Genetic transformation of the relapsing fever spirochete Borrelia hermsii: Stable integration and expression of green fluorescent protein from linear plasmid 200

Journal of Bacteriology

Volumn 193, Issue 13, 2011, Pages 3241-3245

  Fine, Lindy M ^a   Earnhart, Christopher G ^a   Marconi, Richard T ^a  

^a VIRGINIA COMMONWEALTH UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN;

ARTICLE; BACTERIAL GENOME; BACTERIAL STRAIN; BACTERIAL TRANSMISSION; BACTERIAL VIRULENCE; BORRELIA HERMSII; BORRELIA INFECTION; GENE SEQUENCE; GENETIC MANIPULATION; GENETIC TRANSFORMATION; HUMAN; IN VITRO STUDY; IN VIVO STUDY; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; SEROCONVERSION; TICK BORNE RELAPSING FEVER;

ANIMALS; ANTIBODIES, BACTERIAL; BORRELIA; DISEASE MODELS, ANIMAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION; GENOMIC INSTABILITY; GREEN FLUORESCENT PROTEINS; MICE; MOLECULAR BIOLOGY; MUTAGENESIS, INSERTIONAL; PLASMIDS; RECOMBINATION, GENETIC; RELAPSING FEVER; TRANSFORMATION, BACTERIAL; VIRULENCE;

BORRELIA; BORRELIA HERMSII; BORRELIA TURICATAE; IXODIDA; ORNITHODOROS; SPIROCHAETALES;

EID: 79959358437     PISSN: 00219193     EISSN: 10985530     Source Type: Journal    
DOI: 10.1128/JB.05037-11     Document Type: Article

References (35)

