Directional, seamless, and restriction enzyme-free construction of random-primed complementary DNA libraries using phosphorothioate-modified primers

Analytical Biochemistry

Volumn 416, Issue 1, 2011, Pages 141-143

  Howland, Shanshan W ^a   Poh, Chek Meng ^a   Rénia, Laurent ^a  

^a AGENCY FOR SCIENCE   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

CLONE CELLS; CLONING; ENZYMES; ESCHERICHIA COLI;

COMBINED ACTIONS; COMPLETE COVERAGES; DIRECTIONAL CLONING; EXONUCLEASE ACTIVITY; FREE CONSTRUCTION; PHOSPHOROTHIOATES; RESTRICTION ENZYMES; SIZE INDEPENDENTS;

DNA;

COMPLEMENTARY DNA; DNA DIRECTED DNA POLYMERASE ALPHA; EXODEOXYRIBONUCLEASE III; EXODEOXYRIBONUCLEASE V; PHOSPHOROTHIOIC ACID; PRIMER DNA; RESTRICTION ENDONUCLEASE;

ARTICLE; DNA LIBRARY; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION;

ESCHERICHIA COLI;

EID: 79959693832     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.04.006     Document Type: Article

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