Roles of cysteine proteases Cwp84 and Cwp13 in biogenesis of the cell wall of Clostridium difficile

Journal of Bacteriology

Volumn 193, Issue 13, 2011, Pages 3276-3285

  de la Riva, Lucía ^a   Willing, Stephanie E ^a   Tate, Edward W ^a   Fairweather, Neil F ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CELL MEMBRANE PROTEIN; CYSTEINE PROTEINASE;

ARTICLE; BACTERIAL CELL WALL; BACTERIAL MUTATION; BACTERIAL STRAIN; BACTERIUM COLONY; BACTERIUM MUTANT; BIOGENESIS; CATALYSIS; CELL SURFACE; CLOSTRIDIUM DIFFICILE; CONTROLLED STUDY; CULTURE MEDIUM; GENETIC COMPLEMENTATION; NONHUMAN; PLASMID; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; CELL WALL; CLOSTRIDIUM DIFFICILE; CYSTEINE ENDOPEPTIDASES; GENE KNOCKOUT TECHNIQUES; GENETIC COMPLEMENTATION TEST; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL; MUTANT PROTEINS; PROTEIN PROCESSING, POST-TRANSLATIONAL;

CLOSTRIDIUM DIFFICILE;

EID: 79959365481     PISSN: 00219193     EISSN: 10985530     Source Type: Journal    
DOI: 10.1128/JB.00248-11     Document Type: Article

References (42)

