Cross-linking of lipases adsorbed on hydrophobic supports: Highly selective hydrolysis of fish oil catalyzed by RML

JAOCS, Journal of the American Oil Chemists' Society

Volumn 88, Issue 6, 2011, Pages 801-807

  Fernandez Lorente, Gloria ^a   Filice, Marco ^b   Lopez Vela, Dolores ^b   Pizarro, Carolina ^b,c   Wilson, Lorena ^c   Betancor, Lorena ^d   Avila, Yanoska ^b   Guisan, Jose M ^b  

^a INSTITUTO DE FERMENTACIONES INDUSTRIALES CSIC   (Spain)

^b INSTITUTO DE CATÁLISIS Y PETROLEOQUÍMICA   (Spain)

^c PONTIFICIA UNIVERSIDAD CATÓLICA DE VALPARAÍSO   (Chile)

^d UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

Author keywords

Enzyme immobilization; Hydrolysis of sardine oil; Omega 3 fatty acids; Polyfunctional polymers; Rhizomucor miehei lipase; Selective release of eicosapentenoic acid

Indexed keywords

DESORPTION; ENZYME IMMOBILIZATION; HYDROLYSIS; HYDROPHOBICITY; ISOMERS; LIPASES; SOAPS (DETERGENTS); UNSATURATED FATTY ACIDS;

EICOSAPENTAENOIC ACID; HYDROLYTIC ACTIVITIES; HYDROPHOBIC SUPPORTS; OMEGA-3-FATTY ACIDS; POLYFUNCTIONAL; RHIZOMUCOR MIEHEI LIPASE; SARDINE OIL; SELECTIVE HYDROLYSIS;

CROSSLINKING;

RHIZOMUCOR; RHIZOMUCOR MIEHEI;

EID: 79958789761     PISSN: 0003021X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11746-010-1727-2     Document Type: Article

References (21)

1. Reetz MT (2002) Lipases as practical biocatalysts. Curr Opin Chem Biol 6:145-150 (Pubitemid 34251368)
2. Fernández-Lorente G, Pizarro C, López-Vela D, Betancor L, Carrascosa AC, Pessela B, Guisan M (2010) Hydrolysis of fish oil by lipases immobilized inside porous supports. J Am Oil Chem Soc (Submitted)
3. Nelson LA, Foglia TA, Marmer WN (1996) Lipase-catalyzed production of biodiesel. J Am Oil Chem Soc 73:1191-1195 (Pubitemid 126527383)
4. Akai S, Kita Y (2007) Recent progress on the lipase-catalyzed asymmetric syntheses. J Synth Org Chem 65:772-782 (Pubitemid 47628620)
5. Brady L, Brzozowski AM, Derewenda ZS, Dodson E, Dodson G, Tolley S, Turkenburg JP, Menge U (1990) A serine protease triad forms the catalytic centre of a triacylglycerol lipase. Nature 343:767-770
6. Brzozowski AM, Derewenda U, Derewenda ZS, Dodson GG, Lawson DM, Turkenburg JP, Bjorkling F, Thim L (1991) A model for interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex. Nature 351:491-494 (Pubitemid 21896627)
7. Derewenda U, Brzozowski AM, Lawson DM, Derewenda ZS (1992) Catalysis at the interface: The anatomy of a conformational change in a triglyceride lipase. Biochemistry 31:1532-1541
8. Bastida A, Sabuquillo P, Armisen P, Fernández-Lafuente R, Huguet J, Guisán JM (1998) A single step purification, immobilization, and hyperactivation of lipases via interfacial adsorption on strongly hydrophobic supports. Biotechnol Bioeng 58:486-493 (Pubitemid 28178250)
9. Palomo JM, Peñas MM, Fernández-Lorente G, Mateo C, Pisabarro AG, Fernández-Lafuente R, Ramírez L, Guisán JM (2003) Solid-phase handling of hydrophobins: immobilized hydrophobins as a new tool to study lipases. Biomacromolecules 4(2):204-210 (Pubitemid 36527089)
10. Fernandez L, Banuelos O, Zafra A, Ronchel C, Perez-Victoria I, Morales JC, Velasco J, Adrio JL (2008) Alteration of substrate specificity of Galactomyces geotrichum BT107 lipase I on eicosapentaenoic acid-rich triglycerides. Biocatal Biotransform 26(4):296-305 (Pubitemid 351877875)
11. Heird WC (2001) The role of polyunsaturated fatty acids in term and preterm infants and breastfeeding mothers. Pediatr Clin North Am 48(1):173-188 (Pubitemid 32193134)
12. Saremi A, Arora R (2009) The utility of omega-3 fatty acids in cardiovascular disease. Am J Ther 16(5):421-436
13. Fernández-Lorente G, Palomo JM, Cabrera Z, Fernández- Lafuente R, Guisán JM (2007) Improved catalytic properties of immobilized lipases by the presence of very low concentratio ns of detergents in the reaction medium. Biotechnol Bioeng 97:242-250 (Pubitemid 46852921)
14. Palomo JM, Muñoz G, Fernández-Lorente G, Mateo C, Fuentes M, Guisan JM (2003) Modulation of Mucor miehei lipase properties via directed immobilization on different hetero-functional epoxy resins: hydrolytic resolution of (R, S)-2-butyroyl-2-phenylacetic acid. J Mol Catal B Enzym 21(4-6):201-210 (Pubitemid 36057137)
15. Palomo JM, Segura RL, Fernández-Lorente G, Pernas M, Rua ML, Guisán JM, Fernández-Lafuente R (2004) Purification, immobilization, and stabilization of a lipase from Bacillus thermocatenulatus by interfacial adsorption on hydrophobic supports. Biotechnol Progr 20(2):630-635 (Pubitemid 38459172)
16. Bastida A, Sabuquillo P, Armisen P, Fernández-Lafuente R, Huguet J, Guisán JM (1998) A single step purification, immobilization, and hyperactivation of lipases via interfacial adsorption on strongly hydrophobic supports. Biotechnol Bioeng 58(5):486-493 (Pubitemid 28178250)
17. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72(1-2):248-254
18. Guisán JM, Rodríguez V, Rosell CM, Soler G, Bastida A, Fernández-Lafuente R (1997) Stabilization of immobilized enzymes by chemical modification with polyfunctional macromolecules. In: Bickerstaff GF (ed) Methods in biotechnology 1 immobilization of enzymes and cells. Humana Press, NJ, pp 289-297
19. Fernandez-Lorente G, Godoy CA, Mendes AA, Lopez-Gallego F, Grazu V, de las Rivas B, Palomo JM, Hermoso J, Fernandez- Lafuente R, Guisan JM (2008) Solid-phase chemical amination of a lipase from Bacillus thermocatenulatus to improve its stabilization via covalent immobilization on highly activated glyoxylagarose. Biomacromolecules 9(9):2553-2561
20. Lienqueo ME, Mahn A, Salgado JC, Asenjo JA (2007) Current insights on protein behaviour in hydrophobic interaction chromatography. J Chromat B 849:53-68 (Pubitemid 46529326)
21. Rosell CM, Terreni M, Fernandez-Lafuente R, Guisan JM (1998) A criterion for the selection of monophasic solvents for enzymatic synthesis. Enzyme Microb Technol 23(1-2):64-69 (Pubitemid 28379062)