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Volumn 401, Issue 1, 2011, Pages 3-13

Quantitative single-cell gene expression measurements of multiple genes in response to hypoxia treatment

Author keywords

Gene expression; Hypoxia treatment; Single cell

Indexed keywords

BIOLOGICAL SIGNIFICANCE; CELL POPULATIONS; CELL PROCESS; EXPERIMENTAL TECHNIQUES; EXPRESSION LEVELS; EXPRESSION MEASUREMENTS; FLUORESCENT DYES; FURTHER DEVELOPMENT; GENE EXPRESSION LEVELS; GENE EXPRESSION PROFILING; GENE TRANSCRIPTIONS; HYPOXIA TREATMENT; MAMMALIAN CELLS; METHOD DEVELOPMENT; MULTIPLE GENES; PHYSIOLOGICAL CONDITION; POINT OF CARE; SINGLE CELLS; SINGLE-CELL; SINGLE-CELL LEVEL; SYBR GREEN; TECHNICAL DETAILS; TWO-STEP PROCEDURE; WIDE DYNAMIC RANGE;

EID: 79960555292     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-011-5084-2     Document Type: Article
Times cited : (11)

References (49)
  • 1
    • 67449141030 scopus 로고    scopus 로고
    • Chaotic expression dynamics implies pluripotency: When theory and experiment meet
    • 10.1186/1745-6150-4-17
    • C Furusawa K Kaneko 2009 Chaotic expression dynamics implies pluripotency: when theory and experiment meet Biol Direct 4 17 28 10.1186/1745-6150-4-17
    • (2009) Biol Direct , vol.4 , pp. 17-28
    • Furusawa, C.1    Kaneko, K.2
  • 2
    • 41749107942 scopus 로고    scopus 로고
    • Stochasticity and cell fate
    • DOI 10.1126/science.1147888
    • R Losick C Desplan 2008 Stochasticity and cell fate Science 320 65 68 10.1126/science.1147888 1:CAS:528:DC%2BD1cXktVKhtL0%3D (Pubitemid 351490752)
    • (2008) Science , vol.320 , Issue.5872 , pp. 65-68
    • Losick, R.1    Desplan, C.2
  • 3
    • 0037008488 scopus 로고    scopus 로고
    • Single-cell gene expression profiling
    • DOI 10.1126/science.1072241
    • JM Levsky SM Shenoy RC Pezo RH Singer 2002 Single-cell gene expression profiling Science 297 836 840 10.1126/science.1072241 1:CAS:528: DC%2BD38XlvV2jsLY%3D (Pubitemid 34845918)
    • (2002) Science , vol.297 , Issue.5582 , pp. 836-840
    • Levsky, J.M.1    Shenoy, S.M.2    Pezo, R.C.3    Singer, R.H.4
  • 5
    • 62449123034 scopus 로고    scopus 로고
    • A chance at survival: Gene expression noise and phenotypic diversification strategies
    • 10.1111/j.1365-2958.2009.06605.x 1:CAS:528:DC%2BD1MXktVWhs7w%3D
    • D Fraser M Kaern 2009 A chance at survival: gene expression noise and phenotypic diversification strategies Mol Microbiol 71 1333 1340 10.1111/j.1365-2958.2009.06605.x 1:CAS:528:DC%2BD1MXktVWhs7w%3D
    • (2009) Mol Microbiol , vol.71 , pp. 1333-1340
    • Fraser, D.1    Kaern, M.2
  • 6
    • 0037119587 scopus 로고    scopus 로고
    • Stochastic gene expression in a single cell
    • DOI 10.1126/science.1070919
    • MB Elowitz AJ Levine ED Siggia PS Swain 2002 Stochastic gene expression in a single cell Science 297 1183 1186 10.1126/science.1070919 1:CAS:528:DC%2BD38XmsVOht7c%3D (Pubitemid 36193353)
    • (2002) Science , vol.297 , Issue.5584 , pp. 1183-1186
    • Elowitz, M.B.1    Levine, A.J.2    Siggia, E.D.3    Swain, P.S.4
  • 7
    • 68149170798 scopus 로고    scopus 로고
    • Resolving cell population heterogeneity: Real-time PCR for simultaneous multiplexed gene detection in multiple single-cell samples
    • 10.1371/journal.pone.0006326
    • A Diercks H Kostner A Ozinsky 2009 Resolving cell population heterogeneity: real-time PCR for simultaneous multiplexed gene detection in multiple single-cell samples PLoS ONE 4 e6326 10.1371/journal.pone.0006326
    • (2009) PLoS ONE , vol.4 , pp. 6326
    • Diercks, A.1    Kostner, H.2    Ozinsky, A.3
  • 8
    • 67649586670 scopus 로고    scopus 로고
    • Quantitative analysis of gene expression in a single cell by qPCR
