A novel approach to dual excitation ratiometric optical mapping of cardiac action potentials with Di-4-ANEPPS using pulsed led excitation

IEEE Transactions on Biomedical Engineering

Volumn 58, Issue 7, 2011, Pages 2120-2126

  Bachtel, Andrew D ^a   Gray, Richard A ^d   Stohlman, Jayna M ^d   Bourgeois, Elliot B ^b   Pollard, Andrew E ^c   Rogers, Jack M ^c  

^a Respiratory and Monitoring Solutions   (United States)

^b CHILDREN S HOSPITAL OF PHILADELPHIA   (United States)

^c UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

^d CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

Author keywords

Light emitting diode; membrane potential; motion artifact; ratiometry

Indexed keywords

ACTION POTENTIALS; ALTERNATING PHASE; BAND PASS; CARDIAC ACTION POTENTIAL; CROSS-CORRELATION COEFFICIENT; DI-4-ANEPPS; EMISSION BANDS; EXCITATION AND EMISSION SPECTRA; EXCITATION BANDS; FLUORESCENCE SIGNALS; LED EXCITATION; MEMBRANE POTENTIAL; MOTION ARTIFACT; OPTICAL MAPPING; RATIOMETRIC; RATIOMETRY; SIGNAL TO NOISE; TRANSMEMBRANE POTENTIALS; WASHING-OUT;

BIOELECTRIC POTENTIALS; ELECTROPHYSIOLOGY; EMISSION SPECTROSCOPY; FLUORESCENCE; LIGHT EMISSION; SIGNAL RECONSTRUCTION; SIGNAL TO NOISE RATIO;

LIGHT EMITTING DIODES;

ANIMAL TISSUE; ARTICLE; ARTIFACT REDUCTION; CAMERA; CONTROLLED STUDY; CORRELATION COEFFICIENT; ELECTROCARDIOGRAPHY; EYE TRACKING; FEASIBILITY STUDY; FLUORESCENCE; HEART EXCITATION; HEART MUSCLE FIBER MEMBRANE POTENTIAL; HEART MUSCLE POTENTIAL; ISOLATED HEART; LIGHT EMITTING DIODE; NONHUMAN; SIGNAL NOISE RATIO; SIGNAL PROCESSING; SWINE;

ACTION POTENTIALS; ANIMALS; ARTIFACTS; FEMALE; HEART; MALE; PYRIDINIUM COMPOUNDS; SIGNAL PROCESSING, COMPUTER-ASSISTED; SUBTRACTION TECHNIQUE; SWINE; VOLTAGE-SENSITIVE DYE IMAGING;

EID: 79959571797     PISSN: 00189294     EISSN: 15582531     Source Type: Journal    
DOI: 10.1109/TBME.2011.2148719     Document Type: Article

References (26)

