High-content screening for chemical modulators of embryonal carcinoma cell differentiation and survival

Journal of Biomolecular Screening

Volumn 16, Issue 6, 2011, Pages 603-617

  Barbaric, Ivana ^a   Jones, Mark ^a   Harley, David J ^a   Gokhale, Paul J ^a   Andrews, Peter W ^a  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

embryonal carcinoma cell; embryonic stem cell; high content screening; kinase inhibitors

Indexed keywords

2 [1 (3 DIMETHYLAMINOPROPYL) 3 INDOLYL] 3 (3 INDOLYL)MALEIMIDE; 4 (1 AMINOETHYL) N (4 PYRIDYL)CYCLOHEXANECARBOXAMIDE; FASUDIL; N (2 METHYLAMINOETHYL) 5 ISOQUINOLINESULFONAMIDE; ROTTLERIN;

ARTICLE; CANCER CELL CULTURE; CELL COUNT; CELL DIFFERENTIATION; CELL SURVIVAL; CONTROLLED STUDY; DRUG MECHANISM; EMBRYONAL CARCINOMA STEM CELL; HUMAN; HUMAN CELL; PRIORITY JOURNAL;

ACETOPHENONES; BENZOPYRANS; CELL DIFFERENTIATION; CELL SURVIVAL; EMBRYONAL CARCINOMA STEM CELLS; EMBRYONIC STEM CELLS; GENE EXPRESSION REGULATION, DEVELOPMENTAL; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; PHENOTYPE; PROTEIN KINASE INHIBITORS; REPRODUCIBILITY OF RESULTS; SMALL MOLECULE LIBRARIES;

EID: 79960533816     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057111406547     Document Type: Article

References (30)