    • 10.1038/nmeth.1338 1:CAS:528:DC%2BD1MXnt1Sht7Y%3D
    • K Taniguchi T Kajiyama H Kambara 2009 Quantitative analysis of gene expression in a single cell by qPCR Nat Methods 6 503 506 10.1038/nmeth.1338 1:CAS:528:DC%2BD1MXnt1Sht7Y%3D
    • (2009) Nat Methods , vol.6 , pp. 503-506
    • Taniguchi, K.1    Kajiyama, T.2    Kambara, H.3
  • 9
    • 48249156189 scopus 로고    scopus 로고
    • Quantification of mRNA in single cells and modelling of RT-qPCR induced noise
    • 10.1186/1471-2199-9-63
    • M Bengtsson M Hemberg P Rorsman A Stahlberg 2008 Quantification of mRNA in single cells and modelling of RT-qPCR induced noise BMC Mol Biol 9 63 74 10.1186/1471-2199-9-63
    • (2008) BMC Mol Biol , vol.9 , pp. 63-74
    • Bengtsson, M.1    Hemberg, M.2    Rorsman, P.3    Stahlberg, A.4
  • 10
    • 77649238068 scopus 로고    scopus 로고
    • Single-cell gene expression profiling using reverse transcription quantitative real-time PCR
    • 10.1016/j.ymeth.2010.01.002 1:CAS:528:DC%2BC3cXjtVCgu7k%3D
    • A Stahlberg M Bengtsson 2010 Single-cell gene expression profiling using reverse transcription quantitative real-time PCR Methods 50 282 288 10.1016/j.ymeth.2010.01.002 1:CAS:528:DC%2BC3cXjtVCgu7k%3D
    • (2010) Methods , vol.50 , pp. 282-288
    • Stahlberg, A.1    Bengtsson, M.2
  • 11
    • 13244265562 scopus 로고    scopus 로고
    • Rapid, single-tube method for quantitative preparation and analysis of RNA and DNA in samples as small as one cell
    • DOI 10.1186/1472-6750-5-2
    • C Hartshorn A Anshelevich LJ Wangh 2005 Rapid, single-tube method for quantitative preparation and analysis of RNA and DNA in samples as small as one cell BMC Biotechnol 5 2 15 10.1186/1472-6750-5-2 (Pubitemid 40195479)
    • (2005) BMC Biotechnology , vol.5 , pp. 2
    • Hartshorn, C.1    Anshelevich, A.2    Wangh, L.J.3
  • 12
    • 39549088479 scopus 로고    scopus 로고
    • Single-cell duplex RT-LATE-PCR reveals Oct4 and Xist RNA gradients in 8-cell embryos
    • DOI 10.1186/1472-6750-7-87
    • C Hartshorn JJ Eckert O Hartung LJ Wangh 2007 Single-cell duplex RT-LATE-PCR reveals Oct4 and Xist RNA gradients in 8-cell embryos BMC Biotechnol 7 87 101 10.1186/1472-6750-7-87 (Pubitemid 351280040)
    • (2007) BMC Biotechnology , vol.7 , pp. 87
    • Hartshorn, C.1    Eckert, J.J.2    Hartung, O.3    Wangh, L.J.4
  • 13
    • 77956116622 scopus 로고    scopus 로고
    • Massively parallel detection of gene expression in single cells using subnanolitre wells
    • 10.1039/c004847j 1:CAS:528:DC%2BC3cXhtVKrs7jF
    • YA Gong AO Ogunniyi JC Love 2010 Massively parallel detection of gene expression in single cells using subnanolitre wells Lab Chip 10 2334 2337 10.1039/c004847j 1:CAS:528:DC%2BC3cXhtVKrs7jF
    • (2010) Lab Chip , vol.10 , pp. 2334-2337
    • Gong, Y.A.1    Ogunniyi, A.O.2    Love, J.C.3
  • 14
    • 77956291361 scopus 로고    scopus 로고
    • Quantitative estimation of multiple miRNAs and mRNAs from a single cell
    • 2010 pdb prot5478
    • Joglekar MV, Wei C, Hardikar AA (2010) Quantitative estimation of multiple miRNAs and mRNAs from a single cell. Cold Spring Harb Protoc 2010: pdb prot5478
    • (2010) Cold Spring Harb Protoc
    • Joglekar, M.V.1    Wei, C.2    Hardikar, A.A.3
  • 15
    • 57149142495 scopus 로고    scopus 로고
    • The impact of O2 availability on human cancer
    • 10.1038/nrc2540 1:CAS:528:DC%2BD1cXhsVWhu7fO
    • JA Bertout SA Patel MC Simon 2008 The impact of O2 availability on human cancer Nat Rev Cancer 8 967 975 10.1038/nrc2540 1:CAS:528:DC%2BD1cXhsVWhu7fO
    • (2008) Nat Rev Cancer , vol.8 , pp. 967-975
    • Bertout, J.A.1    Patel, S.A.2    Simon, M.C.3
  • 16
    • 34247383169 scopus 로고    scopus 로고
    • Why do tumors metastasize?
