Vibrio cholerae requires the type VI secretion system virulence factor vasx to kill dictyostelium discoideum

Infection and Immunity

Volumn 79, Issue 7, 2011, Pages 2941-2949

  Miyata, Sarah T ^a   Kitaoka, Maya ^a   Brooks, Teresa M ^a   McAuley, Steven B ^a   Pukatzki, Stefan ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; MEMBRANE LIPID; TRANSLOCON; UNCLASSIFIED DRUG; VASH PROTEIN; VIRULENCE FACTOR; VIRULENCE FACTOR VASX;

ARTICLE; BACTERIAL MEMBRANE; BACTERIAL VIRULENCE; DICTYOSTELIUM DISCOIDEUM; GENE DELETION; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN SYNTHESIS; TYPE VI SECRETION SYSTEM; VIBRIO CHOLERAE;

BACTERIAL SECRETION SYSTEMS; DICTYOSTELIUM; GENE EXPRESSION REGULATION, BACTERIAL; MASS SPECTROMETRY; MEMBRANE LIPIDS; POLYMERASE CHAIN REACTION; PROTEIN STRUCTURE, TERTIARY; SEQUENCE DELETION; VIBRIO CHOLERAE; VIRULENCE FACTORS;

EID: 79959433450     PISSN: 00199567     EISSN: 10985522     Source Type: Journal    
DOI: 10.1128/IAI.01266-10     Document Type: Article

References (43)

