Effect of plant growth regulators on callus, cell suspension and cell line selection for flavonoid production from pegaga (centella asiatica L. urban)

American Journal of Biochemistry and Biotechnology

Volumn 6, Issue 4, 2010, Pages 284-299

  Tan, Suat Hian ^a,b   Musa, Radzali ^a   Ariff, Arbakariya ^a   Maziah, Mahmood ^a  

^a UNIVERSITY PUTRA MALAYSIA   (Malaysia)

^b UCSI UNIVERSITY   (Malaysia)

Author keywords

Callus induction; Centella asiatica; Controll cells; Dry Weight (DW); Elite cell; Flavonoid production; Luteolin; Napthaleneacetic Acid (NAA); Plant Growth Regulators (PRGs); Suspension cultures; Tissue culture

Indexed keywords

CALLUS INDUCTION; CENTELLA ASIATICA; CONTROLL CELLS; DRY WEIGHT; ELITE CELL; FLAVONOID PRODUCTION; LUTEOLIN; NAPTHALENEACETIC ACIDS; PLANT GROWTH REGULATORS; SUSPENSION CULTURES;

CELLS; CYTOLOGY; ECOLOGY; FATTY ACIDS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PHENOLS; PLANTS (BOTANY); PRODUCTION ENGINEERING; SELF ASSEMBLY; SUGAR (SUCROSE); TISSUE; TISSUE CULTURE;

GROWTH KINETICS;

6 BENZYL ADENINE; ACETIC ACID; ADENINE; DICAMBA; FLAVONOID; INDOLEBUTYRIC ACID; KINETIN; LUTEOLIN; NAPTHALENEACETIC ACID; PANTOTHENIC ACID; PHYTOHORMONE; PICLORAM; QUERCETIN; RUTOSIDE; SUCROSE; UNCLASSIFIED DRUG;

ARTICLE; BIOMASS; CALLUS (PLANT); CALLUS CULTURE; CELL LINE; CELL PROLIFERATION; CELL SUSPENSION; CENTELLA ASIATICA; CONTROLLED STUDY; DRY WEIGHT; EXPLANT; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; MURASHIGE AND SKOOG MEDIUM; NONHUMAN; PLANT LEAF; SUSPENSION CELL CULTURE;

CENTELLA ASIATICA;

EID: 79960085282     PISSN: 15533468     EISSN: None     Source Type: Journal    
DOI: 10.3844/ajbbsp.2010.284.299     Document Type: Article

References (59)

