Rapid separation of triacylglycerol positional isomers binding two saturated fatty acids using octacocyl silylation column

Journal of Oleo Science

Volumn 60, Issue 7, 2011, Pages 345-350

  Nagai, Toshiharu ^a   Gotoh, Naohiro ^b   Mizobe, Hoyo ^a   Yoshinaga, Kazuaki ^a   Kojima, Koichi ^a   Matsumoto, Yumiko ^b   Wada, Shun ^b  

^a Tsukishima Foods Industry Co   (Japan)

^b TOKYO UNIVERSITY OF MARINE SCIENCE AND TECHNOLOGY   (Japan)

Author keywords

Octacocyl silylation column; Saturated fatty acid; Separation; Triacylglycerol positional isomer; Unsaturated fatty acid

Indexed keywords

CHEMICAL IONIZATION; DOUBLE BONDS; MOBILE PHASE; POSITIONAL ISOMERS; RAPID SEPARATION; SILYLATIONS; TANDEM MASS SPECTROMETRY; TRIACYLGLYCEROLS;

ACETONE; ATMOSPHERIC IONIZATION; ATMOSPHERIC PRESSURE; CHEMICAL BONDS; COLUMN CHROMATOGRAPHY; GLYCEROL; HIGH PRESSURE LIQUID CHROMATOGRAPHY; IONIZATION OF LIQUIDS; ISOMERS; MASS SPECTROMETRY; PALMITIC ACID; UNSATURATED FATTY ACIDS;

SATURATED FATTY ACIDS;

FATTY ACID; OCTADECYLSILICON DIOXIDE; PALMITIC ACID DERIVATIVE; SILICON DIOXIDE; TRIACYLGLYCEROL;

ARTICLE; CHEMISTRY; ELECTROSPRAY MASS SPECTROMETRY; EVALUATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INSTRUMENTATION; ISOLATION AND PURIFICATION; ISOMERISM; METHODOLOGY; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; TANDEM MASS SPECTROMETRY; TIME;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; FATTY ACIDS; ISOMERISM; PALMITIC ACIDS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SILICON DIOXIDE; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TANDEM MASS SPECTROMETRY; TIME FACTORS; TRIGLYCERIDES;

EID: 79959837760     PISSN: 13458957     EISSN: 13473352     Source Type: Journal    
DOI: 10.5650/jos.60.345     Document Type: Article

References (18)

1. Murray, R. K.; Granner, D. K.; Rodwell, V. W. Harper's Illustrated Biochemistry. 27 ed., McGraw-Hill Companies. New York. pp.124 (2006).
2. Takano, S.; Kondoh, Y. Triglycerides analysis by combined argentation/non-aqueous reversed phase high performance liquid chromatography. J. Am. Oil Chem. Soc. 64, 380-383(1987).
3. Jensen, R. G.; Ferris, A. M.; Lammi-Keefe, C. J.; Henderson R. A. Lipids of bovine and human milks: A comparison. J. Dairy Sci. 73, 223-240 (1990).
4. Brown, J. B.; Orlans, B. M. The fatty acids of human milk fat. Arch. Biochem. 9, 201-219(1946).
5. Jensen, R. G. Lipids in human milk. Lipids 34, 1243-1271(1999).
6. Aoe, S.; Yamamura, J.; Matsuyama, H.; Hase, M.; Shio-ta, M.; Miura, S. The positional distribution of dioleoyl-palmitoyl glycerol influences lymph chylomicron transport, composition and size in rats. J. Nutr. 127, 1269-1273(1997).
7. Breckenridge, W. C.; Marai, L.; Kuksis, A. Triglyceride structure of human milk fat. Can. J. Biochem. 47, 761-769(1969).
8. Kuroda, I.; Nagai, T; Mizobe, H.; Yoshimura, N.; Gotoh, N.; Wada, S. HPLC separation of triacylglycerol positional isomers on a polymeric ODS column. Anal. Sci 24, 865-869(2008).
9. Gotoh, N.; Matsumoto, Y; Yuji, H.; Nagai, T; Mizobe, H.; Ichioka, K.; Kuroda, I.; Noguchi, N.; Wada, S. Characterization of non-endocapped polymeric ODS columnfor the separation of triacylglycerol positional isomers. J. Oleo Sci. 59, 71-79(2010).
10. Gotoh, N.; Matsumoto, Y.; Nagai, T.; Mizobe, H.; Otake, I.; Ichioka, K.; Kuroda, I.; Watanabe, H.; Noguchi, N.; Wada, S. Actual ratios of triacylglycerol positional isomers consisting of saturated and highly unsaturated fatty acids in fi shes and marine mammals. Food Chem. 127, 467-472(2011).
11. Owen R. F. Food Chemistry. 3 ed. Marcel Dekker Inc. New York. pp.231 (1996).
12. Sato, K.; Ueno, S. Polymorphism of fats and oils. in Bailey's Industrial Oil and Fat Products (Shahidi, F. ed.)Vol. 1, John Wiley & Sons Inc. New York. pp.77-120(2005).
13. Hunter, J. E. Studies on effect of dietary fatty acids as related to their position on triglycerides. Lipids 36, 655-668(2001).
14. Adolf, R. O. Recent advances in silver-ion HPLC utilizing acetonitrile in hexane as solvent: temperature effects on lipid elution orders/resolution. In Lipid Analysis and Lipidomics (Mossoba, M. M.; Kramer, J. K.G.; Brenna, J. T.; McDonald, R. E. ed.), AOCS Press. Champaign. pp.175-190 (2006).
15. Wada, S.; Koizumi, C.; Nonaka, J. Analysis of triglycerides of soybean oil by high performance liquid chromatography in combination with gas liquid chromatography. J. Jpn. Oil Chem. Soc. 26, 95-99 (1977).
16. Reich, E.; Schibli, A. High-Performance Thin-Layer Chromatography for the Analysis of Medical Plants. Thieme. New York. pp.31 (2006).
17. Wise, S. A.; Sander, L. C. Factors affecting the re-versed-phase liquid chromatographic separation of polycyclic aromatic hydrocarbon isomers. J. High Res. Chromatogr. Chromatogr. Commun. 8, 248-255 (1985).
18. Adolf, R. O. Separation of fatty acid methyl esters and triacylglycerols by Ag-HPLC: silver-ion and normal-phase contributions to retention. In New Techniques and Applications in Lipid Analysis (McDonald, R. E.; Mossoba, M. M. ed.), AOCS Press. Champaign. pp.256-265(1997).