Heat-induced antigen retrieval in immunohistochemistry for estrogen and progesterone receptors in breast cancer tissues

Chinese Journal of Pathology

Volumn 40, Issue 6, 2011, Pages 406-408

  Li, Xin Jun ^a   Wei, Bing ^a   Chen, Hui Jiao ^a   Zhang, Zhang ^a   Zhang, Hong Ying ^a   Bu, Hong ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

BUFFER; ESTROGEN RECEPTOR; FIXATIVE; PARAFFIN; PROGESTERONE RECEPTOR; TUMOR ANTIGEN;

ARTICLE; BREAST TUMOR; FEMALE; HEAT; HUMAN; IMMUNOHISTOCHEMISTRY; METABOLISM; MICROWAVE RADIATION; STAINING;

ANTIGENS, NEOPLASM; BREAST NEOPLASMS; BUFFERS; FEMALE; FIXATIVES; HOT TEMPERATURE; HUMANS; IMMUNOHISTOCHEMISTRY; MICROWAVES; PARAFFIN EMBEDDING; RECEPTORS, ESTROGEN; RECEPTORS, PROGESTERONE; STAINING AND LABELING;

EID: 79960500060     PISSN: 05295807     EISSN: None     Source Type: Journal    
DOI: 10.3760/cma.j.issn.0529-5807.2011.06.011     Document Type: Article

References (9)

1. Rhodes A, Jasani B, Barnes DM, et al. Reliability of immunohistochemical demonstration of oestrogen receptors in routine practice: interlaboratory variance in the sensitivity of detection and evaluation of scoring systems. J Clin Pathol, 2000, 53(2): 125-130. (Pubitemid 30075056)
2. Leake R, Barnes D, Pinder S, et al. Immunohistochemical detection of steroid receptors in breast cancer: a working protocol. UK Receptor Group, UK NEQAS, the Scottish Breast Cancer Pathology Group, and The Receptor and Biomarker Study Group of the EORTC. J Clin Pathol, 2000, 53(8) : 634-635. (Pubitemid 30640178)
3. Rhodes A, Jasani B, Balaton AJ, et al. Immunohistochemical demonstration of oestrogen and progesterone receptors: correlation of standards achieved on in house tumours with that achieved on external quality assessment material in over 150 laboratories from 26 countries. J Clin Pathol, 2000, 53(4): 292-301. (Pubitemid 30180402)
4. Shi SR, Cote RJ, Taylor CR. Antigen retrieval immunohistochemistry: past, present, and future. J Histochem Cytochem, 1997, 45(3): 327-343. (Pubitemid 27120589)
5. Kajiya H, Takekoshi S, Takei M, et al. Selection of buffer pH by the isoelectric point of the antigen for the efficient heat-induced epitope retrieval: re-appraisal for nuclear protein pathobiology. Histochem Cell Biol, 2009, 132(6) :659-667.
6. Rhodes A, Jasani B, Balaton AJ, et al. Study of interlaboratory reliability and reproducibility of estrogen and progesterone receptor assays in Europe. Documentation of poor reliability and identification of insufficient microwave antigen retrieval time as a major contributory element of unreliable assays. Am J Clin Pathol, 2001, 115(l):44-58.
7. von Wasielewski R, Mengel M, Wiese B, et al. Tissue array technology for testing interlaboratory and interobserver reproducibility of immunohistochemical estrogen receptor analysis in a large multicenter trial. Am J Clin Pathol, 2002, 118(5) : 675-682. (Pubitemid 37046311)
8. Neves JI, Begnami MD, Arias V, et al. Antigen retrieval methods and estrogen receptor immunoexpression using 1D5 antibody: a comparative study. Int J Surg Pathol, 2005, 13(4) : 353-357. (Pubitemid 41688597)
9. Hammond ME, Hayes DF, Dowselt M, et al. American Society of Clinical Oncology/College of American Pathologists guideline recommendations for immunohistochemical testing of estrogen and progesterone receptors in breast cancer. Arch Pathol Lab Med, 2010, 134(6): 907-922.