Cation permeation through connexin 43 hemichannels is cooperative, competitive and saturable with parameters depending on the permeant species

Biochemical and Biophysical Research Communications

Volumn 409, Issue 4, 2011, Pages 603-609

  Orellana, Juan A ^a   Díaz, Emilio ^a   Schalper, Kurt A ^a   Vargas, Aníbal A ^a   Bennett, Michael V L ^b   Sáez, Juan C ^a,b  

^a PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE   (Chile)

^b ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

Affinity constants; Connexons; Hill coefficient; Maximal transport capacity

Indexed keywords

4',6 DIAMIDINO 2 PHENYLINDOLE; CATION; CONNEXIN 43; DYE; ETHIDIUM; GREEN FLUORESCENT PROTEIN; NUCLEIC ACID; PROPIDIUM IODIDE;

ANIMAL CELL; ARTICLE; BINDING SITE; CATION TRANSPORT; CELL CULTURE; CONFOCAL MICROSCOPY; CONTROLLED STUDY; DIFFUSION; FEMALE; HELA CELL; HUMAN; HUMAN CELL; MEMBRANE PERMEABILITY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ANIMALS; CATIONS; CELL MEMBRANE PERMEABILITY; CONNEXIN 43; ETHIDIUM; FLUORESCENT DYES; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; INDOLES; MICE; PROPIDIUM;

EID: 79959219603     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2011.05.031     Document Type: Article

References (19)

1. Sohl G., Willecke K. Gap junctions and the connexin protein family. Cardiovasc. Res. 2004, 62:228-232.
2. Schalper K.A., Palacios-Prado N., Orellana J.A., Sáez J.C. Currently used methods for identification and characterization of hemichannels. Cell Commun. Adhes. 2008, 15:207-218.
3. Orellana J.A., Sáez P.J., Shoji K.F., Schalper K.A., Palacios-Prado N., Velarde V., Giaume C., Bennett M.V., Sáez J.C. Modulation of brain hemichannels and gap junction channels by pro-inflammatory agents and their possible role in neurodegeneration. Antioxid. Redox. Signal 2009, 11:369-399.
4. Retamal M.A., Froger N., Palacios-Prado N., Ezan P., Sáez P.J., Sáez J.C., Giaume C. Cx43 hemichannels and gap junction channels in astrocytes are regulated oppositely by proinflammatory cytokines released from activated microglia. J. Neurosci. 2007, 27:13781-13792.
5. Rana S., Dringen R. Gap junction hemichannel-mediated release of glutathione from cultured rat astrocytes. Neurosci. Lett. 2007, 415:45-48.
6. Ahmad S., Evans W.H. Gap junction hemichannel-mediated release of glutathione from cultured rat astrocytes. Biochem. J. 2002, 365:693-699.
7. Bruzzone S., Guida L., Zocchi E., Franco L., De Flora A. Connexin 43 hemi channels mediate Ca2+-regulated transmembrane NAD+ fluxes in intact cells. FASEB J. 2001, 15:10-12.
8. Contreras J.E., Sáez J.C., Bukauskas F.F., Bennett M.V. Gating and regulation of connexin 43 (Cx43) hemichannels. Proc. Natl. Acad. Sci. U.S.A. 2003, 100:11388-11393.
9. Schalper K.A., Palacios-Prado N., Retamal M.A., Shoji K.F., Martínez A.D., Sáez J.C. Connexin hemichannel composition determines the FGF-1-induced membrane permeability and free [Ca2+]i responses. Mol. Biol. Cell. 2008, 19:3501-3513.
10. Bukauskas F.F., Kreuzberg M.M., Rackauskas M., Bukauskiene A., Bennett M.V., Verselis V.K., Willecke K. Properties of mouse connexin 30.2 and human connexin 31.9 hemichannels: implications for atrioventricular conduction in the heart. Proc. Natl. Acad. Sci. U.S.A. 2006, 103:9726-9731.
11. Valiunas V. Biophysical properties of connexin-45 gap junction hemichannels studied in vertebrate cells. J. Gen. Physiol. 2002, 119:147-164.
12. Oh S., Verselis V.K., Bargiello T.A. Charges dispersed over the permeation pathway determine the charge selectivity and conductance of a Cx32 chimeric hemichannel. J. Physiol. 2008, 586:2445-2461.
13. Elfgang C., Eckert R., Lichtenberg-Frate H., Butterweck A., Traub O., Klein R.A., Hulser D.F., Willecke K. Specific permeability and selective formation of gap junction channels in connexin-transfected HeLa cells. J. Cell. Biol. 1995, 129:805-817.
14. Ebihara L., Liu X., Pal J.D. Effect of external magnesium and calcium on human connexin46 hemichannels. Biophys. J. 2003, 84:277-286.
15. Gómez-Hernández J.M., de Miguel M., Larrosa B., Gonzalez D., Barrio L.C. Molecular basis of calcium regulation in connexin-32 hemichannels. Proc. Natl. Acad. Sci. U.S.A. 2003, 100:16030-16035.
16. Bukauskas F.F., Bukauskiene A., Verselis V.K. Conductance and permeability of the residual state of connexin43 gap junction channels. J. Gen. Physiol. 2002, 119:171-185.
17. Qu Y., Dahl G. Function of the voltage gate of gap junction channels: selective exclusion of molecules. Proc. Natl. Acad. Sci. U.S.A. 2002, 99:697-702.
18. Nicholson B., Dermietzel R., Teplow D., Traub O., Willecke K., Revel J.P. Two homologous protein components of hepatic gap junctions. Nature 1987, 329:732-734.
19. Lin M.C., Huang Y.L., Liu H.W., Yang D.Y., Lee C.P., Yang L.L., Cheng F.C. On-line microdialysis-graphite furnace atomic absorption spectrometry in the determination of brain magnesium levels in gerbils subjected to cerebral ischemia/reperfusion. J. Am. Coll. Nutr. 2004, 23:561S-565S.