Functional analysis of luxS in Streptococcus suis reveals a key role in biofilm formation and virulence

Veterinary Microbiology

Volumn 152, Issue 1-2, 2011, Pages 151-160

  Wang, Yang ^a,b   Zhang, Wei ^a   Wu, Zongfu ^a   Zhu, Xianglei ^a   Lu, Chengping ^a  

^a NANJING AGRICULTURAL UNIVERSITY   (China)

^b HENAN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Biofilm formation; LuxS gene; Streptococcus suis; Virulence

Indexed keywords

BACTERIAL ENZYME; CARBAMOYL PHOSPHATE SYNTHASE; ELONGATION FACTOR; FBPS PROTEIN; GLUTAMATE DEHYDROGENASE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; MULTIDRUG RESISTANCE PROTEIN; S RIBOSYLHOMOCYSTEINASE; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ARTICLE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM ADHERENCE; BIOFILM; CONTROLLED STUDY; DELETION MUTANT; GENE EXPRESSION; GENE INACTIVATION; GENETIC TRANSCRIPTION; HEMOLYSIS; HUMAN; HUMAN CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STREPTOCOCCUS SUIS; ZEBRA FISH;

ANIMALS; BACTERIAL ADHESION; BACTERIAL PROTEINS; BIOFILMS; CARBON-SULFUR LYASES; CELL LINE; GENE DELETION; GENETIC COMPLEMENTATION TEST; HEMOLYSIS; HUMANS; STREPTOCOCCUS SUIS; TRANSCRIPTION, GENETIC; VIRULENCE; VIRULENCE FACTORS; ZEBRAFISH;

BACTERIA (MICROORGANISMS); DANIO RERIO; STREPTOCOCCUS SUIS; SUIDAE;

EID: 79960414736     PISSN: 03781135     EISSN: 18732542     Source Type: Journal    
DOI: 10.1016/j.vetmic.2011.04.029     Document Type: Article

References (38)