1. I.R.Efimov,D.T.Huang, J. M. Rendt, and G. Salama, "Optical mapping of repolarization and refractoriness from intact hearts," Circulation, vol. 90, pp. 1469-1480, 1994. (Pubitemid 24284468)
2. R. Brandes, V. M. Figueredo, S. A. Camacho, B. M. Massie, and M. W.Weiner, "Suppression of motion artifacts in fluorescence spectroscopy of perfused hearts," Am. J. Phys. Heart Circ. Physiol., vol. 263, pp. H972-980, 1992.
3. D. C.-S. Tai, B. J. Caldwell, I. J. LeGrice, D. A. Hooks, A. J. Pullan, and B. H. Smaill, "Correction of motion artifact in transmembrane voltagesensitive fluorescent dye emission in hearts," Am. J. Physiol. Heart Circ. Physiol., vol. 287, pp. H985-H993, 2004. (Pubitemid 39096642)
4. T. Fukano, S. Shimozono, and A.Miyawaki, "Fast dual-excitation ratiometry with light-emitting diodes and high-speed liquid crystal shutters," Biochem. Biophys. Res. Commun., vol. 340, pp. 250-255, 2006. (Pubitemid 41827673)
5. U. Hoger, P. H. Torkkeli, and A. S. French, "Ratiometric calcium concentration estimation using LED excitation during mechanotransduction in single sensory neurons," J. Neurosci. Methods, vol. 164, pp. 255-260, 2007. (Pubitemid 47016654)
6. A. Bullen and P. Saggau, "High-speed, random-access fluorescence microscopy: II. Fast quantitative measurements with voltage-sensitive dyes," Biophys. J., vol. 76, pp. 2272-2287, 1999. (Pubitemid 29266381)
7. V. Montana, D. L. Farkas, and L. M. Loew, "Dual-wavelength ratiometric fluorescence measurements of membrane potential," Biochemistry, vol. 28, pp. 4536-4539, 1989. (Pubitemid 19151021)
8. M. Nishimura, H. Shirasawa, and W.-J. Song, "A light-emitting diode light source for imaging of neural activities with voltage-sensitive dyes," Neurosci. Res., vol. 54, pp. 230-234, 2006. (Pubitemid 43276081)
9. M.W. Kay, G. P.Walcott, J. D. Gladden, S. B. Melnick, and J. M. Rogers, "Lifetimes of epicardial rotors in panoramic optical maps of fibrillating swine ventricles," Am. J. Physiol. Heart Circ. Physiol., vol. 291, pp. H1935-H1941, 2006. (Pubitemid 44497702)
10. E. B. Bourgeois, V. G. Fast, R. L. Collins, J. D. Gladden, and J.M. Rogers, "Change in conduction velocity due to fiber curvature in cultured neonatal rat ventricular myocytes," IEEE Trans Biomed. Eng., vol. 56, no. 3, pp. 855-61, Mar. 2009.
11. E. Entcheva, Y. Kostov, E. Tchernev, and L. Tung, "Fluorescence imaging of electrical activity in cardiac cells using an all-solid-state system," IEEE Trans. Biomed. Eng., vol. 51, no. 2, pp. 333-341, Feb. 2004. (Pubitemid 38133613)
12. T. Nishigaki, C. D.Wood, K. Shiba, S. A. Baba, and A. Darszon, "Stroboscopic illumination using light-emitting diodes reduces phototoxicity in fluorescence cell imaging," Biotechniques, vol. 41, pp. 191-197, 2006. (Pubitemid 46542603)
13. T. Fukano, S. Shimozono, and A. Miyawaki, "Development ofmicroscopic systems for high-speed dual-excitation ratiometric Ca2+ imaging," Brain Cell. Biol., vol. 36, pp. 43-52, 2008.
14. H. Qin, M. W. Kay, N. Chattipakorn, D. T. Redden, R. E. Ideker, and J. M. Rogers, "Effects of heart isolation, voltage-sensitive dye, and electromechanical uncoupling agents on ventricular fibrillation," Am. J. Physiol.. Heart Circulatory Physiol., vol. 284, pp. H1818-H1826, 2003. (Pubitemid 36460799)
15. L. M. Loew, "Design and characterization of electrochromic membrane probes," J. Biochem. Biophys. Methods, vol. 6, pp. 243-260, 1982.
16. P. Fromherz and A. Lambacher, "Spectra of voltage-sensitive fluorescence of styryl-dye in neuron membrane," Biochim. Biophys. Acta., pp. 149-156, 1991.
17. P. L. Johnson, W. Smith, T. C. Baynham, and S. B. Knisley, "Errors caused by combination of Di-4 ANEPPS and Fluo3/4 for simultaneous measurements of transmembrane potentials and intracellular calcium," Ann. Biomed. Eng., vol. 27, pp. 563-571, 1999. (Pubitemid 129585010)
18. W. Y. Kao, C. E. Davis, Y. I. Kim, and J. M. Beach, "Fluorescence emission spectral shift measurements of membrane potential in single cells," Biophys. J., vol. 81, pp. 1163-1170, 2001. (Pubitemid 32721484)
19. S. B. Knisley, R. K. Justice, W. Kong, and P. L. Johnson, "Ratiometry of transmembrane voltage-sensitive fluorescent dye emission in hearts," Am. J. Physiol. Heart Circ. Physiol., vol. 279, pp. H1421-H1433, 2000.
20. E. Gross, R. S. Bedlack, Jr., and L. M. Loew, "Dual-wavelength ratiometric fluorescence measurement of the membrane dipole potential," Biophys. J., vol. 67, pp. 208-16, 1994. (Pubitemid 24197886)
21. Y. Hayashi, M. M. Zviman, J. G. Brand, J. H. Teeter, and D. Restrepo, "Measurement of membrane potential and [Ca2+]i in cell ensembles: Application to the study of glutamate taste in mice," Biophys. J., vol. 71, pp. 1057-1070, 1996. (Pubitemid 26289187)
22. K. R. Laurita and A. Singal, "Mapping action potentials and calcium transients simultaneously from the intact heart," Am. J. Physiol. Heart Circ. Physiol., vol. 280, pp. H2053-H2060, 2001.
23. M. F. Vitha and R. J. Clarke, "Comparison of excitation and emission ratiometric fluorescence methods for quantifying the membrane dipole potential," Biochim. Biophys. Acta., vol. 1768, pp. 107-114, 2007. (Pubitemid 44909072)
24. E. Fluhler, V. G. Burnham, and L.M. Loew, "Spectra, membrane binding, and potentiometric responses of new charge shift probes," Biochemistry, vol. 24, pp. 5749-5755, 1985. (Pubitemid 16203012)
25. A. Savitzky and M. J. E. Golay, "Smoothing and differentiation of data by simplified least squares procedures," Anal. Chem., vol. 36, pp. 1627-1639, 1964.
26. N. H. Brown, H. M. Dobrovolny, D. J. Gauthier, and P. D.Wolf, "A fiberbased ratiometric optical cardiac mapping channel using a diffraction grating and split detector," Biophys. J., vol. 93, pp. 254-263, 2007. (Pubitemid 47041670)