1. Abbasi, B.H., C.L. Tian, S.J. Murch, P.K. Saxena and C.Z. Liu, 2007. Light-enhanced caffeic acid derivatives biosynthesis in hairy root cultures of echinacea purpurea. Plant Cell Rep., 26: 1367-1372. DOI: 10.1007/s00299-007-0344-5
2. Abreu, I.N.D., A.C.H.F. Sawaya, M.N. Eberlin and P. Mazzafera, 2005. Production of pilocarpine in callus of jaborandi (pilocarpus microphyllus stapf). Vitro Cellular Dev. Biology-Plant, 41: 806-811. DOI: 10.1079/IVP2005711
3. Akalezi, C.O., S. Liu, Q.S. Li, J.T. Yu and J.J. Zhong, 1999. Combined effects of initial sucrose concentration and inoculum size on cell growth and ginseng saponin production by suspension cultures of panax ginseng. Process Biochem., 34 639-642. DOI: 10.1016/S0032-9592(98)00132-0
4. Al-Juboory, K.H., R.M. Skirvin and D.J. William, 1998. Callus induction and adventitious shoot regeneration of gardenia (gardenia jasminoisides ellis) leaf explants. Sci. Hortic., 72: 171-178. DOI: 10.1016/S0304-4238(97)00060-5
5. Andreazza, N.L., I.N. Abreu, A.C.H.F. Sawaya, M.N. Eberlin and P. Mazzafera, 2008. Production of imidazole alkaloids in cell cultures of jaborandi as affected by the medium ph. Biotechnol. Lett. 31: 607-614. DOI: 10.1007/s10529-008-9895-y
6. Antonigni, F., S. Zheng, C. Pagnucco, R. Baraldi and F. Poli et al., 2007. Induction of flavonoid production by UV-B radiation in Passiflora quagrangularis passiflora callus cultures. Fitoterapia 78: 345-352. DOI: 10.1016/j.fitote.2007.02.001
7. Arias-Castro, C., A.H. Scragg and M.A. Rodriguez-Mendiola, 1993. The effect of cultural conditions on the accumulation of formononetin by suspension cultures of glycyrrhiza glabra. Plant Cell. Tiss. Org., 34: 63-70. DOI: 10.1007/BF00048464
8. Arvy, M.P., N. Imbault, F. Naudascher, M. Thiersault and P. Doireau, 1994. 2, 4-D and alkaloid accumulation in periwinkle cell suspensions. Biochemistry, 76: 410-416. DOI: 10.1016/0300-9084(94)90117-1
9. Bakhshi, D. and O. Arakawa, 2006. Induction of phenolic compounds biosynthesis with light irradiation in the flesh of red and yellow apples. J. Appl. Hortic., 8: 101-104. http://www.horticultureresearch.net/Induction%20of%20phenolic%20compounds%20biosynthesis%20with%20light%20irradiation%20%2017-20.pdf
10. Banerjee, S., M. Zehra and S. Kumar, 1999. In vitro multiplication of centella asiatica, a medicinal herb from leaf explants. Curr. Sci. India 76: 147-148. http://www.bioone.org/doi/abs/10.1007/s11627-007-9040-y
11. Baskaran, P., B.R. Rajeswari and N. Jayabalan, 2006. Development of an in vitro regeneration system in sorghum [Sorghum bicolor (L). Moench] using root transverse Thin Cell Layers (tTCLs). Turk. J. Bot., 30: 1-9. http://journals.tubitak.gov.tr/botany/issues/bot-06-30-1/bot-30-1-1-0502-3.pdf
12. Brinkhaus, B., M. Lindner, D. Schuppanand E.G. Hahn, 2000. Chemical, pharmacological and clinical profile of the East Asian medical plant centella asiatica. Phytomedicine, 7: 427-448.
13. Cenkci, S., M. Kargioglu, S. Dayan and M. Konuk, 2008. In vitro propagation of an endangered plant species, thermopsis turcica (fabaceae). Biologia, 63: 652-657. DOI: 10.2478/s11756-008-0125-9
14. Chakraborty, A. and S. Chattopadhyay, 2008. Stimulation of menthol production in menthe piperita cell culture. Vitro Cellular Dev. Biology-Plant, 44: 518-524. DOI: 10.1007/s11627-008-9145-y
15. Cheng, C.L. and M.W.L. Koo, 2000. Effects of centella asiatica on ethanol induced gastric mucosal lesions in rats. Life Sci., 67: 2647-2653. DOI: 10.1016/S0024-3205(00)00848-1
