.J., and Fink C.G. LightCycler multiplex PCR for the laboratory diagnosis of common viral infections of the central nervous system. J. Clin. Microbiol. 39 (2001) 3056-3059
    • (2001) J. Clin. Microbiol. , vol.39 , pp. 3056-3059
    • Read, S.J.1    Mitchell, J.J.2    Fink, C.G.3
  • 49
    • 0031568919 scopus 로고    scopus 로고
    • Product differentiation by analysis of DNA melting curves during the polymerase chain reaction
    • Ririe K.M., Rasmussen R.P., and Witter C.T. Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal. Biochem. 245 (1997) 154-160
    • (1997) Anal. Biochem. , vol.245 , pp. 154-160
    • Ririe, K.M.1    Rasmussen, R.P.2    Witter, C.T.3
  • 51
    • 0028888302 scopus 로고
    • Citrus tristeza virus and its aphid vector Toxoptera citricida: threats to citrus production in the Caribbean and Central and North America
    • Rocha-Peña M.A., Lee R.F., Lastra R., Niblett C.L., Ochoa-Corona F.M., Garnsey S.M., and Yokomi R.K. Citrus tristeza virus and its aphid vector Toxoptera citricida: threats to citrus production in the Caribbean and Central and North America. Plant Dis. 79 (1995) 437-443
    • (1995) Plant Dis. , vol.79 , pp. 437-443
    • Rocha-Peña, M.A.1    Lee, R.F.2    Lastra, R.3    Niblett, C.L.4    Ochoa-Corona, F.M.5    Garnsey, S.M.6    Yokomi, R.K.7
  • 53
    • 0000779933 scopus 로고
    • Differential hybridization with cloned cDNA sequences for detecting a specific isolate of Citrus tristeza virus
    • Rosner A., Lee R.F., and Bar-Joseph M. Differential hybridization with cloned cDNA sequences for detecting a specific isolate of Citrus tristeza virus. Phytopathology 76 (1986) 820-824
    • (1986) Phytopathology , vol.76 , pp. 820-824
    • Rosner, A.1    Lee, R.F.2    Bar-Joseph, M.3
  • 54
    • 0034864956 scopus 로고    scopus 로고
    • Genetic variation of Citrus Tristeza Virus isolates from California and Spain: evidence for mixed infections and recombination
    • Rubio L., Ayllón M.A., Kong P., Fernández A., Polek M., Guerri J., Moreno P., and Falk B. Genetic variation of Citrus Tristeza Virus isolates from California and Spain: evidence for mixed infections and recombination. J. Virol. 75 (2001) 8054-8062
    • (2001) J. Virol. , vol.75 , pp. 8054-8062
    • Rubio, L.1    Ayllón, M.A.2    Kong, P.3    Fernández, A.4    Polek, M.5    Guerri, J.6    Moreno, P.7    Falk, B.8
  • 55
    • 31144456370 scopus 로고    scopus 로고
    • The complete nucleotide sequence of a severe stem pitting isolate of Citrus tristeza virus from Spain: comparison with isolates from different origins
    • Ruiz-Ruiz S., Moreno P., Guerri J., and Ambrós S. The complete nucleotide sequence of a severe stem pitting isolate of Citrus tristeza virus from Spain: comparison with isolates from different origins. Arch. Virol. 151 (2006) 387-398
    • (2006) Arch. Virol. , vol.151 , pp. 387-398
    • Ruiz-Ruiz, S.1    Moreno, P.2    Guerri, J.3    Ambrós, S.4
  • 56
    • 4444337502 scopus 로고    scopus 로고
    • Mathematics of quantitative kinetic PCR and the application of standard curves
    • Rutledge R.G., and Coté C. Mathematics of quantitative kinetic PCR and the application of standard curves. Nucleic Acids Res. 31 (2003) e93
    • (2003) Nucleic Acids Res. , vol.31
    • Rutledge, R.G.1    Coté, C.2
  • 57
    • 33645708840 scopus 로고    scopus 로고
    • Polymorphism of a specific region in the gene p23 of Citrus tristeza virus allows differentiation between mild and severe isolates
    • Sambade A., López C., Rubio L., Flores R., Guerri J., and Moreno P. Polymorphism of a specific region in the gene p23 of Citrus tristeza virus allows differentiation between mild and severe isolates. Arch. Virol. 148 (2003) 2281-2291
    • (2003) Arch. Virol. , vol.148 , pp. 2281-2291