1. Thomson J. A., Itskovitz-Eldor J., Shapiro S. S., Waknitz M. A., Swiergiel J. J., Marshall V. S., Jones J. M. Embryonic Stem Cell Lines Derived from Human Blastocysts. Science. 1998 ; 282: 1145-1147
2. Rajpert-De Meyts E. Developmental Model for the Pathogenesis of Testicular Carcinoma In Situ: Genetic and Environmental Aspects. Hum. Reprod. Update. 2006 ; 12: 303-323
3. Andrews P. W., Damjanov I., Simon D., Banting G. S., Carlin C., Dracopoli N. C., Fogh J. Pluripotent Embryonal Carcinoma Clones Derived from the Human Teratocarcinoma Cell Line Tera-2: Differentiation In Vivo and In Vitro. Lab. Invest. 1984 ; 50: 147-162
4. Draper J. S., Pigott C., Thomson J. A., Andrews P. W. Surface Antigens of Human Embryonic Stem Cells: Changes upon Differentiation in Culture. J. Anat. 2002 ; 200: 249-258
5. Adewumi O., Aflatoonian B., Ahrlund-Richter L., Amit M., Andrews P. W., Beighton G., Bello P. A., Benvenisty N., Berry L. S., Bevan S., et al. Characterization of Human Embryonic Stem Cell Lines by the International Stem Cell Initiative. Nat. Biotechnol. 2007 ; 25: 803-816
6. Andrews P. W. Retinoic Acid Induces Neuronal Differentiation of a Cloned Human Embryonal Carcinoma Cell Line In Vitro. Dev. Biol. 1984 ; 103: 285-293
7. Fenderson B. A., Andrews P. W., Nudelman E., Clausen H., Hakomori S. Glycolipid Core Structure Switching from Globo- to Lacto- and Ganglio-Series during Retinoic Acid-Induced Differentiation of TERA-2-Derived Human Embryonal Carcinoma Cells. Dev. Biol. 1987 ; 122: 21-34
8. Andrews P. W., Gonczol E., Plotkin S. A., Dignazio M., Oosterhuis J. W. Differentiation of TERA-2 Human Embryonal Carcinoma Cells into Neurons and HCMV Permissive Cells: Induction by Agents Other Than Retinoic Acid. Differentiation. 1986 ; 31: 119-126
9. Schuldiner M., Eiges R., Eden A., Yanuka O., Itskovitz-Eldor J., Goldstein R. S., Benvenisty N. Induced Neuronal Differentiation of Human Embryonic Stem Cells. Brain Res. 2001 ; 913: 201-205
10. Aflatoonian B., Ruban L., Shamsuddin S., Baker D., Andrews P., Moore H. Generation of Sheffield (Shef) Human Embryonic Stem Cell Lines Using a Microdrop Culture System. In Vitro Cell Dev. Biol. Anim. 2010 ; 46: 236-241
11. Shevinsky L. H., Knowles B. B., Damjanov I., Solter D. Monoclonal Antibody to Murine Embryos Defines a Stage-Specific Embryonic Antigen Expressed on Mouse Embryos and Human Teratocarcinoma Cells. Cell. 1982 ; 30: 697-705
12. Kannagi R., Cochran N. A., Ishigami F., Hakomori S., Andrews P. W., Knowles B. B., Solter D. Stage-Specific Embryonic Antigens (SSEA-3 and -4) Are Epitopes of a Unique Globo-Series Ganglioside Isolated from Human Teratocarcinoma Cells. EMBO J. 1983 ; 2: 2355-2361
13. Andrews P. W., Banting G., Damjanov I., Arnaud D., Avner P. Three Monoclonal Antibodies Defining Distinct Differentiation Antigens Associated with Different High Molecular Weight Polypeptides on the Surface of Human Embryonal Carcinoma Cells. Hybridoma. 1984 ; 3: 347-361
14. Kohler G., Milstein C. Continuous Cultures of Fused Cells Secreting Antibody of Predefined Specificity. Nature. 1975 ; 256: 495-497
15. Zhang J. H., Chung T. D., Oldenburg K. R. A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays. J. Biomol. Screen. 1999 ; 4: 67-73
16. Livak K. J., Schmittgen T. D. Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2001 ; 25: 402-408
17. Ying Q. L., Wray J., Nichols J., Batlle-Morera L., Doble B., Woodgett J., Cohen P., Smith A. The Ground State of Embryonic Stem Cell Self-Renewal. Nature. 2008 ; 453: 519-523
18. Paling N. R., Wheadon H., Bone H. K., Welham M. J. Regulation of Embryonic Stem Cell Self-Renewal by Phosphoinositide 3-Kinase-Dependent Signaling. J. Biol. Chem. 2004 ; 279: 48063-48070
19. Bain J., Plater L., Elliott M., Shpiro N., Hastie C. J., McLauchlan H., Klevernic I., Arthur J. S., Alessi D. R., Cohen P. The Selectivity of Protein Kinase Inhibitors: A Further Update. Biochem. J. 2007 ; 408: 297-315
20. Bain J., McLauchlan H., Elliott M., Cohen P. The Specificities of Protein Kinase Inhibitors: An Update. Biochem. J. 2003 ; 371: 199-204
21. Andrews P. W., Goodfellow P. N., Shevinsky L. H., Bronson D. L., Knowles B. B. Cell-Surface Antigens of a Clonal Human Embryonal Carcinoma Cell Line: Morphological and Antigenic Differentiation in Culture. Int. J. Cancer. 1982 ; 29: 523-531
22. Andrews P. W., Matin M. M., Bahrami A. R., Damjanov I., Gokhale P., Draper J. S. Embryonic Stem (ES) Cells and Embryonal Carcinoma (EC) Cells: Opposite Sides of the Same Coin. Biochem. Soc. Trans. 2005 ; 33: 1526-1530
23. Gschwendt M., Müller H. J., Kielbassa K., Zang R., Kittstein W., Rincke G., Marks F. Rottlerin, a Novel Protein Kinase Inhibitor. Biochem. Biophys. Res. Commun. 1994 ; 199: 93-98
24. Soltoff S. P. Rottlerin: An Inappropriate and Ineffective Inhibitor of PKCdelta. Trends Pharmacol. Sci. 2007 ; 28: 453-458
25. Tonge P. D., Andrews P. W. Masters J. R. Palsson B. O. Thomson J. A., ed. New York: Springer ; 2007: 235-253.
26. Watanabe K., Ueno M., Kamiya D., Nishiyama A., Matsumura M., Wataya T., Takahashi J. B., Nishikawa S., Nishikawa S., Muguruma K., et al. A ROCK Inhibitor Permits Survival of Dissociated Human Embryonic Stem Cells. Nat. Biotechnol. 2007 ; 25: 681-686
27. Barbaric I., Gokhale P. J., Jones M., Glen A., Baker D., Andrews P. W. Novel Regulators of Stem Cell Fates Identified by a Multivariate Phenotype Screen of Small Compounds on Human Embryonic Stem Cell Colonies. Stem Cell Res. 2010 ; 5: 104-119
28. Chen G., Hou Z., Gulbranson D. R., Thomson J. A. Actin-Myosin Contractility Is Responsible for the Reduced Viability of Dissociated Human Embryonic Stem Cells. Cell Stem Cell. 2010 ; 7: 240-248
29. Aksoy I., Sakabedoyan C., Bourillot P. Y., Malashicheva A. B., Mancip J., Knoblauch K., Afanassieff M., Savatier P. Self-Renewal of Murine Embryonic Stem Cells Is Supported by the Serine/Threonine Kinases Pim-1 and Pim-3. Stem Cells. 2007 ; 25: 2996-3004
30. Chopra P., Sethi G., Dastidar S. G., Ray A. Polo-Like Kinase Inhibitors: An Emerging Opportunity for Cancer Therapeutics. Expert Opin. Investig. Drugs. 2010 ; 19: 27-43