16. Cho, H.Y., S.Y. Son, H.S. Rhee, S.Y.H. Yoon and C.W.T. Lee-Parsons et al., 2008. Synergistic effects of sequential treatment with methyl jasmonate, salicylic acid and yeast extract on benzophenanthridine alkaloid accumulation and protein expression in eschscholtzia californica suspension cultures. J. Biotechnol., 135: 117-122. DOI: 10.1016/j.jbiotec.2008.02.020
17. Cook, N.C. and S. Samman, 1996. Flavonoidschemistry, metabolism, cardioprotective effects and dietary sources. Nutr. Biochem., 7: 66-76. DOI: 10.1016/S0955-2863(95)00168-9
18. Crozier, A., E. Jensen, M.E.J. Lean and M.S. McDonald, 1997. Quantitative analysis of flavonoids by reversed-phase high-performance liquid chromatography. J. Chromatogr. A., 761: 315-321. DOI: 10.1016/S0021-9673(96)00826-6
19. Fu, C.X., D.X. Zhao, Y. Huang and F.S. Ma, 2005. Cellular aggregate size as the critical factor for flavonoid production by suspension cultures of saussurea medusa. Biotechnol. Lett., 27: 91-95. DOI: 10.1007/s10529-004-6934-1
20. Gaba, V.P. 2005. Plant Growth Regulators in Plant Tissue Culture and Development. In: Plant Development and Biotechnology. Trigiano, R.N. and D.J. Gray (Eds.). CRC Press, Washington DC., pp: 87-99.
21. Gamborg, O.L., R.A. Miller and K. Ojima, 1968. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell. Res. 50: 151-158. DOI: 10.1016/0014-4827(68)90403-5
22. Gopi, C. and T.M. Vatsala, 2006. In vitro studies on effects of plant growth regulators on callus and suspension culture biomass yield from gymnema sylvestre. R. Br. Afr. J. Biotechnol., 2: 1215-1219. http://ajol.info/index.php/ajb/article/viewFile/60847/49059
23. Hasan, S.M.R., M. Jamila, M.M. Majumder, R. Akter, M.M. Hossain and M.E.H. Mazumder, 2009. Analgesic and antioxidant activity of the hydromethanolic extract of Mikania scandens (L.) willd leaves. Am. J. Pharmacol. Toxicol., 4: 1-7. DOI: 10.3844/ajptsp.2009.1.7
24. Hutchinson, M.J., J.M. Tsujita and P.K. Saxena, 1994. Callus induction and plant regeneration from mature zygotic embryos of a tetraploid alstroemeria (A. pelegrina x A. cinapsitta). Plant Cell Reports, 14: 184-187. DOI: 10.1007/BF00233787
25. Jacinda, J.T., R. Meyer and I.A. Dubery, 2008. Characterisation of two phenotypes of centella asiatica in Southern Africa through the composition of four triterpenoids in callus, cell suspensions and leaves. Plant Cell. Tiss. Org., 94: 91-99. DOI: 10.1007/s11240-008-9391-z
26. Karami, O. 2008. Induction of embryogenic callus and plant regeneration in carnation (Dianthus caryophyllus L.). OnLine J. Bio. Sci., 8: 68-72. DOI: 10.3844/ojbsci.2008.68.72
27. Lee, S.H., N. Ahsan, K.W. Lee, D.G. Lee and I. Alam et al., 2007. Re-evaluation of mature seed-derived callusing and regeneration potential of nine orchardgrass (dactylis golomerata L.) cultivars. Plant Tiss. Cult. Biotechnol., 17: 193-207. DOI: 10.3329/ptcb.v17i2.3240
28. Lentini, A., C. Forni, B. Provenzano and S. Beninati, 2007. Enhancement of transglutaminase activity and polyamine depletion in B16-F10 melanoma cells by flavonoids naringenin and hesperitin correlate to reduction of the in vivo metastatic potential. Amino. Acids., 32: 95-100. DOI: 10.1007/s00726-006-0304-3
29. Liang, L.F., C.L. Keng and B.P. Lim, 2006. Selection of cell lines for the production of rosmarinic acid from cell suspension cultures of Orthosiphon stamineus benth. Vitro Cellular Dev. Biology-Plant, 42: 538-542. DOI: 10.1079/IVP2006813