1. Barbour, A. G., Q. Dai, B. I. Restrepo, H. G. Stoenner, and S. A. Frank. 2006. Pathogen escape from host immunity by a genome program for antigenic variation. Proc. Natl. Acad. Sci. U. S. A. 103:18290-18295.
2. Battisti, J. M., S. J. Raffel, and T. G. Schwan. 2008. A system for site-specific genetic manipulation of the relapsing fever spirochete Borrelia hermsii. Methods Mol. Biol. 431:69-84.
3. Boyer, K. M., et al. 1977. Tick-borne relapsing fever: an interstate outbreak originating at Grand Canyon National Park. Am. J. Epidemiol. 105:469-479.
4. Carlyon, J. A., C. LaVoie, S. Y. Sung, and R. T. Marconi. 1998. Analysis of the organization of multicopy linear-and circular-plasmid-carried open reading frames in Borrelia burgdorferi sensu lato isolates. Infect. Immun. 66:1149-1158.
5. Cutler, S., and A. Talbert. 2003. Tick-borne relapsing fever in Tanzania-a forgotten problem? ASM News 69:542-543.
6. Dai, Q., et al. 2006. Antigenic variation by Borrelia hermsii occurs through recombination between extragenic repetitive elements on linear plasmids. Mol. Microbiol. 60:1329-1343.
7. Dupont, H. T., B. La Scola, R. Williams, and D. Raoult. 1997. A focus of tick-borne relapsing fever in southern Zaire. Clin. Infect. Dis. 25:139-144.
8. Dworkin, M., P. Shoemaker, C. Fritz, M. Dowell, and D. E. Anderson, Jr. 2002. The epidemiology of tick-borne relapsing fever in the United States. Am. J. Trop. Med. Hyg. 66:753-758.
9. Earnhart, C. G., et al. 2010. Identification of residues within ligand-binding domain 1 (LBD1) of the Borrelia burgdorferi OspC protein required for function in the mammalian environment. Mol. Microbiol. 76:393-408.
10. Eggers, C. H., M. J. Caimano, and J. D. Radolf. 2004. Analysis of promoter elements involved in the transcriptional initiation of RpoS-dependent Borrelia burgdorferi genes. J. Bacteriol. 186:7390-7402.
11. Felsenfeld, O. 1971. Borrelia. Strains, vectors, human and animal borreliosis. Warren H. Green, Inc., St. Louis, MO.
12. Fritz, C. L., et al. 2004. Isolation and characterization of Borrelia hermsii associated with two foci of tick-borne relapsing fever in California. J. Clin. Microbiol. 42:1123-1128.
13. Goubau, P. F. 1984. Relapsing fevers. A review. Ann. Soc. Belg. Med. Trop. 64:335-364.
14. Hovis, K. M., J. C. Freedman, H. Zhang, J. L. Forbes, and R. T. Marconi. 2008. Identification of an antiparallel coiled-coil/loop domain required for ligand binding by the Borrelia hermsii FhbA protein: additional evidence for the role of FhbA in the host-pathogen interaction. Infect. Immun. 76:2113-2122.
15. Hovis, K. M., J. V. McDowell, L. Griffin, and R. T. Marconi. 2004. Identification and characterization of a linear-plasmid-encoded factor H-binding protein (FhbA) of the relapsing fever spirochete Borrelia hermsii. J. Bacteriol. 186:2612-2618.
16. Hovis, K. M., M. E. Schriefer, S. Bahlani, and R. T. Marconi. 2006. Immunological and molecular analyses of the Borrelia hermsii factor H and factor H-like protein 1 binding protein, FhbA: demonstration of its utility as a diagnostic marker and epidemiological tool for tick-borne relapsing fever. Infect. Immun. 74:4519-4529.
17. Ilsley, M. L. 1952. Relapsing fever probably caused by Borrelia duttonii. Calif. Med. 77:195-196.
18. Jongen, V. H., J. van Roosmalen, J. Tiems, J. Van Holten, and J. C. Wetsteyn. 1997. Tick-borne relapsing fever and pregnancy outcome in rural Tanzania. Acta Obstet. Gynecol. Scand. 76:834-838.
19. Labandeira-Rey, M., and J. T. Skare. 2001. Decreased infectivity in Borrelia burgdorferi strain B31 is associated with the loss of either linear plasmid 25 or 28-1. Infect. Immun. 69:446-455.
20. Lawrenz, M. B., H. Kawabata, J. E. Purser, and S. J. Norris. 2002. Decreased electroporation efficiency in Borrelia burgdorferi containing linear plasmids lp25 and lp56: impact on transformation of infectious B. burgdorferi. Infect. Immun. 70:4798-4804.
21. Lescot, M., et al. 2008. The genome of Borrelia recurrentis, the agent of deadly louse-borne relapsing fever, is a degraded subset of tick-borne Borrelia duttonii. PLoS Genet. 4:e1000185.
22. Lopez, J. E., et al. 2008. Relapsing fever spirochetes retain infectivity after prolonged in vitro cultivation. Vector Borne Zoonotic Dis. 8:813-820.
23. Marconi, R. T., M. E. Konkel, and C. F. Garon. 1993. Variability of osp genes and gene products among species of Lyme disease spirochetes. Infect. Immun. 61:2611-2617.
24. McDowell, J. V., S. Y. Sung, M. Labandeira-Rey, J. T. Skare, and R. T. Marconi. 2001. Analysis of mechanisms associated with loss of infectivity of clonal populations of Borrelia burgdorferi B31MI. Infect. Immun. 69:3670-3677.
25. Purser, J. E., and S. J. Norris. 2000. Correlation between plasmid content and infectivity in Borrelia burgdorferi. Proc. Natl. Acad. Sci. U. S. A. 97: 13865-13870.
26. Ras, N., et al. 1996. Phylogenesis of relapsing fever Borrelia spp. Int. J. Syst. Bacteriol. 46:859-865.
27. Rebaudet, S., and P. Parola. 2006. Epidemiology of relapsing fever borreliosis in Europe. FEMS Immunol. Med. Microbiol. 48:11-15.
28. Rogers, E. A., et al. 2009. Rrp1, a cyclic-di-GMP-producing response regulator, is an important regulator of Borrelia burgdorferi core cellular functions. Mol. Microbiol. 71:1551-1573.
29. Rosa, P. A., K. Tilly, and P. E. Stewart. 2005. The burgeoning molecular genetics of the Lyme disease spirochaete. Nat. Rev. Microbiol. 3:129-143.
30. Samuels, D. S., K. Mach, and C. F. Garon. 1994. Genetic transformation of the Lyme disease agent Borrelia burgdorferi with coumarin-resistant gyrB. J. Bacteriol. 176:6045-6049.
31. Schwan, T. G., et al. 2003. Tick-borne relapsing fever caused by Borrelia hermsii in Montana. Emerg. Infect. Dis. 9:1151-1154.
32. Schwan, T. G., S. J. Raffel, M. E. Schrumpf, and S. F. Porcella. 2007. Diversity and distribution of Borrelia hermsii. Emerg. Infect. Dis. 13:436-442.
33. Schwan, T. G., et al. 2009. Tick-borne relapsing fever and Borrelia hermsii, Los Angeles County, California, USA. Emerg. Infect. Dis. 15:1026-1031.
34. Trevejo, R. T., et al. 1998. An interstate outbreak of tick-borne relapsing fever among vacationers at a rocky mountain cabin. Am. J. Trop. Med. Hyg. 58:743-747.
35. Vial, L., et al. 2006. Incidence of tick-borne relapsing fever in west Africa: longitudinal study. Lancet 368:37-43.