    • M Lopez-Lazaro 2007 Why do tumors metastasize? Cancer Biol Ther 6 141 144 10.4161/cbt.6.2.3950 1:CAS:528:DC%2BD2sXhtVeqsb%2FL (Pubitemid 46641613)
    • (2007) Cancer Biology and Therapy , vol.6 , Issue.2 , pp. 141-144
    • Lopez-Lazaro, M.1
  • 17
    • 77951162650 scopus 로고    scopus 로고
    • Hypoxia-inducible factors in stem cells and cancer
    • 10.1111/j.1582-4934.2009.00963.x 1:CAS:528:DC%2BC3cXkvFGjs7w%3D
    • J Mazumdar V Dondeti MC Simon 2009 Hypoxia-inducible factors in stem cells and cancer J Cell Mol Med 13 4319 4328 10.1111/j.1582-4934.2009.00963.x 1:CAS:528:DC%2BC3cXkvFGjs7w%3D
    • (2009) J Cell Mol Med , vol.13 , pp. 4319-4328
    • Mazumdar, J.1    Dondeti, V.2    Simon, M.C.3
  • 18
    • 79955094707 scopus 로고    scopus 로고
    • Quantitative RT-qPCR analysis of gene expression in single bacterial cells
    • 10.1016/j.mimet.2011.03.008 1:CAS:528:DC%2BC3MXlt1Sgsbk%3D
    • W Gao W Zhang DR Meldrum 2011 Quantitative RT-qPCR analysis of gene expression in single bacterial cells J Microbiol Meth 85 221 227 10.1016/j.mimet.2011.03.008 1:CAS:528:DC%2BC3MXlt1Sgsbk%3D
    • (2011) J Microbiol Meth , vol.85 , pp. 221-227
    • Gao, W.1    Zhang, W.2    Meldrum, D.R.3
  • 20
    • 0029935566 scopus 로고    scopus 로고
    • Expression of vascular endothelial growth factor, its receptor, and other angiogenic factors in human breast cancer
    • H Yoshiji DE Gomez M Shibuya UP Thorgeirsson 1996 Expression of vascular endothelial growth factor, its receptor, and other angiogenic factors in human breast cancer Cancer Res 56 2013 2016 1:CAS:528:DyaK28Xis1ekt74%3D (Pubitemid 26119899)
    • (1996) Cancer Research , vol.56 , Issue.9 , pp. 2013-2016
    • Yoshiji, H.1    Gomez, D.E.2    Shibuya, M.3    Thorgeirsson, U.P.4
  • 21
    • 4243953217 scopus 로고    scopus 로고
    • A role for AMP-activated protein kinase in contraction and hypoxia regulated glucose transport in skeletal muscle
    • J Mu MJ Birnbaum 2001 A role for AMP-activated protein kinase in contraction and hypoxia regulated glucose transport in skeletal muscle FASEB J 15 A1164 A1164
    • (2001) FASEB J , vol.15
    • Mu, J.1    Birnbaum, M.J.2
  • 23
    • 39149112487 scopus 로고    scopus 로고
    • PCR arrays identify metallothionein-3 as a highly hypoxia-inducible gene in human adipocytes
    • 10.1016/j.bbrc.2008.01.036 1:CAS:528:DC%2BD1cXitVGgu7s%3D
    • B Wang IS Wood P Trayhurn 2008 PCR arrays identify metallothionein-3 as a highly hypoxia-inducible gene in human adipocytes Biochem Biophys Res Commun 368 88 93 10.1016/j.bbrc.2008.01.036 1:CAS:528:DC%2BD1cXitVGgu7s%3D
    • (2008) Biochem Biophys Res Commun , vol.368 , pp. 88-93
    • Wang, B.1    Wood, I.S.2    Trayhurn, P.3
  • 24
    • 58149215819 scopus 로고    scopus 로고
    • Hypoxia upregulates the expression of angiopoietin-like-4 in human articular chondrocytes: Role of angiopoietin-like-4 in the expression of matrix metalloproteinases and cartilage degradation
    • 10.1002/jor.20703 1:CAS:528:DC%2BD1MXhtFyhsbY%3D