30. Marinova, D., F. Ribarova and M. Atanassova, 2005. Total phenolics and total flavonoids in Bulgarian fruits and vegetables. J. Univ. Chem. Technol. Metall., 40: 255-260. http://www.uctm.edu/journal/j2005-3/Marinova.pdf
31. Martin, K.P., 2004. Plant regeneration through somatic embryogenesis in medicinally important centella asiatica L. Vitro Cellular Dev. Biology-Plant, 40: 586-591. http://www.jstor.org/pss/4293798
32. Mato, L., J. Wattanathorn, S. Muchimapura, T. Tongun and N. Piyawatkul et al., 2009. Centella asiatica Improves Physical Performance and Health-Related Quality of Life in Healthy Elderly Volunteer. Evidence-Based Complete Alternation Medicine.
33. Maurmann, N., C.M.B.D. Carvalho, A.L. Silva, A.G. Fett-neto and G.L.V. Poser et al., 2006. Valepotriates accumulation in callus, suspended cells and untransformed root cultures of valeriana glechomifolia. Vitro Cellular Dev. Biology-Plant, 42: 50-53. DOI: 10.1079/IVP2005725
34. Miguel-Chavez, R.S., M. Soto-Hernandez, A.C. Romos-Valdivia, G. Kite and M. Martinez-Vazquez et al., 2003. Production of alkaloids by in vitro culture of erythrina americana miller. Biotechnol. Lett., 25: 1055-1059. DOI: 10.1023/A:1024142531691
35. Mishiba, K.I., T. Okamoto and M. Mii, 2001. Increasing ploidy level in cell suspension cultures of doritaenopsis by exogenous application of 2,4-dichlorophenoxyacetic acid. Physiol. Plantarum., 112: 142-148. DOI: 10.1034/j.1399-3054.2001.1120119.x
36. Mohandas, R.K.G., R.S. Muddanna and R.S. Gurumadhva, 2009. Enhancement of amygdaloid neuronal dendritic arborization by fresh leaf juice of centella asiatica (Linn) during growth spurt period in rats. Evidence-based Compl. Alt. Med., 6: 203-210. DOI: 10.1093/ecam/nem079
37. Mohapatra, H., D.P. Barik and S.P. Rath, 2008. In vitro regeneration of medicinal plant centella asiatica. Biol. Plantarum., 52: 339-342. DOI: 10.1007/s10535-008-0069-5
38. Mucimapura, S., J. Wattanathorn, S. Thongrong, K. Chaisiwamongkol and B. Sripanidkulchai, 2010. Morus alba enhanced functional recovery after sciatic nerve crush injury. Am. J. Agri. Bio. Sci., 5: 294-300. DOI: 10.3844/ajabssp.2010.294.300
39. Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bio assays with tobacco tissue culture. Physiol. Plantarum., 15: 473-497. DOI: 10.1111/j.1399-3054.1962.tb08052.x
40. Murthy, B.N.S., S.J. Murch and P.K. Saxena, 1998. Thidiazuron: A potent regulator of in vitro plant morphogenesis. Vitro Cell Dev. Biology-Plant, 34: 267-275. http://cat.inist.fr/?aModele=afficheN©cpsidt=1603421
41. Nath, S. and A.K. Buragohain, 2005. Establishment of callus and cell suspension cultures of centella asiatica. Biol. Plantarum., 49: 411-413. DOI: 10.1007/s10535-005-0017-6
42. Nay, M.M.T. Saw, H. Riedel, O. Kütük, K. Ravichandran and I. Smetanska, 2010. Effect of elicitors and precursors on the synthesis of anthocyanin in grape vitis vinifera cell cultures. Energy Res. J., 1: 189-192. DOI: 10.3844/ERJSP.2010.189.192
43. Nontaswatsri, C., S. Ruamrungsri and S. Fukai, 2008. Callus induction and plant regeneration of dianthus chinensis L. and dianthus barbatus L. via anther culture. Acta Hort., 788: 109-114. http://www.actahort.org/members/showpdf?booknrarnr=788_12
44. Pellegrineschi, A., R.M. Brito, S. McLean and D. Hoisington, 2004. Effect of 2, 4-dichlorophenoxyacetic acid and NaCl on the establishment of callus and plant regeneration in durum and bread wheat. Plant Cell. Tiss., Org., 77: 245-250. DOI: 10.1023/B:TICU.0000018389.99656.d8
45. Peterson, J. and J. Dwyer, 1998. Flavonoids: Dietary occurrence and biochemical activity. Nutr. Res., 18: 1995-2018. DOI: 10.1016/S0271-5317(98)00169-9
