Determination of real-time efflux phenotypes in Escherichia coli AcrB binding pocket phenylalanine mutants using a 1,2′-dinaphthylamine efflux assay

PLoS ONE

Volumn 6, Issue 6, 2011, Pages

  Bohnert, Jürgen A ^a   Schuster, Sabine ^a   Szymaniak Vits, Magdalena ^a   Kern, Winfried V ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

1,2' DINAPHTHYLAMINE; AMINE; ARGININE; BACTERIAL PROTEIN; PHENYLALANINE; PROTEIN ACRB; UNCLASSIFIED DRUG; ACRE PROTEIN, E COLI; ESCHERICHIA COLI PROTEIN; MULTIDRUG RESISTANCE PROTEIN;

ARTICLE; BACTERIAL STRAIN; BINDING AFFINITY; BINDING SITE; BROTH DILUTION; CONTROLLED STUDY; ENZYMATIC ASSAY; ESCHERICHIA COLI; HYDROPHILICITY; LIPOPHILICITY; NONHUMAN; PHENOTYPE; POINT MUTATION; PROTEIN NUCLEIC ACID INTERACTION; RESIDUE ANALYSIS; SITE DIRECTED MUTAGENESIS; GENETICS; METABOLISM; MOLECULAR PROBE; PROTEIN BINDING;

ESCHERICHIA COLI;

ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MOLECULAR PROBES; MULTIDRUG RESISTANCE-ASSOCIATED PROTEINS; PHENYLALANINE; POINT MUTATION; PROTEIN BINDING;

EID: 79958858484     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0021196     Document Type: Article

References (13)

1. Nikaido H, (1998) Antibiotic resistance caused by gram-negative multidrug efflux pumps. Clin Infect Dis 27 (Suppl 1): S32-S41.
2. Piddock LJ, (2006) Clinically relevant chromosomally encoded multidrug resistance efflux pumps in bacteria. Clin Microbiol Rev 19: 382-402.
3. Murakami S, Nakashima R, Yamashita E, Matsumoto T, Yamaguchi A, (2006) Crystal structures of a multidrug transporter reveal a functionally rotating mechanism. Nature 443: 173-179.
4. Seeger MA, Schiefner A, Eicher T, Verrey F, Diederichs K, et al. (2006) Structural asymmetry of AcrB trimer suggests a peristaltic pump mechanism. Science 313: 1295-1298.
5. Sennhauser G, Amstutz P, Briand C, Storchenegger O, Grutter MG, (2006) Drug export pathway of multidrug exporter AcrB revealed by DARPin inhibitors. PLoS Biol 5: e7.
6. Mao W, Warren MS, Black DS, Satou T, Murata T, et al. (2002) On the mechanism of substrate specificity by resistance nodulation division (RND)-type multidrug resistance pumps: the large periplasmic loops of MexD from Pseudomonas aeruginosa are involved in substrate recognition. Mol Microbiol 46: 889-901.
7. Bohnert JA, Schuster S, Fähnrich E, Trittler R, Kern WV, (2007) Altered spectrum of multidrug resistance associated with a single point mutation in the Escherichia coli RND-type MDR efflux pump YhiV (MdtF). J Antimicrob Chemother 59: 1216-1222.
8. Bohnert JA, Schuster S, Seeger MA, Fähnrich E, Pos KM, et al. (2008) Site-directed mutagenesis reveals putative substrate binding residues in the Escherichia coli RND efflux pump AcrB. J Bacteriol 190: 8225-8229.
9. Nagano K, Nikaido H, (2009) Kinetic behavior of the major multidrug efflux pump AcrB of Escherichia coli. Proc Natl Acad Sci U S A 106: 5854-5858.
10. Bohnert JA, Karamian B, Nikaido H, (2010) Optimized Nile Red efflux assay of AcrAB-TolC multidrug efflux system shows competition between substrates. Antimicrob Agents Chemother 54: 3770-3775.
11. Jellen-Ritter AS, Kern WV, (2001) Enhanced expression of the multidrug efflux pumps AcrAB and AcrEF associated with insertion element transposition in Escherichia coli mutants Selected with a fluoroquinolone. Antimicrob Agents Chemother 45: 1467-1472.
12. Lomovskaya O, Warren MS, Lee A, Galazzo J, Fronko R, et al. (2001) Identification and characterization of inhibitors of multidrug resistance efflux pumps in Pseudomonas aeruginosa: novel agents for combination therapy. Antimicrob Agents Chemother 45: 105-116.
13. Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, et al. (2006) Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing. J Biomed Opt 11: 050507.