Quantification of ceramide levels in mammalian cells by high performance liquid chromatography coupled to tandem mass spectrometry with multiple-reaction-monitoring mode (HPLC-MS/MS-MRM)

Analytical Methods

Volumn 3, Issue 5, 2011, Pages 1193-1197

  Zhang, Kangling ^a   Haynes, Teka Ann S ^a   Filippova, Maria ^a   Filippov, Valery ^a   Duerksen Hughes, Penelope J ^a  

^a LOMA LINDA UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AGILENT; BIOLOGICAL STUDIES; CELL DIFFERENTIATION; CELLULAR FUNCTION; CERAMIDES; DNA DAMAGES; HPLC-MS; LIQUID CHROMATOGRAPHY COUPLED TO TANDEM MASS SPECTROMETRY; MAMMALIAN CELLS; REPRODUCIBILITIES; TRIPLE-QUADRUPOLE MASS SPECTROMETER; VALIDATED METHODS;

AMIDES; CELL DEATH; MAMMALS; MASS SPECTROMETRY;

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

EID: 79959716331     PISSN: 17599660     EISSN: 17599679     Source Type: Journal    
DOI: 10.1039/c1ay05022b     Document Type: Article

References (24)

1. R. N. Kolesnick F. M. Goni A. Alonso Compartmentalization of ceramide signaling: physical foundations and biological effects J. Cell. Physiol. 2000 184 285 300 (Pubitemid 30625491)
2. R. Bose M. Verheij et al. Ceramide synthase mediates daunorubicin-induced apoptosis: an alternative mechanism for generating death signals Cell 1995 82 405 414
3. T. Yamashita R. Wada et al. A vital role for glycosphingolipid synthesis during development and differentiation Proc. Natl. Acad. Sci. U. S. A. 1999 96 9142 9147 (Pubitemid 29374721)
4. L. M. Obeid C. M. Linardic et al. Programmed cell death induced by ceramide Science 1993 259 1769 1771 (Pubitemid 23114667)
5. M. Moulin S. Carpentier et al. Potential roles of membrane fluidity and ceramide in hyperthermia and alcohol stimulation of TRAIL apoptosis Apoptosis 2007 12 1703 1720 (Pubitemid 47106895)
6. M. E. Venable J. Y. Lee et al. Role of ceramide in cellular senescence J. Biol. Chem. 1995 270 30701 30708
7. Y. Lee J. Lee J. Cho Cell-permeable ceramides act as novel regulators of U937 cell-cell adhesion mediated by CD29, CD98, and CD147 Immunobiol. 2010 215 294 303
8. S. Bourteele A. Hausser et al. Tumor necrosis factor induces ceramide oscillations and negatively controls sphingolipid synthases by caspases in apoptotic Kym-1 cells J. Biol. Chem. 1998 273 31245 31251 (Pubitemid 28533140)
9. V. Filippov M. Filippova et al. The early response to DNA damage can lead to activation of alternative splicing activity resulting in CD44 splice pattern changes Cancer Res. 2007 67 7621 7630 (Pubitemid 47281354)
10. J. Xu C. H. Yeh et al. Involvement of de novo ceramide biosynthesis in tumor necrosis factor-alpha/cycloheximide-induced cerebral endothelial cell death J. Biol. Chem. 1998 273 16521 16526 (Pubitemid 28311437)
11. Y. A. Hannun Functions of ceramide in coordinating cellular responses to stress Science 1996 274 1855 1859 (Pubitemid 26424756)
12. V. Mansat G. Laurent et al. The protein kinase C activators phorbol esters and phosphatidylserine inhibit neutral sphingomyelinase activation, ceramide generation, and apoptosis triggered by daunorubicin Cancer Res 1997 57 5300 5304 (Pubitemid 27516367)
13. S. Schutze K. Potthoff et al. TNF activates NF-kappa B by phosphatidylcholine-specific phospholipase C-induced "acidic" sphingomyelin breakdown Cell 1992 71 765 776
14. Y. Hsu K. Chi et al. Ceramide inhibits lipopolysaccharide-mediated nitric oxide synthase and cyclooxygenase-2 induction in macrophages: effects on protein kinases and transcription factors J. Immunol. 2001 166 5388 5397 (Pubitemid 32374158)
15. W. Berghe K. De Bosscher et al. The nuclear Factor-κB engages CBP/p300 and histone acetyltransferase activity for transcriptional activation of the inerleukin-6 gene promoter J. Biol. Chem. 1999 274 32091 32098
16. C. C. Geilen C. E. Wieder et al. Regulatory role in cell proliferation, differentiation, and apoptosis in human epidermis Arch. Dermatol. Res. 1997 289 559 566 (Pubitemid 27477647)
17. B. N. Ames D. T. Dubin The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid J. Biol. Chem. 1960 235 769 775
18. D. A. Kennerly C. W. Parker T. J. Sullivan Use of diacylglycerol kinase to quantitate picomole levels of 1, 2-diacylglycerol Anal. Biochem. 1979 98 123 131 (Pubitemid 10235485)
19. T. A. Haynes P. J. Duerksen-Hughes et al. C18 ceramide analysis in mammalian cells employing reversed-phase high-performance liquid chromatography tandem mass spectrometry Anal. Biochem. 2008 378 80 86
20. M. C. Sullards Analysis of sphingomyelin, glucosylceramide, ceramide, sphingosine, and sphingosine 1-phosphate by tendem mass spectrometry Methods Enzymol. 2000 312 32 45
21. M. C. Sullards A. H. Merrill, Jr. Analysis of sphingosine 1-phosphate, ceramides, and other bioactive sphingolipids by high-performance liquid chromatography-tandem mass spectrometry Sci. STKE 2001 p. 11
22. J. Bielawski J. S. Pierce J. Snider B. Rembiesa Z. M. Szulc A. Bielawska Comprehensive quantitative analysis of bioactive sphingolipids by high-performance liquid chromatography-tandem mass spectrometry Methods Mol. Biol. 2009 579 443 467
23. R. L. Shaner J. C. Allegood H. Park E. Wang S. Kelly C. A. Haynes M. C. Sullards A. H. Merrill, Jr. Quantitative analysis of sphingolipids for lipidomics using triple quadruple and quadruple linear ion trap mass spectrometers J. Lipid Res. 2009 50 1692 1707
24. www.lipidmaps.org