46. Salma, U., M.S.M. Rahman, S. Islam, N. Haque and T.A. Jubair et al., 2008. The influence of different concentration and combination on callus induction and regeneration of rauwolfia serpentine L. Benth. Pak. J. Biol. Sci., 11: 1638-1641. DOI: 10.3923/PJBS.2008.1638.1641
47. Shirani, S., M. Sariah, W. Zakaria and M. Maziah, 2010. Scalp induction rate responses to cytokinins on proliferating shoot-tips of banana cultivars (Musa spp.). Am. J. Agri. Bio. Sci., 5: 128-134. DOI: 10.3844/ajabssp.2010.128.134
48. Syamsudin, R.M. Dewi and Kusmardi, 2009. Immunomodulatory and In vivo antiplasmodial activities of propolis extracts. Am. J. Pharmacol. Toxicol., 4: 75-79. DOI: 10.3844/ajptsp.2009.75.79
49. Tohidi-Moghadam, H.R., A.H. Shirani-Rad, G. Nour-Mohammadi, D. Habibi and M. Mashhadi-Akbar-Boojar, 2009. Effect of super absorbent application on antioxidant enzyme activities in canola (Brassica napus L.) cultivars under water stress conditions. Am. J. Agri. Bio. Sci., 4: 215-223. DOI: 10.3844/ajabssp.2009.215.223
50. Whitmer, S., R. Verpoorte and C. Canel, 1998. Influence of auxins on alkaloid accumulation by a transgenic cell line of catharanthus roseus. Plant Cell. Tiss. Org., 53: 135-141. DOI: 10.1023/A:1006019620897
51. Wu, C.H., Y.H. Dewir, E.J. Hahn and K.Y. Paek, 2006. Optimization of culturing conditions for the production of biomass and phenolics from adventitious roots of echinacea angustifolia. J. Plant Biol., 49: 193-199. DOI: 10.1007/BF03030532
52. Wu, S.X., Y.G. Zuand M. Wu, 2003. High yield production of salidroside in the suspension culture of rhodiola sachalinensis. J. Biotechnol., 106: 33-43. DOI: 10.1016/j.jbiotec.2003.07.009
53. Xu, L., U. Najeed, R. Raziuddin, W.Q. Shen and J.Y. Shou et al., 2009. Development of an efficient tissue culture protocol for callus formation and plant regeneration of wetland species Juncus effuses L. Vitro Cellular Dev. Biology-Plant, 45: 610-618. DOI: 10.1007/s11627-009-9228-4
54. Yan, M.M., C. Xu, C.H. Kim, Y.C. Um and A.A. Bah et al., 2009. Effects of explant type, culture media and growth regulators on callus induction and plant regeneration of Chinese jiaotou allium chinense. Sci. Hortic., 123: 124-128. DOI: 10.1016/j.scienta.2009.07.021
55. Yang, J., Z.C. Gong and X. Tan, 2008. Induction of callus and extraction of alkaloid from Yi Mu Cao (leonurus heterophylus Sw.) culture. Afr. J. Biotechnol. 7: 1157-1162. http://ajol.info/index.php/ajb/article/viewFile/58637/46971
56. Yang, Z.S., G.D. Chen, Y.X. Li and J. Chen, 2009. Characterization of callus formation in leaf of Euphorbia helioscopia. Afr. J. Plant Sci., 3: 122-126. http://www.academicjournals.org/AJPS/PDF/Pdf2009/Jun/Yang%20et%20al.pdf
57. Zainol, M.K., A. Abd-Hamid, S. Yusof and R. Muse, 2003. Antioxidative activity and total phenolic compounds of leaf, root and petiole of four accessions of centella asiatica L. Urban. Food Chem., 81: 575-581. DOI: 10.1016/S0308-8146(02)00498-3
58. Zhao, D.X., J.M. Xing, M.Y. Li, D.P. Lu and Q, 2001. Optimization of growth and jaceosidin production in callus and cell suspension cultures of saussurea medusa. Plant Cell. Tiss. Org., 67: 227-234. DOI: 10.1023/A:1012770623153
59. Zhao, L., S. Liu and S. Song, 2010. Optimization of callus induction and plant regeneration from germinating seeds of sweet sorghum (sorghum bicolor mench). Afr. J. Biotechnol., 9: 2367-2374. http://eprints.icrisat.ac.in/572/1/AfricanJBiotechnol ogy9_16_2367_2374_2010.pdf