Rapid discrimination of Tomato chlorosis virus, Tomato infectious chlorosis virus and co-amplification of plant internal control using real-time RT-PCR

Journal of Virological Methods

Volumn 176, Issue 1-2, 2011, Pages 53-59

  Papayiannis, Lambros C ^a   Harkou, Ivi S ^a   Markou, Yiannis M ^a   Demetriou, Christos N ^b   Katis, Nikolaos I ^b  

^a AGRICULTURAL RESEARCH INSTITUTE   (Cyprus)

^b ARISTOTLE UNIVERSITY OF THESSALONIKI   (Greece)

Author keywords

Crinivirus; Hydrolysis probes; Nylon membrane; Plant internal control; Real time RT PCR; TICV; ToCV

Indexed keywords

NYLON; PLANT EXTRACT; SAP EXTRACT; UNCLASSIFIED DRUG;

ARTICLE; ARTIFICIAL MEMBRANE; ASSAY; CHLOROSIS; CONTROLLED STUDY; CRINIVIRUS; CYTOCHROME OXIDASE SUBUNIT 1 GENE; DISEASE CARRIER; ELUTION; FREEZING; GENE AMPLIFICATION; GEOGRAPHIC ORIGIN; MITOCHONDRIAL GENE; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; ROOM TEMPERATURE; SENSITIVITY AND SPECIFICITY; STORAGE TEMPERATURE; SWEET POTATO WHITEFLY; TOMATO CHLOROSIS VIRUS; TOMATO INFECTIOUS CHLOROSIS VIRUS; TRIALEURODES VAPORARIORUM; VIRUS DETECTION; WHITEFLY;

ANIMALS; CRINIVIRUS; ELECTRON TRANSPORT COMPLEX IV; HEMIPTERA; INSECT VECTORS; LYCOPERSICON ESCULENTUM; MEMBRANES, ARTIFICIAL; MITOCHONDRIA; NYLONS; PLANT DISEASES; PLANT PROTEINS; REFERENCE STANDARDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; TAQ POLYMERASE; TIME FACTORS;

ALEYRODIDAE; CLOSTEROVIRIDAE; CRINIVIRUS; LYCOPERSICON ESCULENTUM; TOMATO CHLOROSIS VIRUS; TOMATO INFECTIOUS CHLOROSIS VIRUS;

EID: 79960578341     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2011.05.036     Document Type: Article

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