BTI1, an azoreductase with pH-dependent substrate specificity

Applied and Environmental Microbiology

Volumn 77, Issue 12, 2011, Pages 4223-4225

  Johansson, Hans E ^a   Johansson, Mary K ^a   Wong, Albert C ^a   Armstrong, Eliana S ^a,b   Peterson, Erik J ^a,c   Grant, Richard E ^a,d   Roy, Margaret A ^a,e   Reddington, Mark V ^a   Cook, Ronald M ^a  

^a Biosearch Technologies Inc   (United States)

^b Achaogen Inc   (United States)

^c Department of Environmental and Radiological Health Sciences   (United States)

^d UNIVERSITY OF WISCONSIN MADISON   (United States)

^e Complete Genomics Inc ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AZO BOND; AZOREDUCTASE; JANUS GREEN B; METHYL ORANGE; METHYL RED; OPTIMAL REDUCTIONS; PH-DEPENDENT; SUBSTRATE SPECIFICITY;

AZO DYES;

AZO COMPOUND; AZO REDUCTASE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; OXIDOREDUCTASE;

DYE; ENZYME ACTIVITY; FLUORESCENCE; PH; SUBSTRATE;

ARTICLE; ENZYME SPECIFICITY; METABOLISM; PH;

AZO COMPOUNDS; HYDROGEN-ION CONCENTRATION; NADH, NADPH OXIDOREDUCTASES; NADP; SUBSTRATE SPECIFICITY;

EID: 79960100481     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02289-10     Document Type: Article

References (13)

1. Binter, A., et al. 2009. A single intersubunit salt bridge affects oligomerization and catalytic activity in a bacterial quinone reductase. FEBS J. 276: 5263-5274.
2. Chen, H., S. L. Hopper, and C. E. Cerniglia. 2005. Biochemical and molecular characterization of an azoreductase from Staphylococcus aureus, a tetrameric NADPH-dependent flavoprotein. Microbiology 151: 1433-1441.
3. Chen, H., R. F. Wang, and C. E. Cerniglia. 2004. Molecular cloning, overexpression, purification, and characterization of an aerobic FMN-dependent azoreductase from Enterococcus faecalis. Protein Expr. Purif. 34:302-310.
4. Deller, S., et al. 2006. Characterization of a thermostable NADPH:FMN oxidoreductase from the mesophilic bacterium Bacillus subtilis. Biochemistry 45:7083-7091.
5. Johansson, M. K. 2006. Choosing reporter-quencher pairs for efficient quenching through formation of intramolecular dimers. Methods Mol. Biol. 335:17-29.
6. Liu, G., et al. 2007. Azoreductase from Rhodobacter sphaeroides AS1.1737 is a flavodoxin that also functions as nitroreductase and flavin mononucleotide reductase. Appl. Microbiol. Biotechnol. 76:1271-1279.
7. Liu, G., et al. 2008. Site-directed mutagenesis of substrate binding sites of azoreductase from Rhodobacter sphaeroides. Biotechnol. Lett. 30:869-875.
8. Pricelius, S., et al. 2007. Enzymatic reduction of azo and indigoid compounds. Appl. Microbiol. Biotechnol. 77:321-327.
9. Pricelius, S., et al. 2007. Enzymatic reduction and oxidation of fibre-bound azo-dyes. Enzyme Microbial Technol. 40:1732-1738.
10. Sugiura, W., T. Yoda, T. Matsuba, Y. Tanaka, and Y. Suzuki. 2006. Expression and characterization of the genes encoding azoreductases from Bacillus subtilis and Geobacillus stearothermophilus. Biosci. Biotechnol. Biochem. 70: 1655-1665.
11. Suzuki, Y., T. Yoda, A. Ruhul, and W. Sugiura. 2001. Molecular cloning and characterization of the gene coding for azoreductase from Bacillus sp. OY1-2 isolated from soil. J. Biol. Chem. 276:9059-9065.
12. Wu, J. T., L. H. Wu, and J. A. Knight. 1986. Stability of NADPH: effect of various factors on the kinetics of degradation. Clin. Chem. 32:314-319.
13. Yan, B., et al. 2004. Expression and characteristics of the gene encoding azoreductase from Rhodobacter sphaeroides AS1.1737. FEMS Microbiol. Lett. 236:129-136.