Expression, purification, and characterization of protective MPT64 antigen protein and identification of its multimers isolated from nontoxic Mycobacterium tuberculosis H37Ra

Biotechnology and Applied Biochemistry

Volumn 58, Issue 3, 2011, Pages 185-189

  Chu, Teng Ping J ^a   Yuann, Jeu Ming P ^a  

^a MING CHUAN UNIVERSITY   (Taiwan)

Author keywords

circular dichroism; MPT64; Mycobacterium tuberculosis; nontoxic H37Ra; secondary structures

Indexed keywords

ANTIGEN PROTEINS; BIOPHYSICAL STUDIES; DISULFIDE BONDS; GENE CLONES; MATRIX ASSISTED LASER DESORPTION; MPT64; MULTIMERS; MYCOBACTERIUM TUBERCULOSIS; MYCOBACTERIUM TUBERCULOSIS H37RA; NONTOXIC H37RA; PLASMID DNA; PROTECTIVE ANTIGENS; PROTEIN-PROTEIN INTERACTIONS; RANDOM COIL; SECONDARY STRUCTURES; SECRETED PROTEIN; TETRAMERS; WESTERN BLOTS;

ANTIGENS; CLONING; COVALENT BONDS; DESORPTION; DICHROISM; GENES; MASS SPECTROMETRY; METABOLISM; MOLECULAR WEIGHT; OLIGOMERS; POLYMERASE CHAIN REACTION; PURIFICATION; UREA;

PROTEINS;

BACTERIAL ANTIGEN; PLASMID DNA; POLYMER; PROTEIN MPT 64; TETRAMER; UNCLASSIFIED DRUG; UREA;

ALPHA HELIX; ARTICLE; BACTERIAL STRAIN; BETA SHEET; CIRCULAR DICHROISM; CONTROLLED STUDY; DISULFIDE BOND; IMMUNITY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR CLONING; MOLECULAR WEIGHT; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN REFOLDING; WESTERN BLOTTING;

ANTIGENS, BACTERIAL; CIRCULAR DICHROISM; GENE EXPRESSION PROFILING; MOLECULAR WEIGHT; MYCOBACTERIUM TUBERCULOSIS; PLASMIDS; POLYMERASE CHAIN REACTION; PROTEIN REFOLDING; PROTEIN STRUCTURE, SECONDARY;

BACILLI (CLASS); MYCOBACTERIUM TUBERCULOSIS;

EID: 79959474984     PISSN: 08854513     EISSN: 14708744     Source Type: Journal    
DOI: 10.1002/bab.26     Document Type: Article

References (16)

1. Xing, Z., (2001) Curr. Pharm. Des. 7, 1015-1037.
2. Ortona, L., and, Antinori, A., (1998) Rays 23, 78-86.
3. Berthet, F. X., Rasmussen, P. B., Rosenkrands, I., Andersen, P., and, Gicquel, B., (1998) Microbiology 144, 3195-3203.
4. Roche, P. W., Triccas, J. A., Avery, D. T., Fifis, T., Billman-Jacobe, H., and, Britton, W. J., (1994) J. Infect. Dis. 170, 1326-1330.
5. Roche, P. W., Feng, C. G., and, Britton, W. J., (1996) Scand. J. Immunol. 43, 662-670.
6. Geisbrecht, B. V., Nikonenko, B., Samala, R., Nakamura, R., Nacy, C. A., and, Sacksteder, K. A., (2006) Protein Expr. Purif. 46, 64-72.
7. Andersen, P., Askgaard, D., Ljungqvist, L., Bennedsen, J., and, Heron, I., (1991) Infect. Immun. 59, 1905-1910.
8. Kalra, M., Grover, A., Mehta, N., Singh, J., Kaur, J., Sable, S. B., Behera, D., Sharma, P., Verma, I., and, Khuller, G. K., (2007) Clin. Immunol. 125, 173-183.
9. Zhu, D., Jiang, S., and, Luo, X., (2005) Vaccine 23, 4619-4624.
10. Bange, F. C., Collins, F. M., and, Jacobs, W. R. Jr., (1999) Tuberc. Lung. Dis. 79, 171-180.
11. Wang, Z., Potter, B. M., Gray, A. M., Sacksteder, K. A., Geisbrecht, B. V., and, Laity, J. H., (2007) J. Mol. Biol. 366, 375-381.
12. Papageorgiou, A. C., and, Acharya, K. R., (1997) Structure 5, 991-996.
13. Van Den, B. B., Tessari, M., Boelens, R., Dijkman, R., de Haas, G. H., Kaptein, R., and, Verheij, H. M., (1995) Nat. Struct. Biol. 2, 402-406.
14. Geisbrecht, B. V., Hamaoka, B. Y., Perman, B., Zemla, A., and, Leahy, D. J., (2005) J. Biol. Chem. 280, 17243-17250.
15. Harboe, M., Nagai, S., Patarroyo, M. E., Torres, M. L., Ramirez, C., and, Cruz, N., (1986) Infect. Immun. 52, 293-302.
16. Yamaguchi, R., Matsuo, K., Yamazaki, A., Abe, C., Nagai, S., Terasaka, K., and, Yamada, T., (1989) Infect. Immun. 57, 283-288.