Exposure to 50Hz electromagnetic field raises the levels of the anti-apoptotic protein BAG3 in melanoma cells

Journal of Cellular Physiology

Volumn 226, Issue 11, 2011, Pages 2901-2907

  Basile, Anna ^a,b   Zeppa, Rosario ^a   Pasquino, Nicola ^c   Arra, Claudio ^d   Ammirante, Massimo ^a   Festa, Michelina ^a,b   Barbieri, Antonio ^d   Giudice, Aldo ^d   Pascale, Maria ^a,b   Turco, Maria Caterina ^a,b   Rosati, Alessandra ^a,b  

^a UNIVERSITY OF SALERNO   (Italy)

^b BIOUNIVERSA Srl   (Italy)

^c UNIVERSITY OF NAPLES FEDERICO II   (Italy)

^d NATIONAL CANCER INSTITUTE   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ANTI APOPTOTIC PROTEIN BAG3; PEPTIDES AND PROTEINS; SMALL INTERFERING RNA; UNCLASSIFIED DRUG; APOPTOSIS REGULATORY PROTEIN; BAG3 PROTEIN, HUMAN; HEAT SHOCK PROTEIN 70; HEAT SHOCK PROTEIN 72; SIGNAL TRANSDUCING ADAPTOR PROTEIN;

ANIMAL EXPERIMENT; ANIMAL MODEL; APOPTOSIS; ARTICLE; CELL MEMBRANE DEPOLARIZATION; CELL SURVIVAL; CONTROLLED STUDY; ELECTROMAGNETIC FIELD; FEMALE; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IN VITRO STUDY; MELANOMA; MITOCHONDRIAL MEMBRANE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; STRESS; TUMOR CELL LINE; TUMOR XENOGRAFT; ANIMAL; METABOLISM; NUDE MOUSE; PHYSIOLOGICAL STRESS; SKIN TUMOR;

ADAPTOR PROTEINS, SIGNAL TRANSDUCING; ANIMALS; APOPTOSIS; APOPTOSIS REGULATORY PROTEINS; CELL LINE, TUMOR; CELL SURVIVAL; ELECTROMAGNETIC FIELDS; FEMALE; HSP70 HEAT-SHOCK PROTEINS; HSP72 HEAT-SHOCK PROTEINS; HUMANS; MELANOMA; MICE; MICE, NUDE; SKIN NEOPLASMS; STRESS, PHYSIOLOGICAL;

EID: 79959989528     PISSN: 00219541     EISSN: 10974652     Source Type: Journal    
DOI: 10.1002/jcp.22641     Document Type: Article

References (50)