    • M Murata K Yudo H Nakamura J Chiba K Okamoto N Suematsu K Nishioka M Beppu K Inoue T Kato K Masuko 2009 Hypoxia upregulates the expression of angiopoietin-like-4 in human articular chondrocytes: role of angiopoietin-like-4 in the expression of matrix metalloproteinases and cartilage degradation J Orthop Res 27 50 57 10.1002/jor.20703 1:CAS:528:DC%2BD1MXhtFyhsbY%3D
    • (2009) J Orthop Res , vol.27 , pp. 50-57
    • Murata, M.1    Yudo, K.2    Nakamura, H.3    Chiba, J.4    Okamoto, K.5    Suematsu, N.6    Nishioka, K.7    Beppu, M.8    Inoue, K.9    Kato, T.10    Masuko, K.11
  • 26
    • 0033575003 scopus 로고    scopus 로고
    • Direct comparison of GAPDH, β-actin, cyclophilin, and 28S rRNA as internal standards for quantifying RNA levels under hypoxia
    • DOI 10.1006/bbrc.1999.0815
    • H Zhong JW Simons 1999 Direct comparison of GAPDH, beta-actin, cyclophilin, and 28S rRNA as internal standards for quantifying RNA levels under hypoxia Biochem Biophys Res Commun 259 523 526 10.1006/bbrc.1999.0815 1:CAS:528:DyaK1MXjs1Gntbk%3D (Pubitemid 29335566)
    • (1999) Biochemical and Biophysical Research Communications , vol.259 , Issue.3 , pp. 523-526
    • Zhong, H.1    Simons, J.W.2
  • 27
    • 17044387386 scopus 로고    scopus 로고
    • Hsp70 chaperones: Cellular functions and molecular mechanism
    • 10.1007/s00018-004-4464-6 1:CAS:528:DC%2BD2MXktlymt78%3D
    • MP Mayer B Bukau 2005 Hsp70 chaperones: cellular functions and molecular mechanism Cell Mol Life Sci 62 670 684 10.1007/s00018-004-4464-6 1:CAS:528:DC%2BD2MXktlymt78%3D
    • (2005) Cell Mol Life Sci , vol.62 , pp. 670-684
    • Mayer, M.P.1    Bukau, B.2
  • 28
    • 0035947235 scopus 로고    scopus 로고
    • A role for AMP-activated protein kinase in contraction- and hypoxia-regulated glucose transport in skeletal muscle
    • DOI 10.1016/S1097-2765(01)00251-9
    • J Mu JT Brozinick O Valladares M Bucan MJ Birnbaum 2001 A role for AMP-activated protein kinase in contraction- and hypoxia-regulated glucose transport in skeletal muscle Mol Cell 7 1085 1094 10.1016/S1097-2765(01)00251-9 1:CAS:528:DC%2BD3MXktFehtrw%3D (Pubitemid 32525754)
    • (2001) Molecular Cell , vol.7 , Issue.5 , pp. 1085-1094
    • Mu, J.1    Brozinick Jr., J.T.2    Valladares, O.3    Bucan, M.4    Birnbaum, M.J.5
  • 29
    • 77949547666 scopus 로고    scopus 로고
    • A new cross-linkable oxygen sensor covalently bonded into poly(2-hydroxyethyl methacrylate)-co-polyacrylamide thin film for dissolved oxygen sensing
    • 10.1021/cm903361y 1:CAS:528:DC%2BC3cXhs1GgurY%3D
    • YQ Tian BR Shumway DR Meldrum 2010 A new cross-linkable oxygen sensor covalently bonded into poly(2-hydroxyethyl methacrylate)-co-polyacrylamide thin film for dissolved oxygen sensing Chem Mater 22 2069 2078 10.1021/cm903361y 1:CAS:528:DC%2BC3cXhs1GgurY%3D
    • (2010) Chem Mater , vol.22 , pp. 2069-2078
    • Tian, Y.Q.1    Shumway, B.R.2    Meldrum, D.R.3
  • 30
    • 77954383219 scopus 로고    scopus 로고
    • Automated selection and placement of single cells using vision-based feedback control
    • 10.1109/TASE.2009.2035709
