Rapid RP-HPLC technique for the determination of phyllanthin as bulk and its quantification in Phyllanthus amarus extract

International Journal of Phytomedicine

Volumn 3, Issue 1, 2011, Pages 115-119

  Alvari, Amene ^a   Rafsanjani, Mehrnaz S Ohadi ^a   Ahmed, Farhan J ^a   Hejazi, Mohd S ^b   Abdin, Malik Z ^a  

^a HAMDARD UNIVERSITY   (India)

^b UNIVERSITY OF TABRIZ   (Iran)

Author keywords

HPLC; Phyllanthin; Phyllanthus amarus; Plant extract

Indexed keywords

LIGNAN DERIVATIVE; PHYLLANTHIN; PHYLLANTHUS AMARUS EXTRACT; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; DRUG DETERMINATION; DRUG SOLUBILITY; LIMIT OF DETECTION; LIMIT OF QUANTITATION; PHYLLANTHUS AMARUS; PROCESS DEVELOPMENT; PROCESS OPTIMIZATION; QUANTITATIVE ANALYSIS; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SENSITIVITY AND SPECIFICITY; ULTRAVIOLET SPECTROPHOTOMETRY; VALIDATION PROCESS;

EID: 79960291114     PISSN: None     EISSN: 09750185     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Thagarajan SP, Thiruneelakantan K, Subramaniam S, Sundaravelu T. Invitro inactivation of HBsAg by Eclipta alba and Phyllanthus niruri Linn. Ind. J. Med. Res. 1982;76:124-130.
2. Thagarajan SP, Subramaniam S, Thirunalasundari T. Effect of Phyllanthus amarus on chronic carriers of Hepatitis B virus. The Lancet. 1988;11:764-766.
3. Venkateswaran PS, Millman I, Blumberg BS. Effect of an extract from Phyllanthus niruri on Hepatitis B and Woodchuck Hepatitis viruses: Invitro and invivo studies, Proc. Natl. Acad. Sci. USA. 1987;84:274-278.
4. Mehrotra R, Rawat S, Kulshreshtha DK. Invitro effect of Phyllanthus amarus on Hepatitis B virus. Ind. J Med. Res. 1991;93:71-73.
5. Jayaram S, Thyagarajan SP. Inhibition of HBsAg secrection from Alexander cell line by Phyllanthus amarus. Indian J. Pathol. Microbiol. 1996;39:211-215.
6. Yeh SF, Hong CY, Huang YL, Liu TY, Choo KB, Chou CK. Effect of an extract from Phyllanthus amarus on Hepatitis B surface antigen gene expression in human hepatoma cells. Antiviral Res. 1993;20:185-192.
7. Lee CD, Ott M, Thayagarajan SP, Shafritz DA, Burk RD, Gupta S. Phyllanthus amarus down regulates Hepatitis B virus mRNA transcription and replication. Eur. J. Clin. Invest. 1996;26:1069-1076.
8. Ott M, Thyagarajan SP, Gupta S. Phyllanthus amarus suppresses Hepatitis B virus by interrupting interaction between HBV enhancer I and cellular transcription factors. Eur. J. Clin. Invest. 1997;27:908-915.
9. Ramakrishnan PN, Murugesan R, Palanichamy S, Murugesh N. Oral hypoglycaemic effect of Phyllanthus niruri Linn. Leaves. Ind.J. Pharmaceu. Sci. 1982;44:10-12.
10. Moshi MJ, Uiso FC, Mahunnah R.L. A study of the effect of Phyllanthus amarus extract on blood glucose in rabbits. Int J. Pharmacog. 1997;35:167-173.
11. Srividya N, Periwal S. Diuretic, hypotensive and hypoglycaemic effect of Phyllanthus amarus. Ind. J. Exp. Biol. 1995;33:861-864.
12. Sivaprakasam K, Yasodha R, Sivanandam G, Veluchamy G. Clinical evaluation of Phyllanthus amarus Schum & Thonn. in Diabetes Mellitus. Seminar on research in Ayurveda and Siddha, CCRAS, New Delhi. 1995;20-22: p17.
13. th:82.
14. 4 induced liver dysfunction. Current Science. 1995;68:1243-1246.
15. Venkateswaran PS, Millman I, Blumberg BS. Effects of an extract from Phyllanthus niruri on Hepatitis B and Woodchuck hepatitis viruses Invitro and Invivo studies Proc. Natl. Acad. Sci. USA. 1987;84:274-278.
16. Calixto JB, Santos AR, Cechinel Filho V, Yunes RA. A review of the plants of the genus Phyllanthus: their chemistry. Pharmacology and therapeutic potential. Med Res Rev. 1998;18:225-258.
17. Shakila A, Rajeswari R. Effect of GA3, certain chemicals and media on germination, growth and yield of Phyllanthus niruri. Plant Arch. 2006;6:121-125.
18. ICH Haromonised Tripartite Guideline, International Conference on Harmonisation on Technical Requirements for Registration of Pharmaceuticals for Human Use, Q2A, Text on Validation of Analytical Procedures, Step 4 of the ICH process, 1994 and Q2B, Validation of Analytical Procedures: Methodology, Step 4 of the ICH process, 1996, ICH Steering Committee.