Development of a PCR protocol for the detection of Escherichia coli O157:H7 and Salmonella spp. in surface water

Environmental Monitoring and Assessment

Volumn 177, Issue 1-4, 2011, Pages 493-503

  Bonetta, Silvia ^a   Borelli, Elena ^b   Bonetta, Sara ^a   Conio, Osvaldo ^b   Palumbo, Franca ^b   Carraro, Elisabetta ^a  

^a UNIVERSITY OF EASTERN PIEDMONT   (Italy)

^b Laboratori Iride Acqua Gas Srl   (Italy)

Author keywords

Escherichia coli O157:H7; PCR; Salmonella; Surface water

Indexed keywords

AMPLICONS; E. COLI; E.COLI O157:H7; ESCHERICHIA COLI O157:H7; HIGH SENSITIVITY; HUMAN HEALTH; INTIMIN; PATHOGENIC BACTERIUM; PATHOGENIC MICROORGANISMS; PCR; PHYSICAL ANALYSIS; POTENTIAL THREATS; ROUTINE ANALYSIS; SALMONELLA SPP; SHIGA-LIKE TOXINS; SPIKED WATER SAMPLES; VIRULENCE GENE; WATER-BORNE PATHOGENS;

GENES; HEALTH RISKS; MICROORGANISMS; SALMONELLA; SURFACE WATERS;

ESCHERICHIA COLI;

INTIMIN; VEROTOXIN 1; VEROTOXIN 2;

MICROBIOLOGY; MICROORGANISM; PATHOGEN; POLYMERASE CHAIN REACTION; SURFACE WATER;

ARTICLE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM DETECTION; CLOSTRIDIUM PERFRINGENS; COLIFORM BACTERIUM; COLONY FORMING UNIT; CONTROLLED STUDY; CULTURE MEDIUM; ENTEROCOCCUS; ENTEROHEMORRHAGIC ESCHERICHIA COLI; ESCHERICHIA COLI O157; MICROBIOLOGICAL EXAMINATION; NONHUMAN; POLYMERASE CHAIN REACTION; SALMONELLA; SALMONELLA TYPHIMURIUM; SHIGELLA; TURBIDITY; WATER ANALYSIS; WATER CONTAMINATION; WATER QUALITY; WATER SAMPLING;

BACTERIAL TYPING TECHNIQUES; ENVIRONMENTAL MONITORING; ESCHERICHIA COLI O157; FRESH WATER; POLYMERASE CHAIN REACTION; SALMONELLA; WATER MICROBIOLOGY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; SALMONELLA; SHIGELLA;

EID: 79959771115     PISSN: 01676369     EISSN: 15732959     Source Type: Journal    
DOI: 10.1007/s10661-010-1650-x     Document Type: Article

References (28)

