A novel and practical method using HRP-polymer conjugate and microwave treatment for visualization of 2 antigens raised from the same or different species in paraffin-embedded tissues

Applied Immunohistochemistry and Molecular Morphology

Volumn 19, Issue 4, 2011, Pages 376-383

  Chan, Anthony ^a   Matias, Marie Anne Teresa ^a   Farah, Camile Selim ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

double staining; HPR polymer conjugate; immunohistochemistry; microwave

Indexed keywords

ANTIGEN; FORMALDEHYDE; HORSERADISH PEROXIDASE; PARAFFIN;

ANIMAL EXPERIMENT; ANTIGEN DETECTION; ARTICLE; CLINICAL PROTOCOL; CONJUGATION; IMAGING SYSTEM; MICROWAVE THERAPY; NONHUMAN; PRIORITY JOURNAL; RAT; SPECIES DIFFERENCE; STAINING; TISSUE SECTION;

ANIMALS; ANTIGENS; BLOOD VESSELS; DIAGNOSTIC IMAGING; HORSERADISH PEROXIDASE; IMMUNOHISTOCHEMISTRY; MICE; MICROWAVES; PARAFFIN EMBEDDING; POLYMERS; RABBITS; RATS; RIBS; SPECIES SPECIFICITY; SPINAL CORD;

EID: 79959317536     PISSN: 15412016     EISSN: 15334058     Source Type: Journal    
DOI: 10.1097/PAI.0b013e31820251c0     Document Type: Article

References (10)

1. Nakane PK. Simultaneous localization of multiple tissue antigens using the peroxidase-labeled antibody method: a study on pituitary glands of the rat. J Histochem Cytochem. 1968;16:557-560.
2. Canese MG, Bussolati G. Immuno-electron-cytochemical localization of the somatostatin cells in the human antral mucosa. J Histochem Cytochem. 1977;25:1111-1118. (Pubitemid 8208661)
3. Lechago J, Sun NC, Weinstein WM. Simultaneous visualization of two antigens in the same tissue section by combining immuno peroxidase with immunofluorescence techniques. J Histochem Cytochem. 1979;27:1221-1225. (Pubitemid 10224077)
4. Glass G, Papin JA, Mandell JW. SIMPLE: a sequential immunoperoxidase labeling and erasing method. J Histochem Cytochem. 2009;57:899-905.
5. Kolodziejczyk E, Baertschi AJ. Multiple immunolabeling in histology: a new method using thermo-inactivation of immunoglobulins. J Histochem Cytochem. 1986;34:1725-1729. (Pubitemid 17177629)
6. Munoz TE, Giberson RT, Demaree R, et al. Microwave-assisted immunostaining: a new approach yields fast and consistent results. J Neurosci Methods. 2004;137:133-139. (Pubitemid 38942873)
7. Toth ZE, Mezey E, Toth ZE, et al. Simultaneous visualization of multiple antigens with tyramide signal amplification using antibodies from the same species. J Histochem Cytochem. 2007;55:545-554. (Pubitemid 46791937)
8. Gao C, Wang AY, Han YJ. Microwave antigen retrieval blocks endogenous peroxidase activity in immunohistochemistry. Appl Immunohistochem Mol Morphol. 2008;16:393-399.
9. Hunyady B, Krempels K, Harta G, et al. Immunohistochemical signal amplification by catalyzed reporter deposition and its application in double immunostaining. J Histochem Cytochem. 1996;44:1353-1362.
10. Van der Loos CM, van der Loos CM. Multiple immunoenzyme staining: methods and visualizations for the observation with spectral imaging. J Histochem Cytochem. 2008;56:313-328.