1. Arnold, R. S., and R. B. Cornell. 1996. Lipid regulation of CTP: phosphocholine cytidylyltransferase: electrostatic, hydrophobic, and synergistic interactions of anionic phospholipids and diacylglycerol. Biochemistry 35:9917- 9924.
2. Aschtgen, M. S., C. S. Bernard, S. De Bentzmann, R. Lloubes, and E. Cascales. 2008. SciN is an outer membrane lipoprotein required for type VI secretion in enteroaggregative Escherichia coli. J. Bacteriol. 190:7523-7531.
3. Aubert, D. F., R. S. Flannagan, and M. A. Valvano. 2008. A novel sensor kinase-response regulator hybrid controls biofilm formation and type VI secretion system activity in Burkholderia cenocepacia. Infect. Immun. 76: 1979-1991.
4. Ballister, E. R., A. H. Lai, R. N. Zuckermann, Y. Cheng, and J. D. Mougous. 2008. In vitro self-assembly of tailorable nanotubes from a simple protein building block. Proc. Natl. Acad. Sci. U. S. A. 105:3733-3738.
5. Bennett-Lovsey, R. M., A. D. Herbert, M. J. Sternberg, and L. A. Kelley. 2008. Exploring the extremes of sequence/structure space with ensemble fold recognition in the program Phyre. Proteins 70:611-625.
6. Bernard, C. S., Y. R. Brunet, M. Gavioli, R. Lloubes, and E. Cascales. 4 March 2011. Regulation of type VI secretion gene clusters by {sigma}54 and cognate enhancer binding proteins. J. Bacteriol. doi:10.1128/JB.00029-11.
7. Bretscher, M. S. 1972. Asymmetrical lipid bilayer structure for biological membranes. Nat. New Biol. 236:11-12.
8. Broberg, C. A., L. Zhang, H. Gonzalez, M. A. Laskowski-Arce, and K. Orth. 2010. A Vibrio effector protein is an inositol phosphatase and disrupts host cell membrane integrity. Science 329:1660-1662.
9. Cosson, P., et al. 2002. Pseudomonas aeruginosa virulence analyzed in a Dictyostelium discoideum host system. J. Bacteriol. 184:3027-3033.
10. Dziejman, M., et al. 2002. Comparative genomic analysis of Vibrio cholerae: genes that correlate with cholera endemic and pandemic disease. Proc. Natl. Acad. Sci. U. S. A. 99:1556-1561.
11. Faruque, S. M., G. B. Nair, and J. J. Mekalanos. 2004. Genetics of stress adaptation and virulence in toxigenic Vibrio cholerae. DNA Cell Biol. 23: 723-41.
12. Filloux, A., A. Hachani, and S. Bleves. 2008. The bacterial type VI secretion machine: yet another player for protein transport across membranes. Microbiology 154:1570-1583.
13. Finkelstein, R. A., and J. J. LoSpalluto. 1969. Pathogenesis of experimental cholera. Preparation and isolation of choleragen and choleragenoid. J. Exp. Med. 30:185-202.
14. Gordesky, S. E., and G. V. Marinetti. 1973. The asymetric arrangement of phospholipids in the human erythrocyte membrane. Biochem. Biophys. Res. Commun. 50:1027-1031.
15. Guzman, L. M., D. Belin, M. J. Carson, and J. Beckwith. 1995. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J. Bacteriol. 177:4121-4130.
16. Hernandez, L. D., K. Hueffer, M. R. Wenk, and J. E. Galan. 2004. Salmonella modulates vesicular traffic by altering phosphoinositide metabolism. Science 304:1805-1807.
17. Herrington, D. A., et al. 1988. Toxin, toxin-coregulated pili, and the toxR regulon are essential for Vibrio cholerae pathogenesis in humans. J. Exp. Med. 68:1487-1492.
18. Hood, R. D., et al. 2010. A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria. Cell Host Microbe 7:25-37.
19. Kelley, L. A., and M. J. Sternberg. 2009. Protein structure prediction on the Web: a case study using the Phyre server. Nat. Protoc. 4:363-371.
20. Leiman, P. G., et al. 2009. Type VI secretion apparatus and phage tailassociated protein complexes share a common evolutionary origin. Proc. Natl. Acad. Sci. U. S. A. 106:4154-4159.
21. Lemmon, M. A. 2004. Pleckstrin homology domains: not just for phosphoinositides. Biochem. Soc. Trans. 32:707-711.
22. Lindquist, S. 1986. The heat-shock response. Annu. Rev. Biochem. 55:1151- 1191.
23. Livak, K. J., and T. D. Schmittgen. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 5:402-408.
24. Ma, A. T., S. McAuley, S. Pukatzki, and J. J. Mekalanos. 2009. Translocation of a Vibrio cholerae type VI secretion effector requires bacterial endocytosis by host cells. Cell Host Microbe 5:234-243.
25. Ma, L. S., J. S. Lin, and E. M. Lai. 2009. An IcmF family protein, ImpLM, is an integral inner membrane protein interacting with ImpKL, and its walker a motif is required for type VI secretion system-mediated Hcp secretion in Agrobacterium tumefaciens. J. Bacteriol. 191:4316-4329.
26. MacIntyre, D. L., S. T. Miyata, M. Kitaoka, and S. Pukatzki. 2010. The Vibrio cholerae type VI secretion system displays antimicrobial properties. Proc. Natl. Acad. Sci. U. S. A. 107:19520-19524.
27. Metcalf, W. W., et al. 1996. Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria. Plasmid 35:1-13.
28. Morita, Y. S., et al. 2010. Stress-induced synthesis of phosphatidylinositol 3-phosphate in mycobacteria. J. Biol. Chem. 285:16643-16650.
29. Mougous, J. D., et al. 2006. A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus. Science 312:1526-1530.
30. Mueller, R. S., S. Beyhan, S. G. Saini, F. H. Yildiz, and D. H. Bartlett. 2009. Indole acts as an extracellular cue regulating gene expression in Vibrio cholerae. J. Bacteriol. 191:3504-3516.
31. Nandi, B., R. K. Nandy, A. Sarkar, and A. C. Ghose. 2005. Structural features, properties and regulation of the outer-membrane protein W (OmpW) of Vibrio cholerae. Microbiology 151:2975-2986.
32. Niebuhr, K., et al. 2002. Conversion of PtdIns(4,5)P(2) into PtdIns(5)P by the S. flexneri effector IpgD reorganizes host cell morphology. EMBO J. 1:5069-5078.
33. Pukatzki, S., R. H. Kessin, and J. J. Mekalanos. 2002. The human pathogen Pseudomonas aeruginosa utilizes conserved virulence pathways to infect thesocial amoeba Dictyostelium discoideum. Proc. Natl. Acad. Sci. U. S. A. 99:3159-3164.
34. Pukatzki, S., A. T. Ma, A. T. Revel, D. Sturtevant, and J. J. Mekalanos. 2007. Type VI secretion system translocates a phage tail spike-like protein into target cells where it cross-links actin. Proc. Natl. Acad. Sci. U. S. A. 104: 15508-15513.
35. Pukatzki, S., et al. 2006. Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model system. Proc. Natl. Acad. Sci. U. S. A. 103:1528-1533.
36. Pukatzki, S., S. B. McAuley, and S. T. Miyata. 2009. The type VI secretion system: translocation of effectors and effector-domains. Curr. Opin. Microbiol. 2:11-17.
37. Sandkvist, M., L. P. Hough, M. M. Bagdasarian, and M. Bagdasarian. 1999. Direct interaction of the EpsL and EpsM proteins of the general secretion apparatus in Vibrio cholerae. J. Bacteriol. 181:3129-3135.
38. Schwarz, S., et al. 2010. Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 6:pii: 1001068.
39. Söding, J., A. Biegert, and A. N. Lupas. 2005. The HHpred interactive server for protein homology detection and structure prediction. Nucleic Acids Res. 3:W244-W248.
40. Solomon, J. M., A. Rupper, J. A. Cardelli, and R. R. Isberg. 2000. Intracellular growth of Legionella pneumophila in Dictyostelium discoideum, a system for genetic analysis of host-pathogen interactions. Infect. Immun. 68:2939- 2947.
41. Sussman, M. 1987. Cultivation and synchronous morphogenesis of Dictyostelium under controlled experimental conditions. Methods Cell Biol. 28:9- 29.
42. Wandler, A. M., R. Parthasarathy, and K. Guillemin. 2010. A greasy foothold for Helicobacter pylori. Cell Host Microbe 7:338-339.
43. Zinnaka, Y., and C. C. Carpenter, Jr. 1972. An enterotoxin produced by noncholera vibrios. Johns Hopkins Med. J. 131:403-411.