1. Ahmed N.A., Petersen F.C., Scheie A.A. Biofilm formation and autoinducer-2 signaling in Streptococcus intermedius: role of thermal and pH factors. Oral Microbiology and Immunology 2008, 23:492-497.
2. Asanuma N., Yoshii T., Hino T. Molecular characterization and transcription of the luxS gene that encodes LuxS autoinducer 2 synthase in Streptococcus bovis. Current Microbiology 2004, 49:366-371.
3. Bassler B.L., Wright M., Showalter R.E., Silverman M.R. Intercellular signalling in Vibrio harveyi: sequence and function of genes regulating expression of luminescence. Molecular Microbiology 1993, 9:773-786.
4. Bhagwat S.P., Nary J., Burne R.A. Effects of mutating putative two-component systems on biofilm formation by Streptococcus mutans UA159. FEMS Microbiology Letters 2001, 205:225-230.
5. Gonzalez J.E., Keshavan N.D. Messing with bacterial quorum sensing. Microbiology and Molecular Biology Reviews 2006, 70:859-875.
6. Gottschalk M.G., Lacouture S., Dubreuil J.D. Characterization of Streptococcus suis capsular type 2 haemolysin. Microbiology (Reading, England) 1995, 141(Pt 1):189-195.
7. Hall-Stoodley L., Costerton J.W., Stoodley P. Bacterial biofilms: from the natural environment to infectious diseases. Nature Reviews. Microbiology 2004, 2:95-108.
8. Han X., Lu C. Biological activity and identification of a peptide inhibitor of LuxS from Streptococcus suis serotype 2. FEMS Microbiology Letters 2009, 294:16-23.
9. Han X.G., Lu C.P. In vitro biosynthesis of autoinducer 2 of Steptococcus suis Serotype 2 using recombinant LuxS and Pfs. Enzyme and Microbial Technology 2009, 44:40-45.
10. Joyce E.A., Kawale A., Censini S., Kim C.C., Covacci A., Falkow S. LuxS is required for persistent pneumococcal carriage and expression of virulence and biosynthesis genes. Infection and Immunity 2004, 72:2964-2975.
11. Kendall M.M., Sperandio V. Quorum sensing by enteric pathogens. Current Opinion in Gastroenterology 2007, 23:10-15.
12. Livak K.J., Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 2001, 25:402-408.
13. Lyon W.R., Madden J.C., Levin J.C., Stein J.L., Caparon M.G. Mutation of luxS affects growth and virulence factor expression in Streptococcus pyogenes. Molecular Microbiology 2001, 42:145-157.
14. Merritt J., Qi F., Goodman S.D., Anderson M.H., Shi W. Mutation of luxS affects biofilm formation in Streptococcus mutans. Infection and Immunity 2003, 71:1972-1979.
15. Miller M.B., Bassler B.L. Quorum sensing in bacteria. Annual Review of Microbiology 2001, 55:165-199.
16. Parker D., Soong G., Planet P., Brower J., Ratner A.J., Prince A. The NanA neuraminidase of Streptococcus pneumoniae is involved in biofilm formation. Infection and Immunity 2009, 77:3722-3730.
17. Pecharki D., Petersen F.C., Scheie A.A. LuxS and expression of virulence factors in Streptococcus intermedius. Oral Microbiology and Immunology 2008, 23:79-83.
18. Reed L.J., Muench H. A simple method of estimating fifty percent endpoints. American Journal of Hygeine 1938, 27:493-497.
19. Rickard A.H., Palmer R.J., Blehert D.S., Campagna S.R., Semmelhack M.F., Egland P.G., Bassler B.L., Kolenbrander P.E. Autoinducer 2: a concentration-dependent signal for mutualistic bacterial biofilm growth. Molecular Microbiology 2006, 60:1446-1456.
20. Sekizaki T., Otani Y., Osaki M., Takamatsu D., Shimoji Y. Evidence for horizontal transfer of SsuDAT1I restriction-modification genes to the Streptococcus suis genome. Journal of Bacteriology 2001, 183:500-511.
21. Si Y., Yuan F., Chang H., Liu X., Li H., Cai K., Xu Z., Huang Q., Bei W., Chen H. Contribution of glutamine synthetase to the virulence of Streptococcus suis serotype 2. Veterinary Microbiology 2009, 139:80-88.
22. Smith H.E., de Vries R., van't Slot R., Smits M.A. The cps locus of Streptococcus suis serotype 2: genetic determinant for the synthesis of sialic acid. Microbial Pathogenesis 2000, 29:127-134.
23. Staats J.J., Feder I., Okwumabua O., Chengappa M.M. Streptococcus suis: past and present. Veterinary Research Communications 1997, 21:381-407.
24. Staats J.J., Plattner B.L., Nietfeld J., Dritz S., Chengappa M.M. Use of ribotyping and hemolysin activity to identify highly virulent Streptococcus suis type 2 isolates. Journal of Clinical Microbiology 1998, 36:15-19.
25. Stroeher U.H., Paton A.W., Ogunniyi A.D., Paton J.C. Mutation of luxS of Streptococcus pneumoniae affects virulence in a mouse model. Infection and Immunity 2003, 71:3206-3212.
26. Sun L.Y., Fan H.J., Lu C.P. Determination of fibronectin-binding region of FBPS of Streptococcus suis type 2. Wei Sheng Wu Xue Bao 2005, 45:753-756.
27. Takamatsu D., Osaki M., Sekizaki T. Construction and characterization of Streptococcus suis-Escherichia coli shuttle cloning vectors. Plasmid 2001, 45:101-113.
28. Takamatsu D., Osaki M., Sekizaki T. Thermosensitive suicide vectors for gene replacement in Streptococcus suis. Plasmid 2001, 46:140-148.
29. Vendeville A., Winzer K., Heurlier K., Tang C.M., Hardie K.R. Making 'sense' of metabolism: autoinducer-2, LuxS and pathogenic bacteria. Nature Reviews. Microbiology 2005, 3:383-396.
30. Wang Y., Zhang W., Wu Z., Lu C. Reduced virulence is an important characteristic of biofilm infection of Streptococcus suis. FEMS microbiology letters 2011, 316:36-43.
31. Wen Z.T., Burne R.A. LuxS-mediated signaling in Streptococcus mutans is involved in regulation of acid and oxidative stress tolerance and biofilm formation. Journal of Bacteriology 2004, 186:2682-2691.
32. Winzer K., Hardie K.R., Williams P. LuxS and autoinducer-2: their contribution to quorum sensing and metabolism in bacteria. Advances in Applied Microbiology 2003, 53:291-396.
33. Wu Z., Zhang W., Lu Y., Lu C. Transcriptome profiling of zebrafish infected with Streptococcus suis. Microbial Pathogenesis 2010, 48:178-187.
34. Xavier K.B., Bassler B.L. LuxS quorum sensing: more than just a numbers game. Current Opinion in Microbiology 2003, 6:191-197.
35. Xu L., Li H., Vuong C., Vadyvaloo V., Wang J., Yao Y., Otto M., Gao Q. Role of the luxS quorum-sensing system in biofilm formation and virulence of Staphylococcus epidermidis. Infection and Immunity 2006, 74:488-496.
36. Yoshida A., Ansai T., Takehara T., Kuramitsu H.K. LuxS-based signaling affects Streptococcus mutans biofilm formation. Applied and Environmental Microbiology 2005, 71:2372-2380.
37. Zhang H., Fan H., Lu C. Identification of a novel virulence-related gene in Streptococcus suis type 2 strains. Current Microbiology 2010, 61:494-499.
38. Zhu C., Feng S., Sperandio V., Yang Z., Thate T.E., Kaper J.B., Boedeker E.C. The possible influence of LuxS in the in vivo virulence of rabbit enteropathogenic Escherichia coli. Veterinary Microbiology 2007, 125:313-322.