Analysis of deoxyribonucleotide pools in human cancer cell lines using a liquid chromatography coupled with tandem mass spectrometry technique

Biochemical Pharmacology

Volumn 82, Issue 4, 2011, Pages 411-417

  Zhang, Wei ^a   Tan, Shenglan ^a,b   Paintsil, Elijah ^a   Dutschman, Ginger E ^a   Gullen, Elizabeth A ^a   Chu, Edward ^a   Cheng, Yung Chi ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b CENTRAL SOUTH UNIVERSITY   (China)

Author keywords

Aphidicolin; Deoxyribonucleotides; Hydroxyurea; Hypoxia; LC MS MS; Ribonucleotides

Indexed keywords

APHIDICOLIN; DEOXYRIBONUCLEOTIDE; HYDROXYUREA;

ARTICLE; CALIBRATION; CANCER CELL; CELL FUNCTION; CELL SIZE; CELL VOLUME; CONTROLLED STUDY; DRUG EFFECT; HUMAN; HUMAN CELL; HYPOXIA; LIQUID CHROMATOGRAPHY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; TANDEM MASS SPECTROMETRY;

CARCINOMA, HEPATOCELLULAR; CELL LINE, TUMOR; CHROMATOGRAPHY, LIQUID; DEOXYRIBONUCLEOTIDES; HEP G2 CELLS; HUMANS; LEUKEMIA, T-CELL; LIVER NEOPLASMS; LUNG NEOPLASMS; PANCREATIC NEOPLASMS; TANDEM MASS SPECTROMETRY;

EID: 79960357486     PISSN: 00062952     EISSN: 18732968     Source Type: Journal    
DOI: 10.1016/j.bcp.2011.05.009     Document Type: Article

References (23)

