Design and optimization of a recombinant system for large-scale production of the MPT64 antigen from Mycobacterium tuberculosis

Protein Expression and Purification

Volumn 46, Issue 1, 2006, Pages 64-72

  Geisbrecht, Brian V ^a   Nikonenko, Boris ^b   Samala, Rowena ^b   Nakamura, Reiko ^b   Nacy, Carol A ^b   Sacksteder, Katherine A ^b  

^a UNIVERSITY OF MISSOURI KANSAS CITY   (United States)

^b SEQUELLA INC   (United States)

Author keywords

Mycobacterium tuberculosis; PPD; PTAT; Tuberculosis; Tuberculosis diagnostic

Indexed keywords

BACTERIAL ANTIGEN; BACTERIAL DNA; MPT64 PROTEIN, MYCOBACTERIUM TUBERCULOSIS; PEPTIDE FRAGMENT; PRIMER DNA; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CHEMISTRY; ESCHERICHIA COLI; GENETICS; GUINEA PIG; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; MOLECULAR GENETICS; MYCOBACTERIUM TUBERCULOSIS; NUCLEOTIDE SEQUENCE; RESTRICTION MAPPING; SKIN TEST;

AMINO ACID SEQUENCE; ANIMALS; ANTIGENS, BACTERIAL; BASE SEQUENCE; DNA PRIMERS; DNA, BACTERIAL; ESCHERICHIA COLI; GUINEA PIGS; HUMANS; MOLECULAR SEQUENCE DATA; MYCOBACTERIUM TUBERCULOSIS; PEPTIDE FRAGMENTS; RECOMBINANT PROTEINS; RESTRICTION MAPPING; SKIN TESTS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

ANIMALIA; CAVIA PORCELLUS; ESCHERICHIA COLI; MYCOBACTERIUM BOVIS BCG; MYCOBACTERIUM TUBERCULOSIS;

EID: 32644456478     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.08.011     Document Type: Article

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