1. Alfieri RR, Petronini PG, Bonelli MA, Desenzani S, Cavazzoni A, Borghetti AF, Wheeler KP. 2004. Roles of compatible osmolytes and heat shock protein 70 in the induction of tolerance to stresses in porcine endothelial cells. J Physiol 16: 757-767.
2. Alfieri RR, Bonelli MA, Pedrazzi G, Desenzani S, Ghillani M, Fumarola C, Ghibelli L, Borghetti AF, Petronini PG. 2006. Increased levels of inducible HSP70 in cells exposed to electromagnetic fields. Radiat Res 165: 95-104.
3. Ammirante M, Rosati A, Arra C, Basile A, Falco A, Festa M, Pascale M, d'Avenia M, Marzullo L, Belisario MA, De Marco M, Barbieri A, Giudice A, Chiappetta G, Vuttariello E, Monaco M, Bonelli P, Salvatore G, Di Benedetto M, Deshmane SL, Khalili K, Turco MC, Leone A. 2010. IKK{gamma} protein is a target of BAG3 regulatory activity in human tumor growth. Proc Natl Acad Sci USA 107: 7497-7502.
4. Benassi B, Zupi G, Biroccio A. 2007. Gamma-glutamylcysteine synthetase mediates the c-Myc-dependent response to antineoplastic agents in melanoma cells. Mol Pharmacol 72: 1015-1023.
5. Bernard N, Alberdi AJ, Tanguy ML, Brugere H, Helissey P, Hubert C, Gendrey N, Guillosson JJ, Nafziger J. 2008. Assessing the potential leukemogenic effects of 50Hz magnetic fields and their harmonics using an animal leukemia model. J Radiat Res (Tokyo) 49: 565-577.
6. Bernardini C, Zannoni A, Turba ME, Bacci ML, Forni M, Mesirca P, Remondini D, Castellani G, Bersani F. 2007. Effects of 50Hz sinusoidal magnetic fields on Hsp27, Hsp70, Hsp90 expression in porcine aortic endothelial cells (PAEC). Bioelectromagnetics 28: 231-237.
7. Blank M, Goodman R. 2009. Electromagnetic fields stress living cells. Pathophysiology 16: 71-78.
8. Bonelli P, Petrella A, Rosati A, Romano MF, Lerose R, Pagliuca MG, Amelio T, Festa M, Martire G, Venuta S, Turco MC, Leone A. 2004. BAG3 protein regulates stress-induced apoptosis in normal and neoplastic leukocytes. Leukemia 18: 358-360.
9. Carpenter DO. 2010. Electromagnetic fields and cancer: The cost of doing nothing. Rev Environ Health 25: 75-80.
10. Chiappetta G, Ammirante M, Basile A, Rosati A, Festa M, Monaco M, Vuttariello E, Pasquinelli R, Arra C, Zerilli M, Todaro M, Stassi G, Pezzullo L, Gentilella A, Tosco A, Pascale M, Marzullo L, Belisario MA, Turco MC, Leone A. 2007. The antiapoptotic protein BAG3 is expressed in thyroid carcinomas and modulates apoptosis mediated by tumor necrosis factor-related apoptosis-inducing ligand. J Clin Endocrinol Metab 92: 1159-1163.
11. Crompton M. 2000. Mitochondrial intermembrane junctional complexes and their role in cell death. J Physiol 15: 11-21.
12. de Bruyn M, Rybczynska AA, Wei Y, Schwenkert M, Fey GH, Dierckx RA, van Waarde A, Helfrich W, Bremer E. 2010. Melanoma-associated Chondroitin Sulfate Proteoglycan (MCSP)-targeted delivery of soluble TRAIL potently inhibits melanoma outgrowth in vitro and in vivo. Mol Cancer 23: 301.
13. De Nicola M, Cordisco S, Cerella C, Albertini MC, D'Alessio M, Accorsi A, Bergamaschi A, Magrini A, Ghibelli L. 2006. Magnetic fields protect from apoptosis via redox alteration. Ann NY Acad Sci 1090: 59-568.
14. Deans JK, Powell AD, Jefferys JG. 2007. Sensitivity of coherent oscillations in rat hippocampus to AC electric fields. J Physiol 1: 555-565.
15. Del Re B, Marcantonio P, Bersani F, Giorgi G. 2009. Extremely low frequency magnetic field exposure affects DnaK and GroEL expression in E. coli cells with impaired heat shock response. Gen Physiol Biophys 28: 420-424.
