Measuring Glutamate Receptor Activation-Induced Apoptotic Cell Death in Ischemic Rat Retina Using the TUNEL Assay

Methods in Molecular Biology

Volumn 740, Issue , 2011, Pages 149-156

  Ju, Won Kyu ^a   Kim, Keun Young ^b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b NONE

Author keywords

Apoptotic cell death; Excitotoxicity; Glutamate receptor; Ischemia; MK801; Retina; TUNEL

Indexed keywords

GLUTAMATE RECEPTOR;

ANIMAL; APOPTOSIS; ARTICLE; FEMALE; ISCHEMIA; METABOLISM; METHODOLOGY; NICK END LABELING; PATHOLOGY; RAT; RETINA; SPRAGUE DAWLEY RAT; STAINING;

ANIMALS; APOPTOSIS; FEMALE; IN SITU NICK-END LABELING; ISCHEMIA; RATS; RATS, SPRAGUE-DAWLEY; RECEPTORS, GLUTAMATE; RETINA; STAINING AND LABELING;

EID: 79960116416     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-108-6_16     Document Type: Chapter

References (18)

1. Ju, W. K., Lindsey, J. D., Angert, M., Patel, A., and Weinreb, R. N. (2008) Glutamate receptor activation triggers OPA1 release and induces apoptotic cell death in ischemic rat retina, Mol Vis 14, 2629–2638.
2. Buchi, E. R. (1992) Cell death in the rat retina after a pressure-induced ischaemia-reperfusion insult: an electron microscopic study. I. Ganglion cell layer and inner nuclear layer, Exp Eye Res 55, 605–613.
3. Nickells, R. W. (1996) Retinal ganglion cell death in glaucoma: the how, the why, and the maybe, J Glaucoma 5, 345–356.
4. Rosenbaum, D. M., Rosenbaum, P. S., Gupta, H., Singh, M., Aggarwal, A., Hall, D. H., Roth, S., and Kessler, J. A. (1998) The role of the p53 protein in the selective vulnerability of the inner retina to transient ischemia, Invest Ophthalmol Vis Sci 39, 2132–2139.
5. Joo, C. K., Choi, J. S., Ko, H. W., Park, K. Y., Sohn, S., Chun, M. H., Oh, Y. J., and Gwag, B. J. (1999) Necrosis and apoptosis after retinal ischemia: involvement of NMDA-mediated excitotoxicity and p53, Invest Ophthalmol Vis Sci 40, 713–720.
6. Ju, W. K., Kim, K. Y., Hofmann, H. D., Kim, I. B., Lee, M. Y., Oh, S. J., and Chun, M. H. (2000) Selective neuronal survival and upregulation of PCNA in the rat inner retina following transient ischemia, J Neuropathol Exp Neurol 59, 241–250.
7. Zhang, Y., Cho, C. H., Atchaneeyasakul, L. O., McFarland, T., Appukuttan, B., and Stout, J. T. (2005) Activation of the mitochondrial apoptotic pathway in a rat model of central retinal artery occlusion, Invest Ophthalmol Vis Sci 46, 2133–2139.
8. Russo, R., Rotiroti, D., Tassorelli, C., Nucci, C., Bagetta, G., Bucci, M. G., Corasaniti, M. T., and Morrone, L. A. (2009) Identification of novel pharmacological targets to minimize excitotoxic retinal damage, Int Rev Neurobiol 85, 407–423.
9. Szabo, M. E., Droy-Lefaix, M. T., Doly, M., Carre, C., and Braquet, P. (1991) Ischemia and reperfusion-induced histologic changes in the rat retina. Demonstration of a free radicalmediated mechanism, Invest Ophthalmol Vis Sci 32, 1471–1478.
10. Hayreh, S. S., and Weingeist, T. A. (1980) Experimental occlusion of the central artery of the retina. IV: Retinal tolerance time to acute ischaemia, Br J Ophthalmol 64, 818–825.
11. Osborne, N. N., Larsen, A., and Barnett, N. L. (1995) Influence of excitatory amino acids and ischemia on rat retinal choline acetyltransferase-containing cells, Invest Ophthalmol Vis Sci 36, 1692–1700.
12. Lombardi, G., and Moroni, F. (1994) Glutamate receptor antagonists protect against ischemia-induced retinal damage, Eur J Pharmacol 271, 489–495.
13. Choi, D. W. (1996) Ischemia-induced neuronal apoptosis, Curr Opin Neurobiol 6, 667–672.
14. Brandstatter, J. H., Koulen, P., and Wassle, H. (1998) Diversity of glutamate receptors in the mammalian retina, Vision Res 38, 1385–1397.
15. Brandstatter, J. H., and Hack, I. (2001) Localization of glutamate receptors at a complex synapse. The mammalian photoreceptor synapse, Cell Tissue Res 303, 1–14.
16. Melino, G. (2001) The Sirens’ song, Nature 412, 23.
17. Wyllie, A. H., Kerr, J. F., and Currie, A. R. (1980) Cell death: the significance of apoptosis, Int Rev Cytol 68, 251–306.
18. Kroemer, G., Galluzzi, L., Vandenabeele, P., Abrams, J., Alnemri, E. S., Baehrecke, E. H., Blagosklonny, M. V., El-Deiry, W. S., Golstein, P., Green, D. R., Hengartner, M., Knight, R. A., Kumar, S., Lipton, S. A., Malorni, W., Nunez, G., Peter, M. E., Tschopp, J., Yuan, J., Piacentini, M., Zhivotovsky, B., and Melino, G. (2009) Classification of cell death: recommendations of the Nomenclature Committee on Cell Death 2009, Cell Death Differ 16, 3–11.