PP1 forms an active complex with TLRR (lrrc67), a putative PP1 regulatory subunit, during the early stages of spermiogenesis in mice

PLoS ONE

Volumn 6, Issue 6, 2011, Pages

  Wang, Rong ^a   Sperry, Ann O ^a  

^a EAST CAROLINA UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATASE; BINDING PROTEIN; KINESIN 1B; PHOSPHOPROTEIN PHOSPHATASE 1; TESTIS LEUCINE RICH REPEAT PROTEIN; TUBULIN; UNCLASSIFIED DRUG; SIGNAL PEPTIDE; TLRR PROTEIN, MOUSE;

ANIMAL TISSUE; ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; CYTOSKELETON; DEVELOPMENTAL STAGE; ENZYME ACTIVITY; MALE; MOUSE; NONHUMAN; PACHYTENE; PROTEIN PHOSPHORYLATION; SEMINIFEROUS TUBULE EPITHELIUM; SPERMATID; SPERMATOCYTE; SPERMATOGENESIS; TESTIS DEVELOPMENT; ANIMAL; GENETICS; IMMUNOPRECIPITATION; METABOLISM; PHYSIOLOGY; PROTEIN BINDING; TESTIS; WESTERN BLOTTING;

MAMMALIA; MURINAE; MUS;

ANIMALS; BLOTTING, WESTERN; IMMUNOPRECIPITATION; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; MALE; MICE; PROTEIN BINDING; PROTEIN PHOSPHATASE 1; SPERMATOGENESIS; TESTIS;

EID: 79959732712     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0021767     Document Type: Article

References (26)

1. Wolosewick JJ, Bryan JHD, (1977) Ultrastructural characterization of the manchette microtubules in the seminiferous epithelium of the mouse. Am J Anat 150: 301-331.
2. Cole A, Meistrich ML, Cherry LM, Trostle-Weige PK, (1988) Nuclear and manchette development in spermatids of normal and azh/azh mutant mice. Biol Reprod 38: 385-401.
3. Meistrich ML, Trostle-Weige PK, Russell LD, (1990) Abnormal manchette development in spermatids of azh/azh mutant mice. Am J Anat 188: 74-86.
4. Kierszenbaum AL, (2002) Intramanchette transport (IMT): managing the making of the spermatid head, centrosome, and tail. Mol Reprod Dev 63: 1-4.
5. Wang R, Sperry AO, (2008) Identification of a novel leucine-rich repeat protein and candidate PP1 regulatory subunit expressed in developing spermatids. BMC Cell Biol 9: 9.
6. Wang R, Kaul A, Sperry AO, (2010) TLRR (lrrc67) interacts with PP1 and is associated with a cytoskeletal complex in the testis. Biol Cell 102: 173-189.
7. Oppedisano-Wells L, Varmuza S, (2003) Protein phosphatase 1cγ is required in germ cells in murine testis. Mol Reprod Dev 65: 157-166.
8. Chakrabarti R, Kline D, Lu J, Orth J, Pilder S, et al. (2007) Analysis of Ppp1cc-null mice suggests a role for PP1gamma2 in sperm morphogenesis. Biol Reprod 76: 992-1001.
9. Cheng L, Pilder S, Nairn AC, Ramdas S, Vijayaraghavan S, (2009) PP1γ2 and PPP1R11 are parts of a multimeric complex in developing testicular germ cells in which their steady state levels are reciprocally related. PLoS ONE 4: e4861.
10. Hrabchak C, Varmuza S, (2004) Identification of the spermatogenic zip protein Spz1 as a putative protein phosphatase-1 (PP1) regulatory protein that specifically binds the PP1cgamma2 splice variant in mouse testis. J Biol Chem 279: 37079-37086.
11. Chakrabarti R, Cheng L, Puri P, Soler D, Vijayaraghavan S, (2007) Protein phosphatase PP1γ2 in sperm morphogenesis and epididymal initiation of sperm motility. Asian J Androl 9: 445-452.
12. Mishra S, Somanath PR, Huang Z, Vijayaraghavan S, (2003) Binding and inactivation of the germ cell-specific protein phosphatase PP1γ2 by sds22 during epididymal sperm maturation. Biol Reprod 69: 1572-1579.
13. Huang Z, Vijayaraghavan S, (2004) Increased phosphorylation of a distinct subcellular pool of protein phosphatase, PP1γ2, during epididymal sperm maturation. Biol Reprod 70: 439-447.
14. Cherry LM, Hsu TC, (1984) Antitubulin immunofluorescence studies of spermatogenesis in the mouse. Chromosoma 90: 265-274.
15. Moreno RD, Schatten G, (2000) Microtubule configurations and post-translational alpha-tubulin modifications during mammalian spermatogenesis. Cell Motil Cytoskeleton 46: 235-246.
16. Bellve AR, Millette CF, Bhatnagar YM, O'Brien DA, (1977) Dissociation of the mouse testis and characterization of isolated spermatogenic cells. J Histochem Cytochem 25: 480-494.
17. Varmuza S, Jurisicova A, Okano K, Hudson J, Boekelheide K, et al. (1999) Spermiogenesis is impaired in mice bearing a targeted mutation in the protein phosphatase 1cγ gene. Dev Biol 205: 98-110.
18. Shima H, Haneji T, Hatano Y, Kasugai I, Sugimura T, et al. (1993) Protein phosphatase 1γ2 is associated with nuclei of meiotic cells in rat testis. Biochem Biophys Res Commun 194: 930-937.
19. Henderson H, MacLeod G, Hrabchak C, Varmuza S, (2010) New candidate targets of protein phosphatase-1c-gamma-2 in mouse testis revealed by a differential phosphoproteome analysis. Int J Androl pp. 1-13.
20. Morfini GA, You YM, Pollema SL, Kaminska A, Liu K, et al. (2009) Pathogenic huntingtin inhibits fast axonal transport by activating JNK3 and phosphorylating kinesin. Nat Neurosci 12: 864-871.
21. McClintock TS, Glasser CE, Bose SC, Bergman DA, (2008) Tissue expression patterns identify mouse cilia genes. Physiol Genomics 32: 198-206.
22. Li JB, Gerdes JM, Haycraft CJ, Fan Y, Teslovich TM, et al. (2004) Comparative genomics identifies a flagellar and basal body proteome that includes the BBS5 human disease gene. Cell 117: 541.
23. Su AI, Cooke MP, Ching KA, Hakak Y, Walker JR, et al. (2002) Large-scale analysis of the human and mouse transcriptomes. Proc Natl Acad Sci USA 99: 4465-4470.
24. Wang W, Brautigan DL, (2008) Phosphatase inhibitor 2 promotes acetylation of tubulin in the primary cilium of human retinal epithelial cells. BMC Cell Biol 9: 62.
25. Vijayaraghavan S, Stephens DT, Trautman K, Smith GD, Khatra B, et al. (1996) Sperm motility development in the epididymis is associated with decreased glycogen synthase kinase-3 and protein phosphatase 1 activity. Biol Reprod 54: 709-718.
26. DeBoer SR, You Y, Szodorai A, Kaminska A, Pigino G, et al. (2008) Conventional kinesin holoenzymes are composed of heavy and light chain homodimers. Biochemistry 47: 4535-4543.