16. Di Loreto S, Falone S, Caracciolo V, Sebastiani P, D'Alessandro A, Mirabilio A, Zimmitti V, Amicarelli F. 2009. Fifty hertz extremely low-frequency magnetic field exposure elicits redox and trophic response in rat-cortical neurons. J Cell Physiol 219: 334-343.
17. Flipo D, Fournier M, Benquet C, Roux P, Le Boulaire C, Pinsky C, LaBella FS, Krzystyniak K. 1998. Increased apoptosis, changes in intracellular Ca2+, and functional alterations in lymphocytes and macrophages after in vitro exposure to static magnetic field. J Toxicol Environ Health A 54: 63-76.
18. Focke F, Schuermann D, Kuster N, Schär P. 2010. DNA fragmentation in human fibroblasts under extremely low frequency electromagnetic field exposure. Mutat Res 683: 74-83.
19. Frahm J, Mattsson MO, Simkó M. 2010. Exposure to ELF magnetic fields modulate redox related protein expression in mouse macrophages. Toxicol Lett 192: 330-336.
20. Franceschelli S, Rosati A, Lerose R, De Nicola S, Turco MC, Pascale M. 2008. Bag3 gene expression is regulated by heat shock factor 1. J Cell Physiol 215: 575-577.
21. Garip AI, Akan Z. 2010. Effect of ELF-EMF on number of apoptotic cells; correlation with reactive oxygen species and HSP. Acta Biol Hung 61: 158-167.
22. Girgert R, Schimming H, Körner W, Gründker C, Hanf V. 2005. Induction of tamoxifen resistance in breast cancer cells by ELF electromagnetic fields. Biochem Biophys Res Commun 336: 1144-1149.
23. Hilmi C, Larribere L, Giuliano S, Bille K, Ortonne JP, Ballotti R, Bertolotto C. 2008. IGF1 promotes resistance to apoptosis in melanoma cells through an increased expression of BCL2, BCL-X(L), and survivin. J Invest Dermatol 128: 1499-1505.
24. Ilangovan G, Venkatakrishnan CD, Bratasz A, Osinbowale S, Cardounel AJ, Zweier JL, Kuppusamy P. 2006. Heat shock-induced attenuation of hydroxyl radical generation and mitochondrial aconitase activity in cardiac H9c2 cells. Am J Physiol Cell Physiol 290: C313-2.
25. Juutilainen J. 2008. Do electromagnetic fields enhance the effects of environmental carcinogens? Radiat Prot Dosimetry 132: 228-231.
26. Kakkar P, Singh BK. 2007. Mitochondria: A hub of redox activities and cellular distress control. Mol Cell Biochem 305: 235-253.
27. Kim YW, Kim HS, Lee JS, Kim YJ, Lee SK, Seo JN, Jung KC, Kim N, Gimm YM. 2009. Effects of 60Hz 14 microT magnetic field on the apoptosis of testicular germ cell in mice. Bioelectromagnetics 30: 66-72.
28. Kozloski GA, Carraway CA, Carraway KL. 2010. Mechanistic and signaling analysis of Muc4-ErbB2 signaling molecule: New insights into the mechanism of ligand-independent ErbB2 activity. J Cell Physiol 224: 649-657.
29. Liu J, Kam KW, Borchert GH, Kravtsov GM, Ballard HJ, Wong TM. 2006. Further study on the role of HSP70 on Ca2+ homeostasis in rat ventricular myocytes subjected to simulated ischemia. Am J Physiol Cell Physiol 290: C583-C591.
30. Liu P, Xu B, Li J, Lu H. 2009. BAG3 gene silencing sensitizes leukemic cells to Bortezomib-induced apoptosis. FEBS Lett 583: 401-406.
31. Miyabara EH, Martin JL, Griffin TM, Moriscot AS, Mestril R. 2006. Overexpression of inducible 70-kDa heat shock protein in mouse attenuates skeletal muscle damage induced by cryolesioning. Am J Physiol Cell Physiol 290: C1128-C1138.
32. Morabito C, Rovetta F, Bizzarri M, Mazzoleni G, Fanò G, Mariggiò MA. 2010. Modulation of redox status and calcium handling by extremely low frequency electromagnetic fields in C2C12 muscle cells: A real-time, single-cell approach. Free Radic Biol Med 48: 579-589.
33. Morimoto RI. 1998. Regulation of the heat shock transcriptional response: Cross talk between a family of heat shock factors, molecular chaperones, and negative regulators. Genes Dev 12: 3788-3796.
