Cytometry of Intracellular Signaling: From Laboratory Bench to Clinical Application

Methods in Cell Biology

Volumn 103, Issue , 2011, Pages 203-220

  Hedley, David W ^a   Chow, Sue ^a   Shankey, T Vincent ^b  

^a PRINCESS MARGARET HOSPITAL   (Canada)

^b BECKMAN COULTER INC   (United States)

Author keywords

Activator; Antibody; Inhibitor; Leukemia; Lysis; Signaling pathway

Indexed keywords

FIXATIVE; FLUORESCENT DYE; LIPOPOLYSACCHARIDE; METHANOL; PHORBOL 13 ACETATE 12 MYRISTATE; PHOSPHOPROTEIN; PHOSPHOSPECIFIC ANTIBODY; PROTEIN KINASE INHIBITOR; SIGNAL PEPTIDE;

ACUTE GRANULOCYTIC LEUKEMIA; CHEMISTRY; DRUG ANTAGONISM; DRUG POTENTIATION; FLOW CYTOMETRY; HUMAN; IMAGE CYTOMETRY; METABOLISM; METHODOLOGY; PATHOLOGY; PHOSPHORYLATION; REVIEW; SIGNAL TRANSDUCTION; STAINING; TISSUE FIXATION;

ANTIBODIES, PHOSPHO-SPECIFIC; FIXATIVES; FLOW CYTOMETRY; FLUORESCENT DYES; HUMANS; IMAGE CYTOMETRY; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; LEUKEMIA, MYELOID, ACUTE; LIPOPOLYSACCHARIDES; METABOLIC NETWORKS AND PATHWAYS; METHANOL; PHOSPHOPROTEINS; PHOSPHORYLATION; PROTEIN KINASE INHIBITORS; SIGNAL TRANSDUCTION; STAINING AND LABELING; TETRADECANOYLPHORBOL ACETATE; TISSUE FIXATION;

EID: 79959719797     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/B978-0-12-385493-3.00009-7     Document Type: Book

References (16)

1. Chow S., Hedley D., Grom P., Magari R., Jacobberger J.W., Shankey T.V. Whole blood fixation and permeabilization protocol with red blood cell lysis for flow cytometry of intracellular phosphorylated epitopes in leukocyte subpopulations. Cytometry A 2005, 67(1):4-17.
2. Chow, S., Hedley, D., and Shankey, T. V. (2008). Whole blood processing for measurement of signaling proteins by flow cytometry. Curr. Prot. Cytom. Chapter 9, Unit 9.27.
3. Chow S., Minden M.D., Hedley D.W. Constitutive phosphorylation of the S6 ribosomal protein via mTOR and ERK signaling in the peripheral blasts of acute leukemia patients. Exp. Hematol. 2006, 34(9):1183-1191.
4. Chow S., Patel H., Hedley D.W. Measurement of MAP kinase activation by flow cytometry using phospho-specific antibodies to MEK and ERK: potential for pharmacodynamic monitoring of signal transduction inhibitors. Cytometry 2001, 46(2):72-78.
5. Hedley D.W., Chow S., Goolsby C., Shankey T.V. Pharmacodynamic monitoring of molecular-targeted agents in the peripheral blood of leukemia patients using flow cytometry. Toxicol. Pathol. 2008, 36(1):133-139.
6. Irish J.M., Czerwinski D.K., Nolan G.P., Levy R. Kinetics of B cell receptor signaling in human B cell subsets mapped by phosphospecific flow cytometry. J. Immunol. 2006, 177(3):1581-1589.
7. Irish J.M., Hovland R., Krutzik P.O., Perez O.D., Bruserud O., Gjertsen B.T., et al. Single cell profiling of potentiated phospho-protein networks in cancer cells. Cell 2004, 118(2):217-228.
8. Jacobberger J.W., Sramkoski R.M., Frisa P.S., Ye P.P., Gottlieb M.A., Hedley D.W., et al. Immunoreactivity of Stat5 phosphorylated on tyrosine as a cell-based measure of Bcr/Abl kinase activity. Cytometry 2003, 54A(2):75-88.
9. Kotecha N., Flores N.J., Irish J.M., Simonds E.F., Sakai D.S., Archambeault S., et al. Single-cell profiling identifies aberrant STAT5 activation in myeloid malignancies with specific clinical and biologic correlates. Cancer Cell 2008, 14(4):335-343.
10. Krutzik P.O., Nolan G.P. Intracellular phospho-protein staining techniques for flow cytometry: monitoring single cell signaling events. Cytometry A 2003, 55(2):61-70.
11. Lu Y.C., Yeh W.C., Ohashi P.S. LPS/TLR4 signal transduction pathway. Cytokine 2008, 42(2):145-151.
12. Perez O.D., Nolan G.P. Simultaneous measurement of multiple active kinase states using polychromatic flow cytometry. Nat. Biotechnol. 2002, 20(2):155-162.
13. Raynaud F.I., Eccles S.A., Patel S., Alix S., Box G., Chuckowree I., et al. Biological properties of potent inhibitors of class I phosphatidylinositide 3-kinases: from PI-103 through PI-540, PI-620 to the oral agent GDC-0941. Mol. Cancer Ther. 2009, 8(7):1725-1738.
14. Schwock J., Ho J.C., Luther E., Hedley D.W., Geddie W.R. Measurement of signaling pathway activities in solid tumor fine-needle biopsies by slide-based cytometry. Diagn. Mol. Pathol. 2007, 16(3):130-140.
15. Shankey T.V., Forman M., Scibelli P., Cobb J., Smith C.M., Mills R., et al. An optimized whole blood method for flow cytometric measurement of ZAP-70 protein expression in chronic lymphocytic leukemia. Cytom. B Clin. Cytom. 2006, 70(4):259-269.
16. Tong F.K., Chow S., Hedley D. Pharmacodynamic monitoring of BAY 43-9006 (Sorafenib) in phase I clinical trials involving solid tumor and AML/MDS patients, using flow cytometry to monitor activation of the ERK pathway in peripheral blood cells. Cytom. B Clin. Cytom. 2006, 70(3):107-114.