A novel role of protein tyrosine kinase2 in mediating chloride secretion in human airway epithelial cells

PLoS ONE

Volumn 6, Issue 7, 2011, Pages

  Liang, Lihua ^a   Woodward, Owen M ^a   Chen, Zhaohui ^a   Cotter, Robert ^a   Guggino, William B ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM ACTIVATED CHLORIDE CHANNEL; CALCIUM ION; CHLORIDE CHANNEL; CHLORIDE ION; FOCAL ADHESION KINASE 2; SPIPERONE; TRANSMEMBRANE CONDUCTANCE REGULATOR; UNCLASSIFIED DRUG; CALCIUM; CHLORIDE; GREEN FLUORESCENT PROTEIN; PHOSPHOTYROSINE; PTK2B PROTEIN, HUMAN;

ARTICLE; CALCIUM CELL LEVEL; CHLORIDE TRANSPORT; CONTROLLED STUDY; ENZYME INHIBITION; EPITHELIUM CELL; HUMAN; HUMAN CELL; PROTEIN FUNCTION; RESPIRATORY EPITHELIUM; SIGNAL TRANSDUCTION; ANIMAL; BRONCHUS; CHANNEL GATING; CHO CELL; CRICETULUS; CYTOLOGY; DRUG ANTAGONISM; DRUG EFFECT; ENZYMOLOGY; GENETIC TRANSFECTION; HAMSTER; INTRACELLULAR SPACE; METABOLISM; MOUSE; PHOSPHORYLATION; PROTEIN BINDING; REPRODUCIBILITY; SECRETION;

MUS;

ANIMALS; BRONCHI; CALCIUM; CHLORIDES; CHO CELLS; CRICETINAE; CRICETULUS; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; EPITHELIAL CELLS; FOCAL ADHESION KINASE 2; GREEN FLUORESCENT PROTEINS; HUMANS; INTRACELLULAR SPACE; ION CHANNEL GATING; MICE; PHOSPHORYLATION; PHOSPHOTYROSINE; PROTEIN BINDING; REPRODUCIBILITY OF RESULTS; SPIPERONE; TRANSFECTION;

EID: 79960203340     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0021991     Document Type: Article

References (30)

