2-D zymographic analysis of Broccoli (Brassica oleracea L. var. Italica) florets proteases: Follow up of cysteine protease isotypes in the course of post-harvest senescence

Journal of Plant Physiology

Volumn 168, Issue 13, 2011, Pages 1517-1525

  Rossano, Rocco ^a   Larocca, Marilena ^a   Riccio, Paolo ^a  

^a UNIVERSITY OF BASILICATA   (Italy)

Author keywords

Broccoli; Post harvest; Proteases; Senescence; Zymography

Indexed keywords

CHLOROPHYLL; CYSTEINE PROTEINASE; ISOENZYME; PLANT EXTRACT; VEGETABLE PROTEIN;

ARTICLE; BRASSICA; CHEMISTRY; ENZYMOLOGY; FLOWER; GENE EXPRESSION REGULATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; ISOELECTRIC FOCUSING; METABOLISM; METHODOLOGY; PHOSPHORYLATION; PHYSIOLOGY; TIME; TWO DIMENSIONAL GEL ELECTROPHORESIS;

BRASSICA; CHLOROPHYLL; CYSTEINE PROTEASES; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; FLOWERS; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, PLANT; ISOELECTRIC FOCUSING; ISOENZYMES; PHOSPHORYLATION; PLANT EXTRACTS; PLANT PROTEINS; TIME FACTORS;

BRASSICA OLERACEA; BRASSICA OLERACEA VAR. CAPITATA; BRASSICA OLERACEA VAR. ITALICA;

EID: 79959376649     PISSN: 01761617     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jplph.2011.02.013     Document Type: Article

References (28)

1. Ames B.M., Shigena M.K., Hagen T.M. Oxidants, antioxidants and the degenarative diseases of aging. Proc Natl Acad Sci USA 1993, 90:7915-7922.
2. Beers E.P., Woffenden B.J., Zhao C.S. Plant proteolytic enzymes: possible roles during programmed cell death. Plant Mol Biol 2000, 44:399-415.
3. Bhattacharya R., Bhattacharya D. Resistance of bromelain to SDS binding. Biochem Biophys Acta 2009, 1794:698-708.
4. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976, 72:248-254.
5. Buchanan-Wollaston V. The molecular biology of leaf senescence. J Exp Bot 1997, 48:181-199.
6. Choi N.S., Yoo K.H., Yoon K.S., Maeng P.J., Kim S.H. Use of combined two dimensional fibrin zymography with SYPRO Ruby staining to identify the Bacillus sp. proteases. J Biochem Mol Biol 2004, 37:298-303.
7. Costa M.L., Civello P.M., Chaves A.R., Marti{dotless}nez G.A. Effect of hot air treatments on senescence and quality parameters of harvested broccoli (Brassica oleracea L. var. italica) heads. J Sci Food Agric 2005, 85:1154-1160.
8. Coupe S.A., Sinclair B.K., Watson L.M., Heyes J.A., Eason J.R. Identification of dehydration-responsive cysteine proteases during postharvest senescence of broccoli florets. J Exp Bot 2003, 54:1045-1056.
9. Delannoy M., Alves G., Vertommen D., Ma J., Boutry M., Navarre C. Identification of peptidases in Nicotiana tabacum leaf intercellular fluid. Proteomics 2008, 8:2285-2298.
10. Eason J.R., Ryan D.J., Watson L.M., Hedderley D., Christey M.C., Braun R.H., et al. Suppression of the cysteine protease, aleurain, delays floret senescence in Brassica oleracea. Plant Mol Biol 2005, 57:645-657.
11. Granell M., Cercos M., Carbonell J. Plant cysteine proteinases in germination and senescence. The Handbook of Proteolytic Enzymes 1998, 578-583. Academic Press, London. A.J. Barrett, N.D. Rawling, J.F. Woessner (Eds.).
12. Kaino S., Furui T., Datano S., Kaino M., Okita K., Nakamura K. Two-dimensional zymography for analysis of proteolytic enzymes in human pure pancreatic juice. Electrophoresis 1998, 19:782-787.
13. King G.A., Morris S.C. Physiological changes of broccoli during early postharvest senescence and through the preharvest-postharvest continuum. J Am Soc Hort Sci 1994, 119:270-275.
14. Kushad M.K., Brown A.F., Kurilich A.C., Juvik J.A., Klein B.P., Wallig M.A., et al. Variation of glucosinolates in vegetable crops of Brassica oleracea L. J Agric Food Chem 1999, 47:1541-1548.
15. Larocca M., Rossano R., Santamaria M., Riccio P. Analysis of pineapple [Ananas comosus (L.) Merr.] fruit proteinases by 2-D zymography and direct identification of the major zymographic spots by mass spectrometry. Food Chem 2010, 123:1334-1342.
16. Larocca M., Rossano R., Riccio P. Analysis of green kiwifruit (Actinidia deliciosa cv. Hayward) proteinases by 2D zymography and direct identification of zymographic spots by mass spectrometry. J Sci Food Agric 2010, 90:2411-2418.
17. Matile P., Hortensteiner S., Thomas H. Chlorophyll degradation. Annu Rev Plant Physiol Plant Mol Biol 1999, 50:67-95.
18. Page T., Griffiths G., Buchanan-Wollaston V. Molecular and biochemical characterization of postharvest senescence in broccoli. Plant Physiol 2001, 125:718-727.
19. Porra R.J., Thompson W.A., Kriedmann P.E. Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochem Biophys Acta 1989, 975:384-394.
20. Pucci-Minafra I., Minafra S., La Rocca G., Barranca M., Fontana S., Alaimo G., et al. Zymographic analysis of circulating and tissue forms of colon carcinoma gelatinase A (MMP-2) and B (MMP-9) separated by mono- and two-dimensional electrophoresis. Matrix Biol 2001, 20:419-427.
21. Rice-Evans C., Miller N.J., Botwell P.G., Bramley P.M., Pridham J.B. The relative antioxidant activities of plant derived polyphenolic flavonoids. Free Radical Res 1994, 22:375-383.
22. Saitoh E., Yamamoto S., Okamoto E., Hayakawa Y., Hoshino T., Sato R., et al. Identification of cysteine proteases and screening of cysteine protease inhibitors in biological samples by a two-dimensional gel system of zymography and reverse zymography. Anal Chem Insights 2007, 2:51-59.
23. Smart C.M. Gene expression during leaf senescence. New Phytol 1994, 126:419-448.
24. Stephenson P., Rubinstein B. Characterization of proteolytic activity during senescence in daylilies. Physiol Plant 1998, 104:463-473.
25. Stoner G., Morse M.A. Isothiocyanates and plant polyphenols as inhibitors of lung and esophaegeal cancer. Cancer Lett 1997, 114:113-119.
26. Valpuesta V., Lange N.E., Guerrero C., Reid M.S. Up-regulation of a cysteine protease accompanies the ethylene-insensitive senescence of daylily (Hemerocallis) flowers. Plant Mol Biol 1995, 28:575-582.
27. Wagstaff C., Leverentz M.K., Griffiths G., Thomas B., Chanasut U., Stead A.D., et al. Cysteine protease gene expression and proteolytic activity during senescence of Alstroemeria petals. J Exp Bot 2002, 53:233-240.
28. Wang Y.T., Yang C.Y., Chen Y.T., Lin Y., Shaw J.F. Characterization of senescence-associated proteases in postharvest broccoli florets. Plant Physiol Biochem 2004, 42:663-670.