1. Ahmed, W., Sawant, S., Huygens, F., Goonetilleke, A., & Gardner, T. (2009). Prevalence and occurrence of zoonotic bacterial pathogens in surface waters determined by quantitative PCR. Water Research, 43, 4918-4928.
2. Avery, L. M., Williams, A. P., Killham, K., & Jones, D. L. (2008). Survival of Escherichia coli O157:H7 in waters from lakes, rivers, puddles and animal-drinking troughs. Science of the Total Environment, 389, 378-385. (Pubitemid 350101747)
3. Bertrand, R., & Roig, B. (2007). Evaluation of enrichment-free PCR-based detection on the rfbE gene of Escherichia coli O157 - Application to municipal wastewater. Water Research, 41, 1280-1286.
4. Collazo, C. M., & Galan, J. E. (1997). The invasion-associated type-III protein secretion system in Salmonella - a review. Gene, 192, 51-59. (Pubitemid 27267479)
5. Dharmasiri, U., Witek, M. A., Adams, A. A., Osiri, J. K., Hupert, M. L., Bianchi, T. S., et al. (2010). Enrichment and detection of Escherichia coli O157:H7 from water samples using an antibody modified microfluidic chip. Analytical Chemistry, 82, 2844-2849.
6. Dorner, S. M., Anderson, W. B., Gaulin, T., Candon, H. L., Slawson, R. M., Payment, P., et al. (2007). Pathogen and indicator variability in a heavily impacted watershed. Journal of Water and Health, 05.2, 241-257. (Pubitemid 46929258)
7. Duris, J. W., Haack, S. K., & Fogarty, L. R. (2009). Gene and antigen markers of Shiga-toxin producing E.coli from Michigan and Indiana river water: occurrence and relation to recreation al water quality criteria. Journal of Environmental Quality, 38, 1878-1886.
8. EFSA (2010). The community summary report on trends and sources of zoonoses and zoonotic agents in the European Union in 2008. The EFSA Journal, 1496.
9. Feder, I., Nietfeld, J. C., Galland, J., Yeary, T., Sargent, J. M., Oberst, R., et al. (2001). Comparison of cultivation and PCR-hybridization for detection of Salmonella in porcine fecal and water samples. Journal of Clinical Microbiology, 39, 2477-2484. (Pubitemid 32606222)
10. Hrudey, S. E., & Hrudey, E. J. (2004). Safe drinking water: Lessons from recent outbreaks in af f luent nations. London: IWA.
11. Hrudey, S. E., Payment, P., Huck, P. M., Gillham, R. W., & Hrudey, E. J. (2003). A fatal waterborne disease epidemic in Walkerton, Ontario: Comparison with other waterborne outbreak in the developed world. Water Science and Technology, 47, 7-14.
12. Hu, Y., Zhang, Q., & Meitzer, J. C. (1999). Rapid and sensitive detection of Escherichia coli O157:H7 in bovine faeces by a multiplex PCR. Journal of Applied Microbiology, 87, 867-876. (Pubitemid 30055700)
13. ISS (2007a). Metodi Analitici di riferimento per le acque destinate al consumo umano ai sensi del DL.vo 31/2001. Metodi Microbiologici. Edited by: L. Bonadonna, M. Ottaviani. Rapporti ISTISAN, 07/5, Istituto Superiore di Sanità.
14. ISS (2007b). Metodi Analitici di riferimento per le acque destinate al consumo umano ai sensi del DL.vo 31/2001. Metodi Chimici. Edited by: L. Bonadonna, M. Ottaviani. Rapporti ISTISAN, 07/31, Istituto Superiore di Sanità.
15. James, C. E., Stanley, K. N., Allison, H. E., Flint, H. J., Stewart, C. S., Sharp, R. J., et al. (2001). Lytic and lysogenic infection of diverse Escherichia coli and Shigella strains with a verocytotoxigenic bacteriophage. Applied and Environmental Microbiology, 67, 4335-4337. (Pubitemid 33641734)
16. Kuhnert, P., Boerlin, P., & Frey, J. (2000). Target genes for virulence assessment of E. coli isolates from water, food and the environment. FEMS Microbiology Reviews, 24, 107-117. (Pubitemid 30047117)
17. Lemarchand, K., & Lebaron, P. (2003). Occurrence of Salmonella spp. and Cryptosporidium spp. in French coastal watershed: relationship with fecal indicators. FEMS Microbiology Letters, 218, 203-209. (Pubitemid 36173549)
18. Liu, Y., Wang, C., Tyrrell, G., & Li, X. F. (2010). Production of Shiga-like toxins in viable but non culturable Escherichia coli O157:H7. Water Research, 44, 711-718.
19. Malorny, B., Hoofar, J., Bunge, C., & Helmuth, R. (2003). Multicenter validation of the analytical accuracy of Salmonella PCR: towards and international standard. Applied and Environmental Microbiology, 69, 290-296. (Pubitemid 36077487)
20. Marucci, P. L., Olivera, N. L., Brugnoni, L. I., Sica, M. G., & Cubitto, M. A. (2010). The occurrence of Shiga toxin-producing Escherichia coli in bathing water of the Sierra de la Ventana region, Buenos Aires Province, Argentina. Environmental Monitoring and Assessment. doi:10.1007/s10661-010-1488- 2.
21. Moganedi, K. L. M., Goyvaerts, E. M. A., Venter, S. N., & Sibara, M. M. (2007). Optimization of the PCR-invA primers for the detection of Salmonella in drinking and surface waters following a pre-cultivation step. Water SA, 33, 195-202. (Pubitemid 46683235)
22. Mull, B., & Hill, V. R. (2009). Recovery and detection of Escherichia coli O157:H7 in surface water, using ultrafiltration and Real-Time PCR. Applied and Environmental Microbiology, 75, 3593-3597.
23. Nwachuku, N., & Gerba, C. P. (2008). Occurence and persistence of Escherichia coli O157:H7 in water. Reviews in Environmental Science and Biotechnology, 7, 267-273.
24. Shelton, D. R., Karns, J. S., Higgins, J. A., Van Kessel, J. A., Perdue, M. L., Belt, K. T., et al. (2006). Inpact of microbial diversity on rapid detection of enterohemorragic Escherichia coli in surface waters. FEMS Microbiology Letters, 261, 95-101. (Pubitemid 43999707)
25. Strauch, E., Hammerl, J. A., Konietzny, A., Schneiker-Bekel, S., Arnold, W., Goesmann, A., et al. (2008). Bacteriophage 2851 is a prototype phage for dissemination of the Shiga toxin variant gene 2c in Escherichia coli O157:H7. Infection and Immunity, 76, 5466-5477.
26. Sugumar, G., Chrisolite, B., Velayutham, P., Selvan, A., & Ramesh, U. (2008). Occurence and season variation of bacterial indicators of faecal pollution along Thoothukudi Coast, Tamil Nadu. Journal of Environmental Biology, 29, 387-391. (Pubitemid 351829950)
27. Thompson, D. E., Rajal, V. B., De Batz, S., & Wuertz, S. (2006). Detection of Salmonella spp. in water using magnetic capture hybridization combined with PCR or Real-Time PCR. Journal of Water and Health, 04.1, 67-75.
28. Touron, A., Berthe, T., Pawlak, B., & Petit, F. (2005). Detection of Salmonella in environmental water and sediment by a nested-multiplex polymerase chain reaction assay. Research in Microbiology, 156, 541-553. (Pubitemid 40615422)