1. Sheppard DN, Welsh MJ, (1999) Structure and function of the CFTR chloride channel. Physiol Rev 79: S23-S45.
2. Anderson MP, Welsh MJ, (1991) Calcium and cAMP activate different chloride channels in the apical membrane of normal and cystic fibrosis epithelia. Proc Natl Acad Sci U S A 88: 6003-6007.
3. Liang L, MacDonald K, Schwiebert EM, Zeitlin PL, Guggino WB, (2009) Spiperone, identified through compound screening, activates calcium-dependent chloride secretion in the airway. Am J Physiol Cell Physiol 296: C131-C141.
4. Avraham H, Park SY, Schinkmann K, Avraham S, (2000) RAFTK/Pyk2-mediated cellular signalling. Cell Signal 12: 123-133.
5. Schaller MD, (2010) Cellular functions of FAK kinases: insight into molecular mechanisms and novel functions. J Cell Sci 123 (Pt 7): 1007-1013.
6. Defilippi P, Di Stefano P, Cabodi S, (2006) p130Cas: a versatile scaffold in signaling networks. Trends Cell Biol 16: 257-263.
7. Li W, Fan J, Woodley DT, (2001) Nck/Dock: an adapter between cell surface receptors and the actin cytoskeleton. Oncogene 20: 6403-6417.
8. Silva JM, Ezhkova E, Silva J, Heart S, Castillo M, et al. (2009) Cyfip1 is a putative invasion suppressor in epithelial cancers. Cell 137: 1047-1061.
9. Rufanova VA, Alexanian A, Wakatsuki T, Lerner A, Sorokin A, (2009) Pyk2 mediates endothelin-1 signaling via p130Cas/BCAR3 cascade and regulates human glomerular mesangial cell adhesion and spreading. J Cell Physiol 219: 45-56.
10. Giachini FR, Carneiro FS, Lima VV, Carneiro ZN, Carvalho MH, et al. (2008) Pyk2 mediates increased adrenergic contractile responses in arteries from DOCA-salt mice- VASOACTIVE PEPTIDE SYMPOSIUM. J Am Soc Hypertens 2: 431-438.
11. Chien LT, Zhang ZR, Hartzell HC, (2006) Single Cl- channels activated by Ca2+ in Drosophila S2 cells are mediated by bestrophins. J Gen Physiol 128: 247-259.
12. Marshall WS, Watters KD, Hovdestad LR, Cozzi RR, Katoh F, (2009) CFTR Cl- channel functional regulation by phosphorylation of focal adhesion kinase at tyrosine 407 in osmosensitive ion transporting mitochondria rich cells of euryhaline killifish. J Exp Biol 212: 2365-2377.
13. Leysen JE, Niemegeers CJ, Tollenaere JP, Laduron PM, (1978) 272: 168-171 Nature Serotonergic component of neuroleptic receptors.
14. Creese I, Snyder SH, (1978) Dopamine receptor binding of 3H-ADTN (2-amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene) regulated by guanyl nucleotides. Eur J Pharmacol 49: 201-202.
15. Gundlach AL, Largent BL, Snyder SH, (1984) 125I-Spiperone: a novel ligand for D2 dopamine receptors. Life Sci 35: 1981-1988.
16. Leysen JE, Gommeren W, Laduron PM, (1978) Spiperone: a ligand of choice for neuroleptic receptors. 1. Kinetics and characteristics of in vitro binding. Biochem Pharmacol 27: 307-316.
17. Glennon RA, Metwally K, Dukat M, Ismaiel AM, De los AJ, et al. (2002) Ketanserin and spiperone as templates for novel serotonin 5-HT(2A) antagonists. Curr Top Med Chem 2: 539-558.
18. Lu D, Carson DA, (2009) Spiperone enhances intracellular calcium level and inhibits the Wnt signaling pathway. BMC Pharmacol 9: 13.
19. Zheng LT, Hwang J, Ock J, Lee MG, Lee WH, et al. (2008) The antipsychotic spiperone attenuates inflammatory response in cultured microglia via the reduction of proinflammatory cytokine expression and nitric oxide production. J Neurochem 107: 1225-1235.
20. Schlaepfer DD, Hauck CR, Sieg DJ, (1999) Signaling through focal adhesion kinase. Prog Biophys Mol Biol 71: 435-478.
21. Anitei M, Stange C, Parshina I, Baust T, Schenck A, et al. (2010) Protein complexes containing CYFIP/Sra/PIR121 coordinate Arf1 and Rac1 signalling during clathrin-AP-1-coated carrier biogenesis at the TGN. Nat Cell Biol 12: 330-340.
22. Lev S, Moreno H, Martinez R, Canoll P, Peles E, et al. (1995) Protein tyrosine kinase PYK2 involved in Ca(2+)-induced regulation of ion channel and MAP kinase functions. Nature 376: 737-745.
23. Kohno T, Matsuda E, Sasaki H, Sasaki T, (2008) Protein-tyrosine kinase CAKbeta/PYK2 is activated by binding Ca2+/calmodulin to FERM F2 alpha2 helix and thus forming its dimer. Biochem J 410: 513-523.
24. Matsui A, Okigaki M, Amano K, Adachi Y, Jin D, et al. (2007) Central role of calcium-dependent tyrosine kinase PYK2 in endothelial nitric oxide synthase-mediated angiogenic response and vascular function. Circulation 116: 1041-1051.
25. Okigaki M, Davis C, Falasca M, Harroch S, Felsenfeld DP, et al. (2003) Pyk2 regulates multiple signaling events crucial for macrophage morphology and migration. Proc Natl Acad Sci U S A 100: 10740-10745.
26. Dahan D, Evagelidis A, Hanrahan JW, Hinkson DA, Jia Y, et al. (2001) Regulation of the CFTR channel by phosphorylation. Pflugers Arch 443 (Suppl 1): S92-S96.
27. Esen M, Grassmé H, Riethmüller J, Riehle A, Fassbender K, et al. (2001) Invasion of human epithelial cells by Pseudomonas aeruginosa involves src-like tyrosine kinases p60Src and p59Fyn. Infect Immun 69: 281-287.
28. Mohler PJ, Kreda SM, Boucher RC, Sudol M, Stutts MJ, et al. (1999) Yes-associated protein 65 localizes p62 (c-Yes) to the apical compartment of airway epithelia by association with EBP50. J Cell Biol 147: 879-890.
29. Selvaraj NG, Omi E, Gibori G, Rao MC, (2000) Janus kinase 2 (JAK2) regulates prolactin-mediated chloride transport in mouse mammary epithelial cells through tyrosine phosphorylation of Na+-K+-2Cl- cotransporter. Mol Endocrinol 14: 2054-2065.
30. Zeitlin PL, Lu L, Rhim J, Cutting G, Stetten G, et al. (1991) A cystic fibrosis bronchial epithelial cell line: immortalization by adeno-12-SV40 infection. Am J Respir Cell Mol Biol 4: 313-319.