    • YH Anis MR Holl DR Meldrum 2010 Automated selection and placement of single cells using vision-based feedback control IEEE Trans Autom Sci Eng 7 598 606 10.1109/TASE.2009.2035709
    • (2010) IEEE Trans Autom Sci Eng , vol.7 , pp. 598-606
    • Anis, Y.H.1    Holl, M.R.2    Meldrum, D.R.3
  • 31
    • 33644975004 scopus 로고    scopus 로고
    • RNA integrity and the effect on the real-time qRT-PCR performance
    • 10.1016/j.mam.2005.12.003 1:CAS:528:DC%2BD28XisF2gsrg%3D
    • S Fleige MW Pfaffl 2006 RNA integrity and the effect on the real-time qRT-PCR performance Mol Aspects Med 27 126 139 10.1016/j.mam.2005.12.003 1:CAS:528:DC%2BD28XisF2gsrg%3D
    • (2006) Mol Aspects Med , vol.27 , pp. 126-139
    • Fleige, S.1    Pfaffl, M.W.2
  • 32
    • 33751341522 scopus 로고    scopus 로고
    • Extracellular matrix-bound angiopoietin-like 4 inhibits endothelial cell adhesion, migration, and sprouting and alters actin cytoskeleton
    • DOI 10.1161/01.RES.0000250758.63358.91, PII 0000301220061124000010
    • A Cazes A Galaup C Chomel M Bignon N Bréchot S Le Jan H Weber P Corvol L Muller S Germain C Monnot 2006 Extracellular matrix-bound angiopoietin-like 4 inhibits endothelial cell adhesion, migration, and sprouting and alters actin cytoskeleton Circ Res 99 1207 1215 10.1161/01.RES.0000250758. 63358.91 1:CAS:528:DC%2BD28Xht1antr3I (Pubitemid 44808657)
    • (2006) Circulation Research , vol.99 , Issue.11 , pp. 1207-1215
    • Cazes, A.1    Galaup, A.2    Chomel, C.3    Bignon, M.4    Brechot, N.5    Jan, S.L.6    Weber, H.7    Corvol, P.8    Muller, L.9    Germain, S.10    Monnot, C.11
  • 37
    • 0029103274 scopus 로고
    • Hypoxia regulates vascular endothelial growth factor gene expression in endothelial cells identification of a 5' enhancer
    • 1:CAS:528:DyaK2MXns1altLs%3D
    • YX Liu SR Cox T Morita S Kourembanas 1995 Hypoxia regulates vascular endothelial growth factor gene expression in endothelial cells identification of a 5' enhancer Circ Res 77 638 643 1:CAS:528:DyaK2MXns1altLs%3D
    • (1995) Circ Res , vol.77 , pp. 638-643
    • Liu, Y.X.1    Cox, S.R.2    Morita, T.3    Kourembanas, S.4
  • 38
    • 70449481835 scopus 로고    scopus 로고
    • Validation of suitable house keeping genes for hypoxia-cultured human chondrocytes
    • 10.1186/1471-2199-10-94
    • CB Foldager S Munir M Ulrik-Vinther K Søballe C Bünger M Lind 2009 Validation of suitable house keeping genes for hypoxia-cultured human chondrocytes BMC Mol Biol 10 94 102 10.1186/1471-2199-10-94
    • (2009) BMC Mol Biol , vol.10 , pp. 94-102
    • Foldager, C.B.1    Munir, S.2    Ulrik-Vinther, M.3    Søballe, K.4    Bünger, C.5    Lind, M.6
  • 41
    • 28944434119 scopus 로고    scopus 로고
    • Real-time kinetics of gene activity in individual bacteria
    • DOI 10.1016/j.cell.2005.09.031, PII S0092867405010378
    • I Golding J Paulsson SM Zawilski EC Cox 2005 Real-time kinetics of gene activity in individual bacteria Cell 123 1025 1036 10.1016/j.cell.2005.09.031 1:CAS:528:DC%2BD28XptVOg (Pubitemid 41785418)
    • (2005) Cell , vol.123 , Issue.6 , pp. 1025-1036