1. Bartlett, J. G. 2007. Clostridium difficile: old and new observations. J. Clin. Gastroenterol. 41(Suppl. 1):S24-S29.
2. Bertani, G. 2004. Lysogeny at mid-twentieth century: P1, P2, and other experimental systems. J. Bacteriol. 186:595-600.
3. Brannigan, J. A., et al. 1995. A protein catalytic framework with an N-terminal nucleophile is capable of self-activation. Nature 378:416-419.
4. Calabi, E., and N. Fairweather. 2002. Patterns of sequence conservation in the S-layer proteins and related sequences in Clostridium difficile. J. Bacteriol. 184:3886-3897.
5. Calabi, E., et al. 2001. Molecular characterization of the surface layer proteins from Clostridium difficile. Mol. Microbiol. 40:1187-1199.
6. Cappetta, M., I. Roth, A. Diaz, J. Tort, and L. Roche. 2002. Role of the prosegment of Fasciola hepatica cathepsin L1 in folding of the catalytic domain. Biol. Chem. 383:1215-1221.
7. Chen, R., and U. Henning. 1996. A periplasmic protein (Skp) of Escherichia coli selectively binds a class of outer membrane proteins. Mol. Microbiol. 19:1287-1294.
8. Cloud, J., and C. P. Kelly. 2007. Update on Clostridium difficile associated disease. Curr. Opin. Gastroenterol. 23:4-9.
9. Dang, T., et al. 2010. Chemical probes of surface layer biogenesis in Clostridium difficile. ACS Chem. Biol. 5:279-285.
10. Dautin, N., T. J. Barnard, D. E. Anderson, and H. D. Bernstein. 2007. Cleavage of a bacterial autotransporter by an evolutionarily convergent autocatalytic mechanism. EMBO J. 26:1942-1952.
11. Emerson, J., and N. F. Fairweather. 2009. Surface structures of Clostridium difficile and other clostridia, p. 157-167. In H. Bruggemann and G. Gottschalk (ed.), Clostridia: molecular biology in the post-genomic era. Caister Academic Press, Norfolk, United Kingdom.
12. Emerson, J., et al. 2009. A novel genetic switch controls phase variable expression of CwpV, a Clostridium difficile cell wall protein. Mol. Microbiol. 74:541-556.
13. Fagan, R., and N. Fairweather. 2010. Dissecting the cell surface, p. 117-134. In P. Mullany and A. P. Roberts (ed.), Methods Mol. Biol, 2010/07/03 ed., vol. 646. Humana Press, Inc., New York, NY.
14. Fagan, R. P., et al. 2009. Structural insights into the molecular organization of the S-layer from Clostridium difficile. Mol. Microbiol. 71:1308-1322.
15. Fox, T., E. de Miguel, J. S. Mort, and A. C. Storer. 1992. Potent slow-binding inhibition of cathepsin B by its propeptide. Biochemistry 31:12571-12576.
16. Freeman, J., et al. 2010. The changing epidemiology of Clostridium difficile infections. Clin. Microbiol. Rev. 23:529-549.
17. Heap, J. T., O. J. Pennington, S. T. Cartman, G. P. Carter, and N. P. Minton. 2007. The ClosTron: a universal gene knockout system for the genus Clostridium. J. Microbiol. Methods 70:452-464.
18. Heap, J. T., O. J. Pennington, S. T. Cartman, and N. P. Minton. 2009. A modular system for Clostridium shuttle plasmids. J. Microbiol. Methods 78:79-85.
19. Hussain, H. A., A. P. Roberts, and P. Mullany. 2005. Generation of an erythromycin-sensitive derivative of Clostridium difficile strain 630 (630Δerm) and demonstration that the conjugative transposon Tn916E enters the genome of this strain at multiple sites. J. Med. Microbiol. 54:137-141.
20. Janoir, C., S. Pechine, C. Grosdidier, and A. Collignon. 2007. Cwp84, a surface-associated protein of Clostridium difficile, is a cysteine protease with degrading activity on extracellular matrix proteins. J. Bacteriol. 189:7174-7180.
21. Just, I., and R. Gerhard. 2004. Large clostridial cytotoxins. Rev. Physiol. Biochem. Pharmacol. 152:23-47.
22. Karjalainen, T., et al. 2001. Molecular and genomic analysis of genes encoding surface-anchored proteins from Clostridium difficile. Infect. Immun. 69:3442-3446.
23. Kirby, J. M., et al. 2009. Cwp84, a surface-associated cysteine protease, plays a role in the maturation of the surface layer of Clostridium difficile. J. Biol. Chem. 284:34666-34673.
24. Kuehne, S. A., et al. 2010. The role of toxin A and toxin B in Clostridium difficile infection. Nature 467:711-713.
25. Lawley, T. D., et al. 2009. Antibiotic treatment of Clostridium difficile carrier mice triggers a supershedder state, spore-mediated transmission, and severe disease in immunocompromised hosts. Infect. Immun. 77:3661-3669.
26. Lemee, L., et al. 2005. Multilocus sequence analysis and comparative evolution of virulence-associated genes and housekeeping genes of Clostridium difficile. Microbiology 151:3171-3180.
27. Lyras, D., et al. 2009. Toxin B is essential for virulence of Clostridium difficile. Nature 458:1176-1179.
28. Matias, V. R., and T. J. Beveridge. 2005. Cryo-electron microscopy reveals native polymeric cell wall structure in Bacillus subtilis 168 and the existence of a periplasmic space. Mol. Microbiol. 56:240-251.
29. Nagler, D. K., et al. 1999. Human cathepsin X: a cysteine protease with unique carboxypeptidase activity. Biochemistry 38:12648-12654.
30. Pechine, S., et al. 2005. Immunological properties of surface proteins of Clostridium difficile. J. Med. Microbiol. 54:193-196.
31. Poxton, I. R., and T. D. Cartmill. 1982. Immunochemistry of the cell-surface carbohydrate antigens of Clostridium difficile. J. Gen. Microbiol. 128:1365-1370.
32. Purdy, D., et al. 2002. Conjugative transfer of clostridial shuttle vectors from Escherichia coli to Clostridium difficile through circumvention of the restriction barrier. Mol. Microbiol. 46:439-452.
33. Rawlings, N. D., A. J. Barrett, and A. Bateman. 2010. MEROPS: the peptidase database. Nucleic Acids Res. 38:D227-D233.
34. Rupnik, M., M. H. Wilcox, and D. N. Gerding. 2009. Clostridium difficile infection: new developments in epidemiology and pathogenesis. Nat. Rev. Microbiol. 7:526-536.
35. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual. Cold Spring Harbor Press, Cold Spring Harbor, NY.
36. Sarvas, M., C. R. Harwood, S. Bron, and J. M. van Dijl. 2004. Post-translocational folding of secretory proteins in Gram-positive bacteria. Biochim. Biophys. Acta 1694:311-327.
37. Savariau-Lacomme, M. P., C. Lebarbier, T. Karjalainen, A. Collignon, and C. Janoir. 2003. Transcription and analysis of polymorphism in a cluster of genes encoding surface-associated proteins of Clostridium difficile. J. Bacteriol. 185:4461-4470.
38. Sklar, J. G., T. Wu, D. Kahne, and T. J. Silhavy. 2007. Defining the roles of the periplasmic chaperones SurA, Skp, and DegP in Escherichia coli. Genes Dev. 21:2473-2484.
39. Strauch, K. L., K. Johnson, and J. Beckwith. 1989. Characterization of degP, a gene required for proteolysis in the cell envelope and essential for growth of Escherichia coli at high temperature. J. Bacteriol. 171:2689-2696.
40. Tormo, A., M. Almiron, and R. Kolter. 1990. surA, an Escherichia coli gene essential for survival in stationary phase. J. Bacteriol. 172:4339-4347.
41. Waligora, A. J., et al. 2001. Characterization of a cell surface protein of Clostridium difficile with adhesive properties. Infect. Immun. 69:2144-2153.
42. Wiederanders, B. 2003. Structure-function relationships in class CA1 cysteine peptidase propeptides. Acta Biochim. Pol. 50:691-713.