Transfection of truncated bone morphogenetic protein receptor-II into oral squamous cell carcinoma cell line Tca8113 and inhibitory effect on proliferation and inductive effect on apoptosis

Journal of Oral Pathology and Medicine

Volumn 40, Issue 6, 2011, Pages 490-496

  Fu, Sheng ^a   Lv, Hong Bing ^b   Liu, Yuan ^c   Zhao, Yu ^c   He, Li Sheng ^c   Jin, Yan ^c  

^a FUZHOU GENERAL HOSPITAL   (China)

^b FUJIAN MEDICAL UNIVERSITY   (China)

^c FOURTH MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

Apoptosis; Bone morphogenetic protein receptor; Oral squamous cell carcinoma; Proliferation; Transfection

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; BONE MORPHOGENETIC PROTEIN RECEPTOR 2; BROXURIDINE; CYCLIN D1; CYCLIN DEPENDENT KINASE 4; MESSENGER RNA; PROTEIN P27; PROTEIN P57;

APOPTOSIS; ARTICLE; CANCER CELL CULTURE; CANCER INHIBITION; CELL ASSAY; CELL CYCLE ARREST; CELL CYCLE G0 PHASE; CELL CYCLE G1 PHASE; CELL PROLIFERATION; CELL TRANSFORMATION; CELL VIABILITY; CONTROLLED STUDY; DOWN REGULATION; GENE OVEREXPRESSION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; MITOSIS INHIBITION; MOUTH CARCINOMA; NICK END LABELING; PHENOTYPE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DETERMINATION; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SQUAMOUS CELL CARCINOMA; UPREGULATION;

ANALYSIS OF VARIANCE; APOPTOSIS; BONE MORPHOGENETIC PROTEIN RECEPTORS, TYPE II; BONE MORPHOGENETIC PROTEINS; CARCINOMA, SQUAMOUS CELL; CELL CYCLE; CELL LINE, TUMOR; CELL PROLIFERATION; CYCLIN D1; CYCLIN-DEPENDENT KINASE 4; CYCLIN-DEPENDENT KINASE INHIBITOR P57; DOWN-REGULATION; G0 PHASE; HUMANS; IN SITU NICK-END LABELING; MOUTH NEOPLASMS; MUTATION; PROLIFERATING CELL NUCLEAR ANTIGEN; SIGNAL TRANSDUCTION; TRANSFECTION;

EID: 79960024454     PISSN: 09042512     EISSN: 16000714     Source Type: Journal    
DOI: 10.1111/j.1600-0714.2010.00988.x     Document Type: Article

References (25)