    • Golding, I.1    Paulsson, J.2    Zawilski, S.M.3    Cox, E.C.4
  • 43
    • 34948874233 scopus 로고    scopus 로고
    • Cell-to-cell heterogeneity in growth rate and gene expression in Methylobacterium extorquens AM1
    • DOI 10.1128/JB.00746-07
    • TJ Strovas LM Sauter X Guo ME Lidstrom 2007 Cell-to-cell heterogeneity in growth rate and gene expression in Methylobacterium extorquens AM1 J Bacteriol 189 7127 7133 10.1128/JB.00746-07 1:CAS:528:DC%2BD2sXhtFKmsLvJ (Pubitemid 47525458)
    • (2007) Journal of Bacteriology , vol.189 , Issue.19 , pp. 7127-7133
    • Strovas, T.J.1    Sauter, L.M.2    Guo, X.3    Lidstrom, M.E.4
  • 44
    • 65249157060 scopus 로고    scopus 로고
    • Single-cell profiling reveals the origin of phenotypic variability in adipogenesis
    • 10.1371/journal.pone.0005189
    • TT Le JX Cheng 2009 Single-cell profiling reveals the origin of phenotypic variability in adipogenesis PLoS ONE 4 e5189 10.1371/journal.pone. 0005189
    • (2009) PLoS ONE , vol.4 , pp. 5189
    • Le, T.T.1    Cheng, J.X.2
  • 46
    • 77956892318 scopus 로고    scopus 로고
    • The role of physiological heterogeneity in microbial population behavior
    • 10.1038/nchembio.436 1:CAS:528:DC%2BC3cXhtFOht7fM
    • ME Lidstrom MC Konopka 2010 The role of physiological heterogeneity in microbial population behavior Nat Chem Biol 6 705 712 10.1038/nchembio.436 1:CAS:528:DC%2BC3cXhtFOht7fM
    • (2010) Nat Chem Biol , vol.6 , pp. 705-712
    • Lidstrom, M.E.1    Konopka, M.C.2
  • 47
    • 50349085576 scopus 로고    scopus 로고
    • Tightly regulated and heritable division control in single bacterial cells
    • 10.1529/biophysj.108.128785 1:CAS:528:DC%2BD1cXpslKksL0%3D
    • D Siegal-Gaskins S Crosson 2008 Tightly regulated and heritable division control in single bacterial cells Biophys J 95 2063 2072 10.1529/biophysj.108. 128785 1:CAS:528:DC%2BD1cXpslKksL0%3D
    • (2008) Biophys J , vol.95 , pp. 2063-2072
    • Siegal-Gaskins, D.1    Crosson, S.2
  • 48
    • 25844433114 scopus 로고    scopus 로고
    • Gene expression profiling in single cells from the pancreatic islets of Langerhans reveals lognormal distribution of mRNA levels
    • DOI 10.1101/gr.3820805
    • M Bengtsson A Stahlberg P Rorsman M Kubista 2005 Gene expression profiling in single cells from the pancreatic islets of Langerhans reveals lognormal distribution of mRNA levels Genome Res 15 1388 1392 10.1101/gr.3820805 1:CAS:528:DC%2BD2MXhtFajurnM (Pubitemid 41400905)
    • (2005) Genome Research , vol.15 , Issue.10 , pp. 1388-1392
    • Bengtsson, M.1    Stahlberg, A.2    Rorsman, P.3    Kubista, M.4
  • 49
    • 14744280811 scopus 로고    scopus 로고
    • Microfluidic systems for integrated, high-throughput DNA analysis
    • RT Kelly AT Woolley 2005 Microfluidic systems for integrated, high-throughput DNA analysis Anal Chem 77 96a 102a 1:CAS:528: DC%2BD2MXhsleht7Y%3D (Pubitemid 40327853)
    • (2005) Analytical Chemistry , vol.77 , Issue.5
    • Kelly, R.T.1    Woolley, A.T.2


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