Combined molecular genetic and cytogenetic analysis from single cells after isothermal whole-genome amplification

Clinical Chemistry

Volumn 57, Issue 7, 2011, Pages 1032-1041

  Kroneis, Thomas ^a   Geigl, Jochen B ^a   El Heliebi, Amin ^a   Auer, Martina ^a   Ulz, Peter ^a   Schwarzbraun, Thomas ^a   Dohr, Gottfried ^a   Sedlmayr, Peter ^a  

^a MEDICAL UNIVERSITY OF GRAZ   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOKERATIN; DNA; GENOMIC DNA;

ARTICLE; CELL LOSS; CHROMOSOME ANALYSIS; COLON ADENOCARCINOMA; COMPARATIVE GENOMIC HYBRIDIZATION; CONTROLLED STUDY; DNA FINGERPRINTING; GENE AMPLIFICATION; GENE LOCUS; GENE SEQUENCE; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; ISOTHERMAL TITRATION CALORIMETRY; ISOTHERMAL WHOLE GENOME AMPLIFICATION; LASER MICRODISSECTION; MALE; METAPHASE; MICROCHIMERISM; MOLECULAR GENETICS; NORMAL HUMAN; PERIPHERAL BLOOD MONONUCLEAR CELL; SINGLE CELL ANALYSIS; SINGLE NUCLEOTIDE POLYMORPHISM;

CHIMERISM; CHROMOSOME ABERRATIONS; COMPARATIVE GENOMIC HYBRIDIZATION; CYTOGENETIC ANALYSIS; DNA FINGERPRINTING; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE; GENOME, HUMAN; HT29 CELLS; HUMANS; KERATINS; LEUKOCYTES, MONONUCLEAR; METAPHASE; NEOPLASTIC CELLS, CIRCULATING; NUCLEIC ACID AMPLIFICATION TECHNIQUES; PREGNANCY; PRENATAL DIAGNOSIS; SEQUENCE ANALYSIS, DNA; SINGLE-CELL ANALYSIS;

EID: 79959758185     PISSN: 00099147     EISSN: 15308561     Source Type: Journal    
DOI: 10.1373/clinchem.2011.162131     Document Type: Article

References (16)

1. Nelson JL. Your cells are my cells. Sci Am 2008;298:64-71.
2. Christensen B, Philip J, Kolvraa S, Lykke-Hansen L, Hromadnikova I, Gohel D, et al. Fetal cells in maternal blood: a comparison of methods for cell isolation and identification. Fetal Diagn Ther 2005;20:106-12. (Pubitemid 40278653)
3. Guetta E, Gutstein-Abo L, Barkai G. Trophoblasts isolated from the maternal circulation: in vitro expansion and potential application in noninvasive prenatal diagnosis. J Histochem Cytochem 2005;53:337-9. (Pubitemid 40344069)
4. Hennerbichler S, Kroisel PM, Zierler H, Pertl B, Wintersteiger R, Dohr G, Sedlmayr P. Fetal nucleated red blood cells in peripheral blood of preg- nant women: detection and determination of location on a slide using laser-scanning cytometry. Prenat Diagn 2003;23:710-5. (Pubitemid 37158460)
5. Huang R, Barber TA, Schmidt MA, Tompkins RG, Toner M, Bianchi DW, et al. A microfluidics approach for the isolation of nucleated red blood cells (NRBCs) from the peripheral blood of pregnant women. Prenat Diagn 2008;28:892-9.
6. Mavrou A, Kolialexi A, Antsaklis A, Korantzis A, Metaxotou C. Identification of fetal nucleated red blood cells in the maternal circulation during pregnancy using anti-hemoglobin-epsilon antibody. Fetal Diagn Ther 2003;18:309-13. (Pubitemid 36993936)
7. Vona G, Beroud C, Benachi A, Quenette A, Bonnefont JP, Romana S, et al. Enrichment, immunomorphological, and genetic characterization of fetal cells circulating in maternal blood. Am J Pathol 2002;160:51-8. (Pubitemid 34052265)
8. Weichel W, Irlenbusch S, Kato K, Radbruch A. Sorting of rare cells. In: Radbruch A, ed. Flow cytometry and cell sorting. Berlin: Springer- Verlag; 1992. p 159-67.
9. Ponnusamy S, Mohammed N, Ho SS, Zhang HM, Chan YH, Ng YW, et al. In vivo model to determine fetal-cell enrichment efficiency of novel noninvasive prenatal diagnosis methods. Prenat Diagn 2008;28:494-502.
10. Kroneis T, Gutstein-Abo L, Kofler K, Hartmann M, Hartmann P, Alunni-Fabbroni M, et al. Automatic retrieval of single microchimeric cells and verification of identity by on-chip multiplex PCR. J Cell Mol Med 2010;14:954-69.
11. Gangnus R, Langer S, Breit E, Pantel K, Speicher MR. Genomic profiling of viable and proliferative micrometastatic cells from early-stage breast cancer patients. Clin Cancer Res 2004;10:3457-64. (Pubitemid 38685453)
12. James LA. Comparative genomic hybridization as a tool in tumour cytogenetics. J Pathol 1999;187:385-95. (Pubitemid 29118268)
13. Rohlin A, Wernersson J, Engwall Y, Wiklund L, Bjork J, Nordling M. Parallel sequencing used in detection of mosaic mutations: comparison with four diagnostic DNA screening techniques. Hum Mutat 2009;30:1012-20.
14. Knutsen T, Padilla-Nash HM, Wangsa D, Barenboim-Stapleton L, Camps J, McNeil N, et al. Definitive molecular cytogenetic characterization of 15 colorectal cancer cell lines. Genes Chromosomes Cancer 2010;49:204-23.
15. Ghadimi BM, Sackett DL, Difilippantonio MJ, Schrock E, Neumann T, Jauho A, et al. Centrosome amplification and instability occurs exclusively in aneuploid, but not in diploid colorectal cancer cell lines, and correlates with numerical chromosomal aberrations. Genes Chromosomes Cancer 2000;27:183-90. (Pubitemid 30030317)
16. Kawai K, Viars C, Arden K, Tarin D, Urquidi V, Goodison S. Comprehensive karyotyping of the HT-29 colon adenocarcinoma cell line. Genes Chromosomes Cancer 2002;34:1-8. (Pubitemid 34260590)