34. Narita K, Hanakawa K, Kasahara T, Hisamitsu T, Asano K. 1997. Induction of apoptotic cell death in human leukemic cell line, HL-60, by extremely low frequency electric magnetic fields: Analysis of the possible mechanisms in vitro. In Vivo 11: 329-335.
35. Nollen EA, Morimoto RI. 2002. Chaperoning signaling pathways: molecular chaperones as stress-sensing 'heat shock' proteins. J Cell Sci 115: 2809-2816.
36. Oda T, Koike T. 2004. Magnetic field exposure saves rat cerebellar granule neurons from apoptosis in vitro. Neurosci Lett 365: 83-86.
37. Pilger A, Ivancsits S, Diem E, Steffens M, Kolb HA, Rüdiger HW. 2004. No effects of intermittent 50Hz EMF on cytoplasmic free calcium and on the mitochondrial membrane potential in human diploid fibroblasts. Radiat Environ Biophys 43: 203-207.
38. Rafiee P, Theriot ME, Nelson VM, Heidemann J, Kanaa Y, Horowitz SA, Rogaczewski A, Johnson CP, Ali I, Shaker R, Binion DG. 2006. Human esophageal microvascular endothelial cells respond to acidic pH stress by PI3K/AKT and p38 MAPK-regulated induction of Hsp70 and Hsp27. Am J Physiol Cell Physiol 291: C931-C945.
39. Romano MF, Festa M, Pagliuca G, Lerose R, Bisogni R, Chiurazzi F, Storti G, Volpe S, Venuta S, Turco MC, Leone A. 2003a. BAG3 protein controls B-chronic lymphocytic leukaemia cell apoptosis. Cell Death Differ 10: 383-385.
40. Romano MF, Festa M, Petrella A, Rosati A, Pascale M, Bisogni R, Poggi V, Kohn EC, Venuta S, Turco MC, Leone A. 2003b. BAG3 protein regulates cell survival in childhood acute lymphoblastic leukemia cells. Cancer Biol Ther 2: 508-510.
41. Röösli M, Lörtscher M, Egger M, Pfluger D, Schreier N, Lörtscher E, Locher P, Spoerri A, Minder C. 2007. Leukaemia, brain tumours and exposure to extremely low frequency magnetic fields: Cohort study of Swiss railway employees. Occup Environ Med 64: 553-559.
42. Rosati A, Ammirante M, Gentilella A, Basile A, Festa M, Pascale M, Marzullo L, Belisario MA, Tosco A, Franceschelli S, Moltedo O, Pagliuca G, Lerose R, Turco MC. 2007. Apoptosis inhibition in cancer cells: A novel molecular pathway that involves BAG3 protein. Int J Biochem Cell Biol 39: 1337-1342.
43. Ruiz Gómez MJ, De la Peña L, Pastor JM, Martínez Morillo M, Gil L. 2001. 25Hz electromagnetic field exposure has no effect on cell cycle distribution and apoptosis in U-937 and HCA-2/1cch cells. Bioelectrochemistry 53: 137-140.
44. Tofani S, Barone D, Cintorino M, de Santi MM, Ferrara A, Orlassino R, Ossola P, Peroglio F, Rolfo K, Ronchetto F. 2001. Static and ELF magnetic fields induce tumor growth inhibition and apoptosis. Bioelectromagnetics 22: 419-428.
45. Tokalov SV, Gutzeit HO. 2004. Weak electromagnetic fields (50Hz) elicit a stress response in human cells. Environ Res 94: 145-151.
46. Turrens JF. 2003. Mitochondrial formation of reactive oxygen species. J Physiol 15: 335-344.
47. Tynes T, Klaeboe L, Haldorsen T. 2003. Residential and occupational exposure to 50Hz magnetic fields and malignant melanoma: A population based study. Occup Environ Med 60: 343-347.
48. World Health Organization International Agency for Research on Cancer. 2002. Non-ionizing radiation. Part 1. Static and extremely low-frequency (ELF) electric and magnetic fields. Lyon-WHO (Monographs on the Evaluation of Carcinogenic Risks to Humans, Vol. 80).
49. World Health Organization. 2007. Extremely low frequency fields. Geneva: WHO.
50. Zhang XD, Gillespie SK, Hersey P. 2004. Staurosporine induces apoptosis of melanoma by both caspase-dependent and -independent apoptotic pathways. Mol Cancer Ther 3: 187-197.