    • Sambade, A.1    López, C.2    Rubio, L.3    Flores, R.4    Guerri, J.5    Moreno, P.6
  • 61
    • 1542726119 scopus 로고    scopus 로고
    • Diagnostic value of real-time capillary thermal cycler in virus detection
    • Tan B.H., Lim E.A., Liaw J.C., Seah S.G., and Yap E.P. Diagnostic value of real-time capillary thermal cycler in virus detection. Expert Rev. Mol. Diagn. 4 (2004) 219-230
    • (2004) Expert Rev. Mol. Diagn. , vol.4 , pp. 219-230
    • Tan, B.H.1    Lim, E.A.2    Liaw, J.C.3    Seah, S.G.4    Yap, E.P.5
  • 62
    • 11144315425 scopus 로고    scopus 로고
    • Detection and differentiation of Plum pox virus using real-time multiplex PCR with SYBR Green and melting curve analysis: a rapid method for strain typing
    • Varga A., and James D. Detection and differentiation of Plum pox virus using real-time multiplex PCR with SYBR Green and melting curve analysis: a rapid method for strain typing. J. Virol. Methods 123 (2005) 213-220
    • (2005) J. Virol. Methods , vol.123 , pp. 213-220
    • Varga, A.1    James, D.2
  • 63
    • 0022631591 scopus 로고
    • Production and characterization of monoclonal antibodies specific for Citrus tristeza virus and their use for diagnosis
    • Vela C., Cambra M., Cortés E., Moreno P., Miguet J.G., Pérez de San Román C., and Sanz A. Production and characterization of monoclonal antibodies specific for Citrus tristeza virus and their use for diagnosis. J. Gen. Virol. 67 (1986) 91-96
    • (1986) J. Gen. Virol. , vol.67 , pp. 91-96
    • Vela, C.1    Cambra, M.2    Cortés, E.3    Moreno, P.4    Miguet, J.G.5    Pérez de San Román, C.6    Sanz, A.7
  • 65
    • 11344264156 scopus 로고    scopus 로고
    • Evidence of multiple recombination events between two RNA sequence variants within a Citrus tristeza virus isolate
    • Vives M.C., Rubio L., Sambade A., Mirkov T.E., Moreno P., and Guerri J. Evidence of multiple recombination events between two RNA sequence variants within a Citrus tristeza virus isolate. Virology 331 (2005) 232-237
    • (2005) Virology , vol.331 , pp. 232-237
    • Vives, M.C.1    Rubio, L.2    Sambade, A.3    Mirkov, T.E.4    Moreno, P.5    Guerri, J.6
  • 66
    • 0142071135 scopus 로고    scopus 로고
    • A method for the absolute quantification of cDNA using real-time PCR
    • Whelan J.A., Rusell N.B., and Whelan M.A. A method for the absolute quantification of cDNA using real-time PCR. J. Immunol. Methods 278 (2003) 261-269
    • (2003) J. Immunol. Methods , vol.278 , pp. 261-269
    • Whelan, J.A.1    Rusell, N.B.2    Whelan, M.A.3
  • 67
    • 0030731687 scopus 로고    scopus 로고
    • Involvement of a subgenomic mRNA in the generation of a variable population of defective Citrus tristeza virus molecules
    • Yang G., Mawassi M., Gofman R., Gafny R., and Bar-Joseph M. Involvement of a subgenomic mRNA in the generation of a variable population of defective Citrus tristeza virus molecules. J. Virol. 71 (1997) 9800-9802
    • (1997) J. Virol. , vol.71 , pp. 9800-9802
    • Yang, G.1    Mawassi, M.2    Gofman, R.3    Gafny, R.4    Bar-Joseph, M.5
  • 68
    • 0028052540 scopus 로고
    • Establishment of the brown citrus aphid (Homoptera: Aphididae) in Central America and the Caribbean basin and transmission of Citrus tristeza virus
    • Yokomi R.K., Lastra R., Stoetzel M.B., Lee R.F., Garnsey S.M., Gottwald T.R.M., Rocha-Peña M.A., and Niblett C.L. Establishment of the brown citrus aphid (Homoptera: Aphididae) in Central America and the Caribbean basin and transmission of Citrus tristeza virus. J. Econ. Entomol. 87 (1994) 1078-1085
    • (1994) J. Econ. Entomol. , vol.87 , pp. 1078-1085
    • Yokomi, R.K.1    Lastra, R.2    Stoetzel, M.B.3    Lee, R.F.4    Garnsey, S.M.5    Gottwald, T.R.M.6    Rocha-Peña, M.A.7    Niblett, C.L.8


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.