1. C. Fagny, M. Vandevelde, M. Svoboda, and P. Robberecht Ribonucleotide reductase and thymidine phosphorylation: two potential targets of azodicarbonamide Biochem Pharmacol 64 2002 451 456 (Pubitemid 34874847)
2. P. Chen, and Y. Chai Dynamics of cAMP/cGMP in patients under a stress state Chin J Traumatol 5 2002 115 117 (Pubitemid 43026808)
3. Z. Horvath, T. Hochtl, W. Bauer, M. Fritzer-Szekeres, H.L. Elford, and T. Szekeres Synergistic cytotoxicity of the ribonucleotide reductase inhibitor didox (3,4-dihydroxy-benzohydroxamic acid) and the alkylating agent carmustine (BCNU) in 9L rat gliosarcoma cells and DAOY human medulloblastoma cells Cancer Chemother Pharmacol 54 2004 139 145 (Pubitemid 39044243)
4. G. Hennere, F. Becher, A. Pruvost, C. Goujard, J. Grassi, and H. Benech Liquid chromatography-tandem mass spectrometry assays for intracellular deoxyribonucleotide triphosphate competitors of nucleoside antiretrovirals J Chromatogr B Analyt Technol Biomed Life Sci 789 2003 273 281 (Pubitemid 36549197)
5. A.W. Solter, and R.E. Handschumacher A rapid quantitative determination of deoxyribonucleoside triphosphates based on the enzymatic synthesis of DNA Biochim Biophys Acta 174 1969 585 590
6. C.D. Yegian A procedure for the measurement of intracellular deoxyribonucleoside triphosphate pools by thin layer chromatography Anal Biochem 58 1974 231 237
7. E.M. Piall, G.W. Aherne, and V. Marks The quantitative determination of 2′-deoxycytidine-5′-triphosphate in cell extracts by radioimmunoassay Anal Biochem 154 1986 276 281 (Pubitemid 16063370)
8. D.S. Shewach Quantitation of deoxyribonucleoside 5′-triphosphates by a sequential boronate and anion-exchange high-pressure liquid chromatographic procedure Anal Biochem 206 1992 178 182
9. S. Cohen, M. Megherbi, L.P. Jordheim, I. Lefebvre, C. Perigaud, and C. Dumontet Simultaneous analysis of eight nucleoside triphosphates in cell lines by liquid chromatography coupled with tandem mass spectrometry J Chromatogr B Analyt Technol Biomed Life Sci 877 2009 3831 3840
10. J. Chi, A. Jayewardene, J. Stone, J.G. Gambertoglio, and F.T. Aweeka A direct determination of thymidine triphosphate concentrations without dephosphorylation in peripheral blood mononuclear cells by LC/MS/MS J Pharm Biomed Anal 26 2001 829 836 (Pubitemid 32971694)
11. E. Paintsil, G.E. Dutschman, R. Hu, S.P. Grill, W. Lam, and M. Baba Intracellular metabolism and persistence of the anti-human immunodeficiency virus activity of 2′,3′-didehydro-3′-deoxy-4′- ethynylthymidine, a novel thymidine analog Antimicrob Agents Chemother 51 2007 3870 3879 (Pubitemid 350057783)
12. P. Ferraro, V. Bianchi, M.R. Biasin, and L. Celotti Deoxynucleotide pools and DNA synthesis in resting and PHA-stimulated human lymphocytes treated with mutagens Exp Cell Res 199 1992 349 354
13. H. Iwasaki, P. Huang, M.J. Keating, and W. Plunkett Differential incorporation of ara-C, gemcitabine, and fludarabine into replicating and repairing DNA in proliferating human leukemia cells Blood 90 1997 270 278 (Pubitemid 27276571)
14. L. Coulier, R. Bas, S. Jespersen, E. Verheij, M.J. van der Werf, and T. Hankemeier Simultaneous quantitative analysis of metabolites using ion-pair liquid chromatography-electrospray ionization mass spectrometry Anal Chem 78 2006 6573 6582 (Pubitemid 44413611)
15. Z. Cai, F. Song, and M.S. Yang Capillary liquid chromatographic-high- resolution mass spectrometric analysis of ribonucleotides J Chromatogr A 976 2002 135 143 (Pubitemid 35232515)
16. C.J. Van Moorsel, K. Smid, D.A. Voorn, A.M. Bergman, H.M. Pinedo, and G.J. Peters Effect of gemcitabine and cis-platinum combinations on ribonucleotide and deoxyribonucleotide pools in ovarian cancer cell lines Int J Oncol 22 2003 201 207
17. A.R. Van Rompay, M. Johansson, and A. Karlsson Phosphorylation of nucleosides and nucleoside analogs by mammalian nucleoside monophosphate kinases Pharmacol Ther 87 2000 189 198
18. A. Kurose, T. Tanaka, X. Huang, F. Traganos, W. Dai, and Z. Darzynkiewicz Effects of hydroxyurea and aphidicolin on phosphorylation of ataxia telangiectasia mutated on Ser 1981 and histone H2AX on Ser 139 in relation to cell cycle phase and induction of apoptosis Cytometry A 69 2006 212 221
19. M. Fukuda, and M. Ohashi Aphidicolin inhibits cell growth by accumulation of G2 cells Cell Biol Int Rep 7 1983 579 585
20. E. Smitskamp-Wilms, H.M. Pinedo, G. Veerman, V.W. Ruiz van Haperen, and G.J. Peters Postconfluent multilayered cell line cultures for selective screening of gemcitabine Eur J Cancer 34 1998 921 926 (Pubitemid 28248507)
21. T.W. Traut Physiological concentrations of purines and pyrimidines Mol Cell Biochem 140 1994 1 22 (Pubitemid 24379884)
22. C.J. van Moorsel, A.M. Bergman, G. Veerman, D.A. Voorn, V.W. Ruiz van Haperen, and J.R. Kroep Differential effects of gemcitabine on ribonucleotide pools of 21 solid tumour and leukaemia cell lines Biochim Biophys Acta 1474 2000 5 12 (Pubitemid 30103526)
23. M.H. Gadsden, E.M. McIntosh, J.C. Game, P.J. Wilson, and R.H. Haynes dUTP pyrophosphatase is an essential enzyme in Saccharomyces cerevisiae EMBO J 12 1993 4425 4431 (Pubitemid 23302057)