Real-time detection of loop-mediated isothermal amplification reaction on microfluidic chip

5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011

Volumn , Issue , 2011, Pages

  Xu, Ling Yan ^a   Jin, Wei ^a   Zhu, Qiang Yuan ^a   Yang, Wen Xiu ^a   Wu, Qing Qing ^a   Jin, Qin Han ^a   Mu, Ying ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

Dynamics process; Loop mediated isothermal amplification; Microfluidic chips; Real time detection

Indexed keywords

ABSORBANCE MEASUREMENTS; ABSORBANCES; END POINTS; HIGH EFFICIENCY; LOOP-MEDIATED ISOTHERMAL AMPLIFICATION; MAGNESIUM IONS; MICROFLUIDIC CHIP; MICROFLUIDIC CHIPS; OPTIC SENSOR; REAL-TIME DETECTION; SHORT PERIODS; START POINT;

BIOMEDICAL ENGINEERING; FLUIDIC DEVICES; MAGNESIUM; METAL IONS; MICROFLUIDICS; SIGNAL DETECTION;

BIOINFORMATICS;

EID: 79960131500     PISSN: None     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1109/icbbe.2011.5780331     Document Type: Conference Paper

References (13)

1. Y. Mori, M. Kitao, N Tomita, T Notomi, "Real-time turbidimetry of LAMP reaction for quantifying template DNA, " J. Biochem. Biophys. Methods, vol. 59, pp. 145-157, 2004.
2. C. A. Heid, J Stevens, K. J. Livak, P. M. Williams, "Real time quantitative PCR, " Genome Res, vol. 6, pp. 986-994, 1996.
3. R. Higuchi, G. Fockler, G. Dollinger, R. Watson, "Kinetic PCR analysis: real-time monitoring of DNA mplification reaction, " Bio/Technology, vol.11, pp. 1026- 1030, 1993.
4. F. Oehlenschläger, P. Schwille, M. Eigne, " Detection of HIV-1 RNA by nucleic acid sequence-based amplification combined with fluorescence correlation spectroscopy, " Proc Natl Acad Sci U S A, vol .93, pp. 12811 -12816, 1996.
5. G. T. Walker, M. C. Little, J. G. Nadeau, D. D. Shank, " Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system, " Proc Natl Acad Sci U S A, vol. 89, pp. 392-396, 1992.
6. T. Notomi, H. Okayama, H. Masubuchi, Nucleic Acids Res, 12th ed, vol. 28, pp.e63, 2000.
7. X. E. Fang, Y. Y. Liu, J. L. Kong, X. Y.Jiang, "Loop-mediated isothermal amplification integrated on microfluidic chips for point-ofcare quantitative detection of pathogens, " Anal. Chem, vol. 82, pp. 3002-3006, 2010.
8. S. Y. Lee, J. G. Huang, T. L. Chuang, J.C. Sheu, Y. K. Chuan, M. Holl, D. R. Meldrum, C. N. Lee, C. W Lin, Sens. Actuators, B 2008, pp. 133, 493-501.
9. M. C. Li, "The manufacturing technology of electrophoresis microfludic chip integrated optical fibers, " Science Bulletin, vol. 52, pp. 358-360, 2007.
10. B. Su, D. F. Cui, C. C. Liu, H. Y. Cai, Z. X. Geng, "The detection system design of microfluidic chip with optical fiber, "Chinese Journal of Analytical Chemistry, vol. 34, pp. S295-S298, 2006.
11. S. L. Cui, Optical Fiber Sensor Principles and Applied Technologies. Harbin: Harbi Engineering University Press, 1995.
12. H. F. Li, J. M. Lin, R. G. Su, K. Uchiyama, T. Hobo, Electrophoresis, 12 th ed, vol. 25, pp. 1907-1915, 2004.
13. D. M. Wu, Optical Fiber Communications Principles and Technologies. Beijing: Science Press, 2004.