1. Yang LJ, Jin Y. Immunohistochemical observations on BMP in normal and abnormal conditions. Clin Orthop Relat Res 1990; 257: 249-56.
2. Wozeny JM. The bone morphogenetic protein family and osteogenesis. Mol Reprod Dev 1992; 32: 160-7.
3. Kingsley DM. The TGFβ superfamily: new members, new receptors, and new genetic tests of function in different organisms. Genes Dev 1994; 8: 133-46.
4. Hatakeyama S, Satoh M, Yoshimura N, Otsu T. Immunocytochemical localization of bone morphogenetic proteins (BMPs) in salivary pleomorphic adenoma. J Oral Pathol Med 1994; 23: 232-6.
5. Raida M, Sarbia M, Clement JH, Adam S, Gabbert HE, Höffken K. Expression, regulation and clinical significance of bone morphogenetic protein 6 in esophageal squamous-cell carcinoma. Int J Cancer 1999; 83: 38-44.
6. Hatakeyama S, Gao YH, Ohara-Nemoto Y, Kataoka H, Satoh M. Expression of bone morphogenetic proteins of human neoplastic epithelial cells. Biochem Mol Biol Int 1997; 42: 497-505.
7. Jin Y, Tipoe GL, Liong EC, Lau TY, Fung PC, Leung KM. Overexpression of BMP-2/4, -5 and BMPR-IA associated with malignancy of oral epithelium. Oral Oncol 2001; 37: 225-33.
8. Suzuki A, Thies RS, Yamaji N, et al. A truncated bone morphogenetic protein receptor affects dorsal-ventral patterning in the early Xenopus embryo. Proc Natl Acad Sci USA 1994; 91: 10255-9.
9. Lu H, Jin Y, Tipoe GL. Alteration of BMP-2, 3, 4, 5 mRNA during the pathogenesis of cleft palate in BALB/c mice. Arch Oral Biol 2000; 45: 133-40.
10. Oberholzer M, Ostreicher M, Christen H, Brühlmann M. Methods in quantitative image analysis. Histochem Cell Biol 1996; 105: 333-55.
11. Sneddon JB, Zhen HH, Montgomery K, et al. Bone morphogenetic protein antagonist gremlin 1 is widely expressed by cancerassociated stromal cells and can promote tumor cell proliferation. Proc Natl Acad Sci U S A 2006; 103: 14842-7.
12. Grijelmo C, Rodrigue C, Svrcek M, et al. Proinvasive activity of BMP-7 through SMAD4/src-independent and ERK/Rac/JNKdependent signaling pathways in colon cancer cells. Cell Signal 2007; 19: 1722-32.
13. Kim IY, Lee DH, Lee DK, et al. Restoration of bone morphogenetic protein receptor type II expression leads to a decreased rate of tumor growth in bladder transitional cell carcinoma cell line TSU-Pr1. Cancer Res 2004; 64: 7355-60.
14. Kim IY, Lee DH, Lee DK, et al. Decreased expression of bone morphogenetic protein (BMP) receptor type II correlates with insensitivity to BMP-6 in human renal cell carcinoma cells. Clin Cancer Res 2003; 9: 6046-51.
15. Malumbres M, Barbacid M. Cell cycle, CDKs and cancer: a changing paradigm. Nature 2009; 9: 153-66.
16. Landis MW, Pawlyk BS, Li T, Sicinski P, Hinds PW. Cyclin D1-dependent kinase activity in murine development and mammary tumorigenesis. Cancer Cell 2006; 9: 13-22.
17. Besson A, Hwang HC, Cicero S, et al. Discovery of an oncogenic activity in p27Kip1 that causes stem cell expansion and a multiple tumor phenotype. Genes Dev 2007; 21: 1731-46.
18. Walkley CR, Fero ML, Chien WM, Purton LE, McArthur GA. Negative cell-cycle regulators cooperatively control self-renewal and differentiation of haematopoietic stem cells. Nat Cell Biol 2005; 7: 172-8.
19. Kippin TE, Martens DJ, van der Kooy D. p21 loss compromises the relative quiescence of forebrain stem cell proliferation leading to exhaustion of their proliferation capacity. Genes Dev 2005; 19: 756-67.
20. Hjertner O, Hjorth-Hansen H, Börset M, Seidel C, Waage A, Sundan A. Bone morphogenetic protein-4 inhibits proliferation and induces apoptosis of multiple myeloma cells. Blood 2001; 97: 516-22.
21. Glozak MA, Rogers MB. Retinoic acid- and bone morphogenetic protein 4-induced apoptosis in P19 embryonal carcinoma cells requires p27. Exp Cell Res 2001; 268: 128-38.
22. Hallahan AR, Pritchard JI, Chandraratna RA, et al. BMP-2 mediates retinoid-induced apoptosis in medulloblastoma cells through a paracrine effect. Nat Med 2003; 9: 1033-8.
23. Raida M, Clement JH, Ameri K, Han C, Leek RD, Harris AL. Expression of bone morphogenetic protein 2 in breast cancer cells inhibits hypoxic cell death. Int J Oncol 2005; 26: 1465-70.
24. Deng H, Ravikumar TS, Yang WL. Bone morphogenetic protein-4 inhibits heat-induced apoptosis by modulating MAPK pathways in human colon cancer HCT116 cells. Cancer Lett 2007; 256: 207-17.
25. Wei A, Brisby H, Chung SA, Diwan AD. Bone morphogenetic protein-7 protects human intervertebral disc cells in vitro from apoptosis. Spine J 2008; 8: 466-74.